Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail

Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail

Antimicrobial resistance has been a looming threat ever since its conception and it has become one of the greatest global problems of the current era. Although various studies have been conducted to better understand the mutational triggers leading to antimicrobial resistance, the specific genomic p...

Full description

Bibliographic Details
Main Author: Ismail, Mohamad Izwan
Format: Book Section
Language:English
Published: Institute of Graduate Studies, UiTM 2016
Subjects:
Malaysia
Online Access:http://ir.uitm.edu.my/id/eprint/19678/
http://ir.uitm.edu.my/id/eprint/19678/1/ABS_MOHAMAD%20IZWAN%20ISMAIL%20TDRA%20VOL%209%20IGS%2016.pdf
id uitm-19678
recordtype eprints
spelling uitm-196782018-06-07T05:42:19Z http://ir.uitm.edu.my/id/eprint/19678/ Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail Ismail, Mohamad Izwan Malaysia Antimicrobial resistance has been a looming threat ever since its conception and it has become one of the greatest global problems of the current era. Although various studies have been conducted to better understand the mutational triggers leading to antimicrobial resistance, the specific genomic path towards it have yet to be discerned. Here, we aim to elucidate the pathway of genomic evolution throughout the resistance induction of an A. baumannii strain towards ciprofloxacin, erythromycin, meropenem and imipenem, as well as comparing the mutations acquired clinically versus in vitro. Twenty-five (25) local clinical A. baumannii strains were isolated and screened for antimicrobial susceptibility, and their genome were sequenced using the Illumina GAIIx genome sequencer. The susceptible parent was then challenged with ciprofloxacin, erythromycin, meropenem and imipenem separately until growth is still possible beyond the Minimum Inhibitory Concentration (MIC) as defined by EUCAST standards. Once the resistance stability was confirmed, another sequencing run was done on the isogenic. Variant analysis was carried out using CLC Bio, and primers were designed to target the mutations of interest. PCR was then carried out on aliquots of the resistant mutants, each taken at increasing levels of antimicrobial tolerance throughout the challenging process. Phylogenomics and wgMLST analyses were carried out between the parent and resistant strain, as also the remaining isolates. Stable low and high-level resistant strains were successfully generated. Several genomic variants were identified in the high-level resistant strains… Institute of Graduate Studies, UiTM 2016 Book Section PeerReviewed text en http://ir.uitm.edu.my/id/eprint/19678/1/ABS_MOHAMAD%20IZWAN%20ISMAIL%20TDRA%20VOL%209%20IGS%2016.pdf Ismail, Mohamad Izwan (2016) Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail. In: The Doctoral Research Abstracts. IGS Biannual Publication, 9 (9). Institute of Graduate Studies, UiTM, Shah Alam.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Malaysia
spellingShingle Malaysia
Ismail, Mohamad Izwan
Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail
description Antimicrobial resistance has been a looming threat ever since its conception and it has become one of the greatest global problems of the current era. Although various studies have been conducted to better understand the mutational triggers leading to antimicrobial resistance, the specific genomic path towards it have yet to be discerned. Here, we aim to elucidate the pathway of genomic evolution throughout the resistance induction of an A. baumannii strain towards ciprofloxacin, erythromycin, meropenem and imipenem, as well as comparing the mutations acquired clinically versus in vitro. Twenty-five (25) local clinical A. baumannii strains were isolated and screened for antimicrobial susceptibility, and their genome were sequenced using the Illumina GAIIx genome sequencer. The susceptible parent was then challenged with ciprofloxacin, erythromycin, meropenem and imipenem separately until growth is still possible beyond the Minimum Inhibitory Concentration (MIC) as defined by EUCAST standards. Once the resistance stability was confirmed, another sequencing run was done on the isogenic. Variant analysis was carried out using CLC Bio, and primers were designed to target the mutations of interest. PCR was then carried out on aliquots of the resistant mutants, each taken at increasing levels of antimicrobial tolerance throughout the challenging process. Phylogenomics and wgMLST analyses were carried out between the parent and resistant strain, as also the remaining isolates. Stable low and high-level resistant strains were successfully generated. Several genomic variants were identified in the high-level resistant strains…
format Book Section
author Ismail, Mohamad Izwan
author_facet Ismail, Mohamad Izwan
author_sort Ismail, Mohamad Izwan
title Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail
title_short Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail
title_full Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail
title_fullStr Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail
title_full_unstemmed Genomic adaptation in antimicrobial resistance: Elucidating the route and effects in acinetobacter baumannii / Mohamad Izwan Ismail
title_sort genomic adaptation in antimicrobial resistance: elucidating the route and effects in acinetobacter baumannii / mohamad izwan ismail
publisher Institute of Graduate Studies, UiTM
publishDate 2016
url http://ir.uitm.edu.my/id/eprint/19678/
http://ir.uitm.edu.my/id/eprint/19678/1/ABS_MOHAMAD%20IZWAN%20ISMAIL%20TDRA%20VOL%209%20IGS%2016.pdf
first_indexed 2023-09-18T23:03:03Z
last_indexed 2023-09-18T23:03:03Z
_version_ 1777418300870885376

Similar Items