Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi

While gentamicin is effective against severe gram-negative infections, its nephrotoxicity restricts clinical applications. This study was aimed at investigating effect of Cassia auriculata aqueous and polyphenolic extracts against gentamicininduced nephrotoxicity. Polyphenolic extract was prepared...

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Main Author:	Farshbafi, Manizheh Khalilpour
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Biochemistry QR Microbiology
Online Access:	http://ir.uitm.edu.my/id/eprint/15724/ http://ir.uitm.edu.my/id/eprint/15724/1/TM_MANIZHEH%20KHALILPOUR%20FARSHBAFI%20PH%2015_5.PDF

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recordtype	eprints
spelling	uitm-157242017-01-09T02:04:02Z http://ir.uitm.edu.my/id/eprint/15724/ Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi Farshbafi, Manizheh Khalilpour Biochemistry QR Microbiology While gentamicin is effective against severe gram-negative infections, its nephrotoxicity restricts clinical applications. This study was aimed at investigating effect of Cassia auriculata aqueous and polyphenolic extracts against gentamicininduced nephrotoxicity. Polyphenolic extract was prepared by extracting flower aqueous extract with ethyl acetate (1:1 v/v). Effect of C. auriculata aqueous or polyphenolic extracts on cellular viability and H2C>2 -induced cell death on Vero cells were measured by (MTS) assay. To examine nephroprotective effect, male Sprague- Dawley rats were pretreated with different doses of C. auriculata aqueous (100, 300,500 mg/kg/day, p.o.) or polyphenolic extracts (50, 100, 300 mg/kg/day, p.o.) for 2 weeks, followed by 8 days treatment with gentamicin (100 mg/kg, i.p.). To examine therapeutic effect, rats were injected with gentamicin (100 mg/kg, i.p.) for 8 days,followed by treatment with different doses of C. auriculata aqueous (100, 300, 500 mg/kg/day, p.o.) or polyphenolic extracts (50, 100, 300 mg/kg/day, p.o.) for 2 weeks. On day 22, rats were placed individually in metabolic cages and 24 h urine samples were collected. On day 23, rats were killed and blood was collected. Renal function was assessed by serum urea, creatinine, albumin, total protein, urine creatinine,urinary protein excretion and creatinine clearance ratio. Kidney was subjected to histopathologic examination. Expression levels of antioxidant genes (catalase,superoxide dismutase, glutathione peroxidase) and proinflammatory cytokines (IL-1 (3,TNF-a) were measured by RT-PCR. In vitro results indicated that pretreatment of Vero cells with both aqueous and polyphenolic extracts for 24 h significantly ameliorated the cytotoxicity of H2O2 in a concentration-dependent manner. Aqueous extract produced a maximal protection afforded by 50 pg/mL at which, cell viability was 67%. Polyphenolic extract showed higher protective effect as compared to aqueous extract with the maximum protection at 50-200 pg/mL with about 73% cell viability. In vivo results indicated that C. auriculata aqueous (300 mg/kg) and polyphenolic (100 mg/kg) extracts significantly reduced gentamicine-induced elevations in serum creatinine, serum urea and urine total protein. Gentamicin-induced decrease in serum total protein, serum albumin, urine creatinine, and creatinine clearance were restored. Histopathological evaluation of renal cortex of rats treated with C. auriculata aqueous or polyphenolic extracts showed protective and therapeutic effect against tubular necrosis, infiltration of inflammatory cells and thickening of basement membrane. Antioxidant genes were significantly up-regulated by C.auriculata aqueous or polyphenolic extracts. Gentamicin-induced excessive expression of proinflammmatory cytokines were normalized by C. auriculata aqueous and polyphenolic extracts. The findings suggest that C. auriculata aqueous (300 mg/kg) and polyphenolic (100 mg/kg) extracts possess nephronprotective and nephrocurative activities by enhancing renal antioxidant enzymes and inhibiting proinflammmatory cytokines. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/15724/1/TM_MANIZHEH%20KHALILPOUR%20FARSHBAFI%20PH%2015_5.PDF Farshbafi, Manizheh Khalilpour (2015) Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Biochemistry QR Microbiology
