Cytological and molecular analysis of anti-tumour bioactivities of Urgines maritima (L.) baker aqueous extract on human malignant neuroblastoma with its neuroprotection ability / Maati Musbah Saleh Elghuol
This study was conducted with the hypothesis that the phytochemical closely related to Libyan medicinal herb, Urginea maritima (L.) Baker constituents within water-based extract would own appropriately powerful properties that contribute to intrinsic regulation specific antitumour activities in...
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| Format: | Book Section |
| Language: | English |
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Institute of Graduate Studies
2017
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| Online Access: | http://ir.uitm.edu.my/id/eprint/18922/ http://ir.uitm.edu.my/id/eprint/18922/1/ABS_MAATI%20MUSBAH%20SALEH%20ELGHUOL%20TDRA%20VOL%2012%20IGS%2017.pdf |
| Summary: | This study was conducted with the hypothesis that the phytochemical
closely related to Libyan medicinal herb, Urginea maritima (L.) Baker
constituents within water-based extract would own appropriately powerful
properties that contribute to intrinsic regulation specific antitumour
activities in human malignant SH-SY5Y neuroblastoma cells. The purpose
of the present study was to explore the characterisation and identification of
the major phytochemical of the U. maritima aqueous extract. Additionally,
the study also aimed to evaluate their effectiveness on the cellular and
molecular mechanism associated with the main anti-tumour criteria.
Through utilising several in-vitro techniques on both the experimental cell
line models involving (2× 104 to 1× 106 cells/ml) with the quality of ≥
90% viability of SH-SY5Y neuroblastoma and RA differentiated (neuronmodel)
cells. Evaluation of the impact of the active extract ingredients
was conducted based on morphological observation, biochemical, cellular
function and gene expression profile, and the analysis was carried
out for its estimation within different concentrations and durations.
Neuroblastoma is a well-known extra-cranial solid tumour and one of the
most deadly malignancies in childhood. |
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