The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi

Tinospora crispa is a traditional medicinal plant in Malaysia with anti-cancer properties as shown in recent studies. The main objective of this study was to determine the antiproliferative effect of T. crispa methanol extract on triple negative breast cancer. MTT assay was performed to determine th...

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Main Author:	Al-Rashidi, Reyadh Radhi
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Study and teaching
Online Access:	http://ir.uitm.edu.my/id/eprint/15561/ http://ir.uitm.edu.my/id/eprint/15561/1/TM_REYADH%20RADHI%20AL%20-RASHIDI%20MD%2013_5.PDF

id	uitm-15561
recordtype	eprints
spelling	uitm-155612016-12-02T04:40:17Z http://ir.uitm.edu.my/id/eprint/15561/ The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi Al-Rashidi, Reyadh Radhi Study and teaching Tinospora crispa is a traditional medicinal plant in Malaysia with anti-cancer properties as shown in recent studies. The main objective of this study was to determine the antiproliferative effect of T. crispa methanol extract on triple negative breast cancer. MTT assay was performed to determine the cell viability of triple negative breast cancer cell lines (MDA-MB-231 and HCC1806) and normal breast cell line (MCF-10A). The type of cell death was determined using flow cytometry and cellular DNA fragmentation ELISA while Comet assay was used to determine the genotoxicity. qPCR was used to investigate the mRNA expression levels of the caspases 3, 8, 9 and NF-kB. The present results showed that T. crispa decreased the cell viability in triple negative breast cancer cells in a dose dependent manner with an IC50 of 66±3/µg/ml and 60±4µg/ml in MDA-MB-231 and HCC1806 cells respectively. While for MCF-10A, the IC50 was 248±4µg/ml. The type of cell death in MDA-MB-231, HCC1806 and MCF-10A cells was mainly due to apoptosis. The comet assay data for T. crispa did not detect any DNA damage on MDA-MB-231, HCC1806 and MCF-10A cell lines. Our results also showed that cisplatin significantly up-regulated NF-kB gene expression. Many studies reported that the up-regulation of NFkB increases the resistance of cancer cells to apoptosis. Unlike cisplatin, T. crispa did not show any significant change in the mRNA expression levels of NF-kB. Furthermore, when used in combination T. crispa and cisplatin, the combination significantly downregulated the gene expression of NF-kB and significantly up-regulated the gene expression of caspases 3, 8 and 9 in the cancer cells compared to single usage indicating more apoptotic activity. In Conclusion, T. crispa showed anti-proliferative effect on triple negative breast cancer cells with less toxicity to the normal breast cells. The cell death was mainly due to apoptosis and the combination of T. crispa and cisplatin significantly down-regulated the NF-kB gene expression which in turn increased apoptosis in the cancer cell lines. 2013 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/15561/1/TM_REYADH%20RADHI%20AL%20-RASHIDI%20MD%2013_5.PDF Al-Rashidi, Reyadh Radhi (2013) The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Study and teaching
spellingShingle	Study and teaching Al-Rashidi, Reyadh Radhi The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi
description	Tinospora crispa is a traditional medicinal plant in Malaysia with anti-cancer properties as shown in recent studies. The main objective of this study was to determine the antiproliferative effect of T. crispa methanol extract on triple negative breast cancer. MTT assay was performed to determine the cell viability of triple negative breast cancer cell lines (MDA-MB-231 and HCC1806) and normal breast cell line (MCF-10A). The type of cell death was determined using flow cytometry and cellular DNA fragmentation ELISA while Comet assay was used to determine the genotoxicity. qPCR was used to investigate the mRNA expression levels of the caspases 3, 8, 9 and NF-kB. The present results showed that T. crispa decreased the cell viability in triple negative breast cancer cells in a dose dependent manner with an IC50 of 66±3/µg/ml and 60±4µg/ml in MDA-MB-231 and HCC1806 cells respectively. While for MCF-10A, the IC50 was 248±4µg/ml. The type of cell death in MDA-MB-231, HCC1806 and MCF-10A cells was mainly due to apoptosis. The comet assay data for T. crispa did not detect any DNA damage on MDA-MB-231, HCC1806 and MCF-10A cell lines. Our results also showed that cisplatin significantly up-regulated NF-kB gene expression. Many studies reported that the up-regulation of NFkB increases the resistance of cancer cells to apoptosis. Unlike cisplatin, T. crispa did not show any significant change in the mRNA expression levels of NF-kB. Furthermore, when used in combination T. crispa and cisplatin, the combination significantly downregulated the gene expression of NF-kB and significantly up-regulated the gene expression of caspases 3, 8 and 9 in the cancer cells compared to single usage indicating more apoptotic activity. In Conclusion, T. crispa showed anti-proliferative effect on triple negative breast cancer cells with less toxicity to the normal breast cells. The cell death was mainly due to apoptosis and the combination of T. crispa and cisplatin significantly down-regulated the NF-kB gene expression which in turn increased apoptosis in the cancer cell lines.
format	Thesis
author	Al-Rashidi, Reyadh Radhi
author_facet	Al-Rashidi, Reyadh Radhi
author_sort	Al-Rashidi, Reyadh Radhi
title	The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi
title_short	The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi
title_full	The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi
title_fullStr	The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi
title_full_unstemmed	The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi
title_sort	anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / reyadh radhi al-rashidi
publishDate	2013
url	http://ir.uitm.edu.my/id/eprint/15561/ http://ir.uitm.edu.my/id/eprint/15561/1/TM_REYADH%20RADHI%20AL%20-RASHIDI%20MD%2013_5.PDF
first_indexed	2023-09-18T22:54:07Z
last_indexed	2023-09-18T22:54:07Z
_version_	1777417739782062080

The anti proliferative roperties of tinospora crispa on triple negative breast cancer cell lines / Reyadh Radhi Al-Rashidi

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