Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof

Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof

Timing of the first zygotic cleavage has been used as a marker of embryo developmental competence and subsequent viability. Previous studies showed that embryos that cleaved early had higher developmental viability. However, the factors contributing to timing of the first zygotic cleavage are unknow...

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Main Author: W. Jusof, Wan Hafizah
Format: Book Section
Language:English
Published: Institute of Graduate Studies, UiTM 2017
Subjects:
Malaysia
Online Access:http://ir.uitm.edu.my/id/eprint/19856/
http://ir.uitm.edu.my/id/eprint/19856/2/ABS_WAN%20HAFIZAH%20W.%20JUSOF%20TDRA%20VOL%2011%20IGS%2017.pdf
id uitm-19856
recordtype eprints
spelling uitm-198562018-06-08T02:02:02Z http://ir.uitm.edu.my/id/eprint/19856/ Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof W. Jusof, Wan Hafizah Malaysia Timing of the first zygotic cleavage has been used as a marker of embryo developmental competence and subsequent viability. Previous studies showed that embryos that cleaved early had higher developmental viability. However, the factors contributing to timing of the first zygotic cleavage are unknown. Energy production from mitochondria, nucleus and cytoskeletal organization might be some of the factors involved. Amino acid metabolic profiles might also relate with timing of the first zygotic cleavage as it has been reported to have significant relationships with embryo viability. Thus, the present study was designed to investigate the relationship between timing of the first zygotic cleavage, amino acid metabolic profiles, mitochondria, nucleus and cytoskeletal organization of mouse embryos with subsequent viability. Embryos were retrieved from superovulated ICR mice, 28 hours after hCG injection. At this point of time, 2-cell stage embryos were categorized as early-cleaving (EC), while zygotes with two pronuclei as late-cleaving (LC) embryos. Embryos were cultured overnight in M16 medium supplemented with 3% Bovine Serum Albumin (BSA) in a humidified carbon dioxide (CO2) incubator. For Experiment 1, both EC and LC embryos were divided into control and treatment groups. For control group, 2-cell stage embryos were cultured until the blastocyst stage. For treatment group, embryos were vitrified by EFS40 or EFS20/40 method for 1 hour and warmed. The vitrifiedwarmed embryos were cultured until the blastocyst stage. The number of surviving embryos and their development to the blastocyst were observed and counted… Institute of Graduate Studies, UiTM 2017 Book Section PeerReviewed text en http://ir.uitm.edu.my/id/eprint/19856/2/ABS_WAN%20HAFIZAH%20W.%20JUSOF%20TDRA%20VOL%2011%20IGS%2017.pdf W. Jusof, Wan Hafizah (2017) Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof. In: The Doctoral Research Abstracts. IGS Biannual Publication, 11 (11). Institute of Graduate Studies, UiTM, Shah Alam.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Malaysia
spellingShingle Malaysia
W. Jusof, Wan Hafizah
Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof
description Timing of the first zygotic cleavage has been used as a marker of embryo developmental competence and subsequent viability. Previous studies showed that embryos that cleaved early had higher developmental viability. However, the factors contributing to timing of the first zygotic cleavage are unknown. Energy production from mitochondria, nucleus and cytoskeletal organization might be some of the factors involved. Amino acid metabolic profiles might also relate with timing of the first zygotic cleavage as it has been reported to have significant relationships with embryo viability. Thus, the present study was designed to investigate the relationship between timing of the first zygotic cleavage, amino acid metabolic profiles, mitochondria, nucleus and cytoskeletal organization of mouse embryos with subsequent viability. Embryos were retrieved from superovulated ICR mice, 28 hours after hCG injection. At this point of time, 2-cell stage embryos were categorized as early-cleaving (EC), while zygotes with two pronuclei as late-cleaving (LC) embryos. Embryos were cultured overnight in M16 medium supplemented with 3% Bovine Serum Albumin (BSA) in a humidified carbon dioxide (CO2) incubator. For Experiment 1, both EC and LC embryos were divided into control and treatment groups. For control group, 2-cell stage embryos were cultured until the blastocyst stage. For treatment group, embryos were vitrified by EFS40 or EFS20/40 method for 1 hour and warmed. The vitrifiedwarmed embryos were cultured until the blastocyst stage. The number of surviving embryos and their development to the blastocyst were observed and counted…
format Book Section
author W. Jusof, Wan Hafizah
author_facet W. Jusof, Wan Hafizah
author_sort W. Jusof, Wan Hafizah
title Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof
title_short Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof
title_full Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof
title_fullStr Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof
title_full_unstemmed Relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / Wan Hafizah W. Jusof
title_sort relationship between timing of the first zygotic cleavage with cytoskeletal structures and amino acid metabolic profiles in vitrified mouse embryos / wan hafizah w. jusof
publisher Institute of Graduate Studies, UiTM
publishDate 2017
url http://ir.uitm.edu.my/id/eprint/19856/
http://ir.uitm.edu.my/id/eprint/19856/2/ABS_WAN%20HAFIZAH%20W.%20JUSOF%20TDRA%20VOL%2011%20IGS%2017.pdf
first_indexed 2023-09-18T23:03:26Z
last_indexed 2023-09-18T23:03:26Z
_version_ 1777418325000716288

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