Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni

Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutr...

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Main Author:	Rosni, Nordalilah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Online Access:	http://ir.uitm.edu.my/id/eprint/15907/ http://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF

id	uitm-15907
recordtype	eprints
spelling	uitm-159072016-12-19T07:39:27Z http://ir.uitm.edu.my/id/eprint/15907/ Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni Rosni, Nordalilah Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutritional benefits of PP such as neuroprotection, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) inhibition and antimicrobial properties. Neuroprotection properties were observed in infant brain cancer; human neuroblastoma cell line SH-SY5Y differentiated by treatment of retinoic acid and induced with H2O2. The samples gave negative neurotoxicity results and promising neuroprotective effects on 80% confluent SHSY5Y cell line in 96 well plates at a dose dependent manner. PT99 displayed most protection against H2 0 2 -induced cell lines and followed by T24 at 100 (ig/mL (p<0.05). The HMGR inhibition assay aims to find the cholesterol lowering ability of PP using pravastatin, a known HMGR inhibitor as control. T24/5 gave a considerably better oxidation of NADPH at 0.159 Units/mgP, followed by PT99/5 (0.009 Units/mgP) as compared to pravastatin. Antimicrobial testing against selected pathogenic bacteria and fungi infecting human were performed using well diffusion method at concentrations of 200 (ig/ml, 300 (ig/ml, 500 (xg/ml, 800 fig/ml and 1 mg/ml. The test showed negative results as no inhibitory activities were observed. The HPLC analysis showed that the four vitamin E derivatives identified in all PP samples were a-T3, y-T3, 8-T3 and a-TP. T24 exhibited significantly (p<0.05) higher total amount of vitamin E as compared to PT99. In conclusion, PP can be used as a healthy value-added product for human consumption. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF Rosni, Nordalilah (2015) Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
spellingShingle	Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Rosni, Nordalilah Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
description	Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutritional benefits of PP such as neuroprotection, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) inhibition and antimicrobial properties. Neuroprotection properties were observed in infant brain cancer; human neuroblastoma cell line SH-SY5Y differentiated by treatment of retinoic acid and induced with H2O2. The samples gave negative neurotoxicity results and promising neuroprotective effects on 80% confluent SHSY5Y cell line in 96 well plates at a dose dependent manner. PT99 displayed most protection against H2 0 2 -induced cell lines and followed by T24 at 100 (ig/mL (p<0.05). The HMGR inhibition assay aims to find the cholesterol lowering ability of PP using pravastatin, a known HMGR inhibitor as control. T24/5 gave a considerably better oxidation of NADPH at 0.159 Units/mgP, followed by PT99/5 (0.009 Units/mgP) as compared to pravastatin. Antimicrobial testing against selected pathogenic bacteria and fungi infecting human were performed using well diffusion method at concentrations of 200 (ig/ml, 300 (ig/ml, 500 (xg/ml, 800 fig/ml and 1 mg/ml. The test showed negative results as no inhibitory activities were observed. The HPLC analysis showed that the four vitamin E derivatives identified in all PP samples were a-T3, y-T3, 8-T3 and a-TP. T24 exhibited significantly (p<0.05) higher total amount of vitamin E as compared to PT99. In conclusion, PP can be used as a healthy value-added product for human consumption.
format	Thesis
author	Rosni, Nordalilah
author_facet	Rosni, Nordalilah
author_sort	Rosni, Nordalilah
title	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_short	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_full	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_fullStr	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_full_unstemmed	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_sort	neuroprotection, hmgr inhibition, vitamin e quantification and antimicrobial properties of palm puree / nordalilah rosni
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/15907/ http://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF
first_indexed	2023-09-18T22:54:55Z
last_indexed	2023-09-18T22:54:55Z
_version_	1777417789656530944

Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni

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