Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi

Nanoemulsion is one of the alternative nano-lipid delivery systems of lipophilic active ingredients into the human skin in pharmaceutical and cosmetic applications. Palm vitamin E extract is a natural lipophilic active that contains a-, β-,δ-, γ-tocotrienols and a-tocopherol. Palm vitamin E has the...

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Main Author:	Bustami Effendi, Tommy Julianto
Format:	Thesis
Language:	English
Published:	2014
Online Access:	http://ir.uitm.edu.my/id/eprint/17376/ http://ir.uitm.edu.my/id/eprint/17376/2/ABS_TOMMY%20JULIANTO%20BUSTAMI%20EFFENDI%20TDRA%20VOL%206%20IGS_14.pdf

id	uitm-17376
recordtype	eprints
spelling	uitm-173762018-10-02T07:50:01Z http://ir.uitm.edu.my/id/eprint/17376/ Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi Bustami Effendi, Tommy Julianto Nanoemulsion is one of the alternative nano-lipid delivery systems of lipophilic active ingredients into the human skin in pharmaceutical and cosmetic applications. Palm vitamin E extract is a natural lipophilic active that contains a-, β-,δ-, γ-tocotrienols and a-tocopherol. Palm vitamin E has the potency as an antioxidant for delaying the skin ageing process in cosmetic application, although the skin absorption of palm vitamin E in conventional formulations is considered low. The high lipophilicity of palm vitamin E isomers causes the limitation in the skin permeation. The skin penetration can be enhanced by the loading of palm vitamin E extract in an oil phase of a nanolipid-delivery system such as nanoemulsion. Therefore, the potency of nanoemulsion loaded with palm vitamin E extract was studied. Palm vitamin E extract was loaded in two nanoemulsions prepared by two different techniques and then incorporated into polymeric hydrogels. The skin absorption and pharmacokinetics of vitamin E isomers loaded in nanoemulsion hydrogels were investigated. Liquid chromatography assay method of a-, δ-, γ-tocotrienols and a-tocopherol in various samples of the different stages of this study was successfully validated and revalidated. Nanoemulsion formulations were developed firstly by the formation of lyotropic phase system as a pro-nanoemulsion (nanophase gel) by heat mixing, and secondly by using self nano-emulsifying technique by cold mixing. Two oils were used in the nanoemulsion formulation development; palm oil was formulated as a nanoemulsion by using hot mixing technique and, Nigella saliva oil nanoemulsion was prepared by using cold mixing of self emulsifying system. Palm oil nanoemulsion was formulated with the aid of design experiment as semisolid hydrogel by loading carbomers (Carbopol 934 and 940) and then characterized by the droplet size distribution to determine the best formulation for loading with palm vitamin E extract. The effect of carbomers incorporated in nanoemulsion formulations on the droplet size distribution, semisolid rheology and texture was determined by using expert design experiment to select a stable formulation for permeation studies. The in vitro vitamin E isomers permeation through the polycarbonate membrane was investigated using Franz diffusion cells. The vitamin E isomers permeation through polycarbonate membrane depended on the pore size of membrane and the concentration of Carbopol 940 in nanoemulsion hydrogel formulations. The different formulation of nanoemulsion formulation has not shown any affect on the permeation profiles of vitamin E isomers. Finally, the in vivo skin penetration of a-, δ-, γ-tocotrienols and atocopherol loaded in nanoemulsion hydrogel by topical application on the hairless rat abdominal skin was investigated. The accumulative amount of vitamin E isomers in rat skin epidermis and the bioavailability of vitamin E isomers measured in the rat plasma showed that nanoemulsion hydrogels with small droplets size provided high skin absorption. 8-tocotrienol in the form of nanoemulsion hydrogel with mean droplet size of l00nm was preferably absorbed through the rat skin compared to γ-, octocotrienols and a-tocopherol. 2014 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/17376/2/ABS_TOMMY%20JULIANTO%20BUSTAMI%20EFFENDI%20TDRA%20VOL%206%20IGS_14.pdf Bustami Effendi, Tommy Julianto (2014) Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi. PhD thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
