Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu

Listeria monocytogenes is a foodborne pathogenic bacteria causing severe systemic infection in susceptible individuals. This facultative intracellular pathogen infects host tissues using their flagella actin. Listeria monocytogenes propelled from one cell to another through the assembly of the flage...

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Main Authors:	Mustakim, Maimunah, Mat Issa, Zuraini, Prof Dr Son Radu
Format:	Research Reports
Language:	English
Published:	Research Management Institute (RMI) 2014
Online Access:	http://ir.uitm.edu.my/id/eprint/17273/ http://ir.uitm.edu.my/id/eprint/17273/2/LP_MAIMUNAH%20MUSTAKIM%20RMI%2014_5.pdf

id	uitm-17273
recordtype	eprints
spelling	uitm-172732019-08-22T04:15:48Z http://ir.uitm.edu.my/id/eprint/17273/ Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu Mustakim, Maimunah Mat Issa, Zuraini Prof Dr Son Radu Listeria monocytogenes is a foodborne pathogenic bacteria causing severe systemic infection in susceptible individuals. This facultative intracellular pathogen infects host tissues using their flagella actin. Listeria monocytogenes propelled from one cell to another through the assembly of the flagella actin with the host cells.The present study aims to examine the rearrangement of host cells cytoskeleton actin upon infection of Listeria monocytogenes. Human colon carcinoma (HCT 116) cell lines were infected with Listeria monocytogenes strains. Cells were stained withalexa fluor 635 phalloidin prior to observation by confocal laser scanning microscopy (CLSM) to examine cellular changes of the host cells upon infection with the bacteria. The infected cells showed actin rearrangement from filamentous network to aggregation features. The infected cells also showed reduced membrane ruffling. The present study demonstrated interaction of host cells actin with Listeria monocytogenes. Confocal laser scanning microscope (CLSM) examination revealed the cytoskeleton actin rearrangement in the infected host cells. Research Management Institute (RMI) 2014 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/17273/2/LP_MAIMUNAH%20MUSTAKIM%20RMI%2014_5.pdf Mustakim, Maimunah and Mat Issa, Zuraini and Prof Dr Son Radu (2014) Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu. [Research Reports] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
description	Listeria monocytogenes is a foodborne pathogenic bacteria causing severe systemic infection in susceptible individuals. This facultative intracellular pathogen infects host tissues using their flagella actin. Listeria monocytogenes propelled from one cell to another through the assembly of the flagella actin with the host cells.The present study aims to examine the rearrangement of host cells cytoskeleton actin upon infection of Listeria monocytogenes. Human colon carcinoma (HCT 116) cell lines were infected with Listeria monocytogenes strains. Cells were stained withalexa fluor 635 phalloidin prior to observation by confocal laser scanning microscopy (CLSM) to examine cellular changes of the host cells upon infection with the bacteria. The infected cells showed actin rearrangement from filamentous network to aggregation features. The infected cells also showed reduced membrane ruffling. The present study demonstrated interaction of host cells actin with Listeria monocytogenes. Confocal laser scanning microscope (CLSM) examination revealed the cytoskeleton actin rearrangement in the infected host cells.
format	Research Reports
author	Mustakim, Maimunah Mat Issa, Zuraini Prof Dr Son Radu
spellingShingle	Mustakim, Maimunah Mat Issa, Zuraini Prof Dr Son Radu Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu
author_facet	Mustakim, Maimunah Mat Issa, Zuraini Prof Dr Son Radu
author_sort	Mustakim, Maimunah
title	Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu
title_short	Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu
title_full	Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu
title_fullStr	Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu
title_full_unstemmed	Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu
title_sort	molecular analysis of host cells response against infection of virulent strains listeria monocytogenes / maimunah mustakim , zuraini mat issa and prof dr son radu
publisher	Research Management Institute (RMI)
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/17273/ http://ir.uitm.edu.my/id/eprint/17273/2/LP_MAIMUNAH%20MUSTAKIM%20RMI%2014_5.pdf
first_indexed	2023-09-18T22:57:56Z
last_indexed	2023-09-18T22:57:56Z
_version_	1777417979591393280

Molecular analysis of host cells response against infection of virulent strains Listeria monocytogenes / Maimunah Mustakim , Zuraini Mat Issa and Prof Dr Son Radu

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