Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama

In this paper, immobilization of multi-enzyme using calcium alginate-clay beads for tapioca slurry saccharification process was carried out. The kinetics parameter and immobilization yield for alpha-amylase, glucoamylase, cellulose and multi-enzyme system were compared. The Michaelis constant, Km fo...

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Main Authors:	Abd Rahim, Siti Noraida, Sulaiman, Alawi, Edama, Nurul Aini
Format:	Article
Language:	English
Published:	Faculty of Mechanical Engineering (FKM) and UiTM Press 2014
Online Access:	http://ir.uitm.edu.my/id/eprint/17650/ http://ir.uitm.edu.my/id/eprint/17650/2/AJ_SITI%20NORAIDA%20ABD%20RAHIM%20JME%2014.pdf

id	uitm-17650
recordtype	eprints
spelling	uitm-176502019-08-22T03:51:46Z http://ir.uitm.edu.my/id/eprint/17650/ Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama Abd Rahim, Siti Noraida Sulaiman, Alawi Edama, Nurul Aini In this paper, immobilization of multi-enzyme using calcium alginate-clay beads for tapioca slurry saccharification process was carried out. The kinetics parameter and immobilization yield for alpha-amylase, glucoamylase, cellulose and multi-enzyme system were compared. The Michaelis constant, Km for free alpha-amylase, glucoamylase, cellulase and multi-enzyme were 2.0831,1.8326, 7.8592 and 3.5367 mg/mL, respectively. As for the immobilized system, the Km values were 3.1604, 2.1708, 9.2791 and 4.91176 mg/mL, respectively. The Km value for the immobilized multi-enzyme system was 1.39 times higher than free multi-enzyme system. This suggests that the immobilized multi-enzyme is a potential system for tapioca saccharification process due to affinity of enzyme to the substrate. For the immobilization yield, the yield for alpha-amylase, glucoamylase, cellulase and multi-enzyme were 51.19, 50.21, 40.68 and 53.71 %, respectively. The result showed the immobilized multi-enzyme had a higher yield compared to the single enzyme which could be explained due to the synergistic action of alpha-amylase, glucoamylase and cellulose enzymes. Therefore, it can be concluded that the use of immobilized multi-enzyme system had a very good potential for starch bioconversion into glucose. Faculty of Mechanical Engineering (FKM) and UiTM Press 2014 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/17650/2/AJ_SITI%20NORAIDA%20ABD%20RAHIM%20JME%2014.pdf Abd Rahim, Siti Noraida and Sulaiman, Alawi and Edama, Nurul Aini (2014) Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama. Journal of Mechanical Engineering, 11 (2). pp. 67-77. ISSN 1823-5514
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
description	In this paper, immobilization of multi-enzyme using calcium alginate-clay beads for tapioca slurry saccharification process was carried out. The kinetics parameter and immobilization yield for alpha-amylase, glucoamylase, cellulose and multi-enzyme system were compared. The Michaelis constant, Km for free alpha-amylase, glucoamylase, cellulase and multi-enzyme were 2.0831,1.8326, 7.8592 and 3.5367 mg/mL, respectively. As for the immobilized system, the Km values were 3.1604, 2.1708, 9.2791 and 4.91176 mg/mL, respectively. The Km value for the immobilized multi-enzyme system was 1.39 times higher than free multi-enzyme system. This suggests that the immobilized multi-enzyme is a potential system for tapioca saccharification process due to affinity of enzyme to the substrate. For the immobilization yield, the yield for alpha-amylase, glucoamylase, cellulase and multi-enzyme were 51.19, 50.21, 40.68 and 53.71 %, respectively. The result showed the immobilized multi-enzyme had a higher yield compared to the single enzyme which could be explained due to the synergistic action of alpha-amylase, glucoamylase and cellulose enzymes. Therefore, it can be concluded that the use of immobilized multi-enzyme system had a very good potential for starch bioconversion into glucose.
format	Article
author	Abd Rahim, Siti Noraida Sulaiman, Alawi Edama, Nurul Aini
spellingShingle	Abd Rahim, Siti Noraida Sulaiman, Alawi Edama, Nurul Aini Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama
author_facet	Abd Rahim, Siti Noraida Sulaiman, Alawi Edama, Nurul Aini
author_sort	Abd Rahim, Siti Noraida
title	Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama
title_short	Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama
title_full	Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama
title_fullStr	Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama
title_full_unstemmed	Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama
title_sort	kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with sg. sayong clay / siti noraida abd rahim, alawi sulaiman and nurul aini edama
publisher	Faculty of Mechanical Engineering (FKM) and UiTM Press
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/17650/ http://ir.uitm.edu.my/id/eprint/17650/2/AJ_SITI%20NORAIDA%20ABD%20RAHIM%20JME%2014.pdf
first_indexed	2023-09-18T22:58:45Z
last_indexed	2023-09-18T22:58:45Z
_version_	1777418031175041024

Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama

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