description	Nanoemulsion is one of the alternative nano-lipid delivery systems of lipophilic active ingredients into the human skin in pharmaceutical and cosmetic applications. Palm vitamin E extract is a natural lipophilic active that contains a-, β-,δ-, γ-tocotrienols and a-tocopherol. Palm vitamin E has the potency as an antioxidant for delaying the skin ageing process in cosmetic application, although the skin absorption of palm vitamin E in conventional formulations is considered low. The high lipophilicity of palm vitamin E isomers causes the limitation in the skin permeation. The skin penetration can be enhanced by the loading of palm vitamin E extract in an oil phase of a nanolipid-delivery system such as nanoemulsion. Therefore, the potency of nanoemulsion loaded with palm vitamin E extract was studied. Palm vitamin E extract was loaded in two nanoemulsions prepared by two different techniques and then incorporated into polymeric hydrogels. The skin absorption and pharmacokinetics of vitamin E isomers loaded in nanoemulsion hydrogels were investigated. Liquid chromatography assay method of a-, δ-, γ-tocotrienols and a-tocopherol in various samples of the different stages of this study was successfully validated and revalidated. Nanoemulsion formulations were developed firstly by the formation of lyotropic phase system as a pro-nanoemulsion (nanophase gel) by heat mixing, and secondly by using self nano-emulsifying technique by cold mixing. Two oils were used in the nanoemulsion formulation development; palm oil was formulated as a nanoemulsion by using hot mixing technique and, Nigella saliva oil nanoemulsion was prepared by using cold mixing of self emulsifying system. Palm oil nanoemulsion was formulated with the aid of design experiment as semisolid hydrogel by loading carbomers (Carbopol 934 and 940) and then characterized by the droplet size distribution to determine the best formulation for loading with palm vitamin E extract. The effect of carbomers incorporated in nanoemulsion formulations on the droplet size distribution, semisolid rheology and texture was determined by using expert design experiment to select a stable formulation for permeation studies. The in vitro vitamin E isomers permeation through the polycarbonate membrane was investigated using Franz diffusion cells. The vitamin E isomers permeation through polycarbonate membrane depended on the pore size of membrane and the concentration of Carbopol 940 in nanoemulsion hydrogel formulations. The different formulation of nanoemulsion formulation has not shown any affect on the permeation profiles of vitamin E isomers. Finally, the in vivo skin penetration of a-, δ-, γ-tocotrienols and atocopherol loaded in nanoemulsion hydrogel by topical application on the hairless rat abdominal skin was investigated. The accumulative amount of vitamin E isomers in rat skin epidermis and the bioavailability of vitamin E isomers measured in the rat plasma showed that nanoemulsion hydrogels with small droplets size provided high skin absorption. 8-tocotrienol in the form of nanoemulsion hydrogel with mean droplet size of l00nm was preferably absorbed through the rat skin compared to γ-, octocotrienols and a-tocopherol.
format	Thesis
author	Bustami Effendi, Tommy Julianto
spellingShingle	Bustami Effendi, Tommy Julianto Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi
author_facet	Bustami Effendi, Tommy Julianto
author_sort	Bustami Effendi, Tommy Julianto
title	Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi
title_short	Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi
title_full	Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi
title_fullStr	Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi
title_full_unstemmed	Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi
title_sort	nanoemulsion loaded with palm vitamin e for cosmeceutical applications / tommy julianto bustami effendi
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/17376/ http://ir.uitm.edu.my/id/eprint/17376/2/ABS_TOMMY%20JULIANTO%20BUSTAMI%20EFFENDI%20TDRA%20VOL%206%20IGS_14.pdf
first_indexed	2023-09-18T22:58:10Z
last_indexed	2023-09-18T22:58:10Z
_version_	1777417994307108864

Nanoemulsion loaded with palm vitamin E for cosmeceutical applications / Tommy Julianto Bustami Effendi

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