Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali

Erythroxylum cuneatum (Erythroxylaceae) standardized aqueous extract was investigated for its antioxidant and antihyperglycemic activities for its safety in vivo. Dried extract was provided by Forest Research Institute of Malaysia (FRIM). Antioxidant activity was evaluated via total phenolic content...

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Main Author:	Ali, Roslina
Format:	Thesis
Language:	English
Published:	2016
Online Access:	http://ir.uitm.edu.my/id/eprint/18530/ http://ir.uitm.edu.my/id/eprint/18530/2/TM_ROSLINA%20ALI%20PH%2016_5.pdf

id	uitm-18530
recordtype	eprints
spelling	uitm-185302018-10-16T02:58:43Z http://ir.uitm.edu.my/id/eprint/18530/ Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali Ali, Roslina Erythroxylum cuneatum (Erythroxylaceae) standardized aqueous extract was investigated for its antioxidant and antihyperglycemic activities for its safety in vivo. Dried extract was provided by Forest Research Institute of Malaysia (FRIM). Antioxidant activity was evaluated via total phenolic content assay, 1, 1 -diphenyl-2- picrylhydrazyl (DPPH) scavenging activity assay, ferric reducing antioxidant power (FRAP) assay, and via inhibitory effect of extract against lipid peroxidation in liver microsomes induced by tert-butylhydroperoxide (tert-BOOH). Safety of extract was determined using Organization for Economic Co-operation and Development (OECD) guidelines acutely and subacutely in mice. Total phenolic content of extract was 0.38 ±0.15 mg gallic acid equivalent (GAE)/mg dry weight of extract. DPPH assay showed E. cuneatum extract had an EC₅₀ of 6.17 ± 0.02 μg/mL, while the antioxidant references were trolox, gallic acid and quercetin which elicited EC50 values of 3.89 ± 0.09, 0.3 ± 0.07 and 2.69 ± 0.07 μg/mL, respectively. EC50 values for E. cuneatum extract, trolox and gallic acid in the FRAP assay were 3.72 ± 0.28, 12.09 ± 0.44 and 18.59 ± 0.21 (μmol Fe²⁺/ mg dry weight, respectively. IC50 value for E. cuneatum extract, trolox and gallic acid for inhibition of tert-BOOH-induced lipid peroxidation were 31.62 ± 0.10, 10.00 ± 0.21 and 12.59 ±0.12 mg/mL, respectively. Antioxidant activity of extract was correlated to total phenolic content. Based on Organization for Economic Co-operation and Development (OECD) guidelines, oral E. cuneatum extract is non-toxic as no adverse effect were seen at levels of more than 5 g/kg following acute oral administration and 1 g/kg following subacute administration in male and female mice. However, when administered acutely via intraperitoneal route, a dose of 300 mg/kg extract was moderately toxic, thus was harmful. In the streptozotocin induced hyperglycemic rat model, E. cuneatum extract (100 and 300 mg/kg, p.o.) administered twice daily elicited a significant decrease in serum glucose level after 3 weeks of treatment. In summary, E. cuneatum standardized aqueous extract given orally was safe in acute and subacute treatment. It has glucose-lowering effect in hyperglycaemic condition and showed high antioxidant activities in four different antioxidant assays. 2016 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/18530/2/TM_ROSLINA%20ALI%20PH%2016_5.pdf Ali, Roslina (2016) Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
description	Erythroxylum cuneatum (Erythroxylaceae) standardized aqueous extract was investigated for its antioxidant and antihyperglycemic activities for its safety in vivo. Dried extract was provided by Forest Research Institute of Malaysia (FRIM). Antioxidant activity was evaluated via total phenolic content assay, 1, 1 -diphenyl-2- picrylhydrazyl (DPPH) scavenging activity assay, ferric reducing antioxidant power (FRAP) assay, and via inhibitory effect of extract against lipid peroxidation in liver microsomes induced by tert-butylhydroperoxide (tert-BOOH). Safety of extract was determined using Organization for Economic Co-operation and Development (OECD) guidelines acutely and subacutely in mice. Total phenolic content of extract was 0.38 ±0.15 mg gallic acid equivalent (GAE)/mg dry weight of extract. DPPH assay showed E. cuneatum extract had an EC₅₀ of 6.17 ± 0.02 μg/mL, while the antioxidant references were trolox, gallic acid and quercetin which elicited EC50 values of 3.89 ± 0.09, 0.3 ± 0.07 and 2.69 ± 0.07 μg/mL, respectively. EC50 values for E. cuneatum extract, trolox and gallic acid in the FRAP assay were 3.72 ± 0.28, 12.09 ± 0.44 and 18.59 ± 0.21 (μmol Fe²⁺/ mg dry weight, respectively. IC50 value for E. cuneatum extract, trolox and gallic acid for inhibition of tert-BOOH-induced lipid peroxidation were 31.62 ± 0.10, 10.00 ± 0.21 and 12.59 ±0.12 mg/mL, respectively. Antioxidant activity of extract was correlated to total phenolic content. Based on Organization for Economic Co-operation and Development (OECD) guidelines, oral E. cuneatum extract is non-toxic as no adverse effect were seen at levels of more than 5 g/kg following acute oral administration and 1 g/kg following subacute administration in male and female mice. However, when administered acutely via intraperitoneal route, a dose of 300 mg/kg extract was moderately toxic, thus was harmful. In the streptozotocin induced hyperglycemic rat model, E. cuneatum extract (100 and 300 mg/kg, p.o.) administered twice daily elicited a significant decrease in serum glucose level after 3 weeks of treatment. In summary, E. cuneatum standardized aqueous extract given orally was safe in acute and subacute treatment. It has glucose-lowering effect in hyperglycaemic condition and showed high antioxidant activities in four different antioxidant assays.
format	Thesis
author	Ali, Roslina
spellingShingle	Ali, Roslina Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali
author_facet	Ali, Roslina
author_sort	Ali, Roslina
title	Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali
title_short	Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali
title_full	Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali
title_fullStr	Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali
title_full_unstemmed	Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali
title_sort	screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / roslina ali
publishDate	2016
url	http://ir.uitm.edu.my/id/eprint/18530/ http://ir.uitm.edu.my/id/eprint/18530/2/TM_ROSLINA%20ALI%20PH%2016_5.pdf
first_indexed	2023-09-18T23:00:44Z
last_indexed	2023-09-18T23:00:44Z
_version_	1777418155425005568

Screening for antioxidant activity, toxicity and anti-diabetic effect of erythroxylum cuneatum standardized aqueous extract / Roslina Ali

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