Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad

Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad

Watermelon is a natural product that contains high level of antioxidants and may prevent oxidative damage and apoptosis following an exposure to ionising radiation. The present study aimed to investigate the radioprotective role of 50 % watermelon juice against low dose radiation (LDR)-induced ox...

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Main Author: Mohammad, Mohd Khairul Amran
Format: Thesis
Language:English
Published: 2015
Subjects:
Medical physics. Medical radiology. Nuclear medicine
Online Access:http://ir.uitm.edu.my/id/eprint/15972/
http://ir.uitm.edu.my/id/eprint/15972/1/TM_MOHD%20KHAIRUL%20AMRAN%20MOHAMMAD%20HS%2015_5.pdf
id uitm-15972
recordtype eprints
spelling uitm-159722017-01-03T09:43:25Z http://ir.uitm.edu.my/id/eprint/15972/ Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad Mohammad, Mohd Khairul Amran Medical physics. Medical radiology. Nuclear medicine Watermelon is a natural product that contains high level of antioxidants and may prevent oxidative damage and apoptosis following an exposure to ionising radiation. The present study aimed to investigate the radioprotective role of 50 % watermelon juice against low dose radiation (LDR)-induced oxidative DNA damage and apoptosis in ICR mice. Eighteen male ICR mice were equally divided into three groups: control (Cx), radiation (Rx) and supplementation (Tx) groups (n=6). Cx and Rx received filtered tap water while Tx was supplemented with 50 % watermelon juice ad libitum for 28 consecutive days. A total body irradiation of 100 |iGy X-ray was given to Rx and Tx on day 29. Liver and lung tissues were assessed for the levels of reactive oxygen species (ROS) and DNA damage. The alteration of p53, Bax, and Bcl-2 protein expressions were determined followed by ultrastructural changes confirmation. Present study showed exposure to LDR was able to enhance oxidative DNA damage and alter the expression of p53, Bax and Bcl-2. The onset of apoptosis following LDR exposure was confirmed by ultrastructure evaluation of nucleus via transmission electron microscope (TEM). Interestingly, supplementation with 50 % watermelon juice prior to LDR exposure was able to diminish the levels of oxidative DNA damage compared to Cx. Additionally, mice in Tx showed significant restoration of altered apoptotic related protein expressions and reverted the abnormal structural changes to near normal morphology (Cx). These findings propose that 50 % watermelon juice may serve as an effective natural radioprotective agent against LDR-induced oxidative DNA damage and alteration of apoptotic-related protein expressions in ICR mice. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/15972/1/TM_MOHD%20KHAIRUL%20AMRAN%20MOHAMMAD%20HS%2015_5.pdf Mohammad, Mohd Khairul Amran (2015) Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad. Masters thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Medical physics. Medical radiology. Nuclear medicine
spellingShingle Medical physics. Medical radiology. Nuclear medicine
Mohammad, Mohd Khairul Amran
Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad
description Watermelon is a natural product that contains high level of antioxidants and may prevent oxidative damage and apoptosis following an exposure to ionising radiation. The present study aimed to investigate the radioprotective role of 50 % watermelon juice against low dose radiation (LDR)-induced oxidative DNA damage and apoptosis in ICR mice. Eighteen male ICR mice were equally divided into three groups: control (Cx), radiation (Rx) and supplementation (Tx) groups (n=6). Cx and Rx received filtered tap water while Tx was supplemented with 50 % watermelon juice ad libitum for 28 consecutive days. A total body irradiation of 100 |iGy X-ray was given to Rx and Tx on day 29. Liver and lung tissues were assessed for the levels of reactive oxygen species (ROS) and DNA damage. The alteration of p53, Bax, and Bcl-2 protein expressions were determined followed by ultrastructural changes confirmation. Present study showed exposure to LDR was able to enhance oxidative DNA damage and alter the expression of p53, Bax and Bcl-2. The onset of apoptosis following LDR exposure was confirmed by ultrastructure evaluation of nucleus via transmission electron microscope (TEM). Interestingly, supplementation with 50 % watermelon juice prior to LDR exposure was able to diminish the levels of oxidative DNA damage compared to Cx. Additionally, mice in Tx showed significant restoration of altered apoptotic related protein expressions and reverted the abnormal structural changes to near normal morphology (Cx). These findings propose that 50 % watermelon juice may serve as an effective natural radioprotective agent against LDR-induced oxidative DNA damage and alteration of apoptotic-related protein expressions in ICR mice.
format Thesis
author Mohammad, Mohd Khairul Amran
author_facet Mohammad, Mohd Khairul Amran
author_sort Mohammad, Mohd Khairul Amran
title Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad
title_short Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad
title_full Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad
title_fullStr Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad
title_full_unstemmed Radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative DNA damage and apoptosis in ICR mice / Mohd Khairul Amran Mohammad
title_sort radioprotective role of 50 % watermelon juice against low dose radiation-induced oxidative dna damage and apoptosis in icr mice / mohd khairul amran mohammad
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/15972/
http://ir.uitm.edu.my/id/eprint/15972/1/TM_MOHD%20KHAIRUL%20AMRAN%20MOHAMMAD%20HS%2015_5.pdf
first_indexed 2023-09-18T22:55:04Z
last_indexed 2023-09-18T22:55:04Z
_version_ 1777417799136706560

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