spellingShingle	Biochemistry QR Microbiology Farshbafi, Manizheh Khalilpour Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi
description	While gentamicin is effective against severe gram-negative infections, its nephrotoxicity restricts clinical applications. This study was aimed at investigating effect of Cassia auriculata aqueous and polyphenolic extracts against gentamicininduced nephrotoxicity. Polyphenolic extract was prepared by extracting flower aqueous extract with ethyl acetate (1:1 v/v). Effect of C. auriculata aqueous or polyphenolic extracts on cellular viability and H2C>2 -induced cell death on Vero cells were measured by (MTS) assay. To examine nephroprotective effect, male Sprague- Dawley rats were pretreated with different doses of C. auriculata aqueous (100, 300,500 mg/kg/day, p.o.) or polyphenolic extracts (50, 100, 300 mg/kg/day, p.o.) for 2 weeks, followed by 8 days treatment with gentamicin (100 mg/kg, i.p.). To examine therapeutic effect, rats were injected with gentamicin (100 mg/kg, i.p.) for 8 days,followed by treatment with different doses of C. auriculata aqueous (100, 300, 500 mg/kg/day, p.o.) or polyphenolic extracts (50, 100, 300 mg/kg/day, p.o.) for 2 weeks. On day 22, rats were placed individually in metabolic cages and 24 h urine samples were collected. On day 23, rats were killed and blood was collected. Renal function was assessed by serum urea, creatinine, albumin, total protein, urine creatinine,urinary protein excretion and creatinine clearance ratio. Kidney was subjected to histopathologic examination. Expression levels of antioxidant genes (catalase,superoxide dismutase, glutathione peroxidase) and proinflammatory cytokines (IL-1 (3,TNF-a) were measured by RT-PCR. In vitro results indicated that pretreatment of Vero cells with both aqueous and polyphenolic extracts for 24 h significantly ameliorated the cytotoxicity of H2O2 in a concentration-dependent manner. Aqueous extract produced a maximal protection afforded by 50 pg/mL at which, cell viability was 67%. Polyphenolic extract showed higher protective effect as compared to aqueous extract with the maximum protection at 50-200 pg/mL with about 73% cell viability. In vivo results indicated that C. auriculata aqueous (300 mg/kg) and polyphenolic (100 mg/kg) extracts significantly reduced gentamicine-induced elevations in serum creatinine, serum urea and urine total protein. Gentamicin-induced decrease in serum total protein, serum albumin, urine creatinine, and creatinine clearance were restored. Histopathological evaluation of renal cortex of rats treated with C. auriculata aqueous or polyphenolic extracts showed protective and therapeutic effect against tubular necrosis, infiltration of inflammatory cells and thickening of basement membrane. Antioxidant genes were significantly up-regulated by C.auriculata aqueous or polyphenolic extracts. Gentamicin-induced excessive expression of proinflammmatory cytokines were normalized by C. auriculata aqueous and polyphenolic extracts. The findings suggest that C. auriculata aqueous (300 mg/kg) and polyphenolic (100 mg/kg) extracts possess nephronprotective and nephrocurative activities by enhancing renal antioxidant enzymes and inhibiting proinflammmatory cytokines.
format	Thesis
author	Farshbafi, Manizheh Khalilpour
author_facet	Farshbafi, Manizheh Khalilpour
author_sort	Farshbafi, Manizheh Khalilpour
title	Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi
title_short	Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi
title_full	Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi
title_fullStr	Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi
title_full_unstemmed	Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi
title_sort	effect of cassia auriculata (caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / manizheh khalilpour farshbafi
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/15724/ http://ir.uitm.edu.my/id/eprint/15724/1/TM_MANIZHEH%20KHALILPOUR%20FARSHBAFI%20PH%2015_5.PDF
first_indexed	2023-09-18T22:54:30Z
last_indexed	2023-09-18T22:54:30Z
_version_	1777417763457859584

Effect of Cassia auriculata (Caesalpiniaceae) flowers on gentamicin-induced nephrotoxicity in rats / Manizheh Khalilpour Farshbafi

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