Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari

Enterocytozoon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidiosis is also higher in immunocompetent individuals. The previous st...

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Main Author:	Azhari, Azah Ashikin
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Ethnic groups Public health. Hygiene. Preventive Medicine Health behavior and habits
Online Access:	http://ir.uitm.edu.my/id/eprint/20002/ http://ir.uitm.edu.my/id/eprint/20002/1/TD_AZAH%20ASHIKIN%20AZHARI%20HS%20B%2016_5.pdf

id	uitm-20002
recordtype	eprints
spelling	uitm-200022018-03-23T02:31:17Z http://ir.uitm.edu.my/id/eprint/20002/ Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari Azhari, Azah Ashikin Ethnic groups Public health. Hygiene. Preventive Medicine Health behavior and habits Enterocytozoon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidiosis is also higher in immunocompetent individuals. The previous study of microsporidial infection is mostly focused on immunodeficiency or immunosuppressive individuals. Information regarding microsporidial infections among immunocompetent individuals is lacking. Hence, this cross sectional study was carried out to determine prevalence of E. bieneusi infection and to find out the occurrence of E. bieneusi according to age group and gender among the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang, Malaysia. Stool samples were collected from 209 voluntary participants; 96 males and 113 females that aged between one to 85 years old. Demographic data, socioeconomic, environmental, and behavioral information was collected by using pre-tested questionnaire. Overall prevalence of E. bieneusi infection was 3.83 % (8/209). From the observation, majority of individual infected with E. bieneusi was male (8.33 %) can be observed in those aged ≥15 years old. These samples were detected Small Subunit rDNA gene by using Polymerase chain reaction amplification with specific primer EBIEF1 and EBIER1, as demonstrated by an amplicon of 607-bp. This is the first report of E. bieneusi in Malaysia. It is hoped that these findings will allow greater emphasis from public health authorities through public education on personal hygiene and the consumption of boiled or filtered water in order to reduce the infection. 2016 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/20002/1/TD_AZAH%20ASHIKIN%20AZHARI%20HS%20B%2016_5.pdf Azhari, Azah Ashikin (2016) Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari. Degree thesis, Universiti Teknologi MARA, Selangor, Puncak Alam Campus.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Ethnic groups Public health. Hygiene. Preventive Medicine Health behavior and habits
spellingShingle	Ethnic groups Public health. Hygiene. Preventive Medicine Health behavior and habits Azhari, Azah Ashikin Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
description	Enterocytozoon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidiosis is also higher in immunocompetent individuals. The previous study of microsporidial infection is mostly focused on immunodeficiency or immunosuppressive individuals. Information regarding microsporidial infections among immunocompetent individuals is lacking. Hence, this cross sectional study was carried out to determine prevalence of E. bieneusi infection and to find out the occurrence of E. bieneusi according to age group and gender among the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang, Malaysia. Stool samples were collected from 209 voluntary participants; 96 males and 113 females that aged between one to 85 years old. Demographic data, socioeconomic, environmental, and behavioral information was collected by using pre-tested questionnaire. Overall prevalence of E. bieneusi infection was 3.83 % (8/209). From the observation, majority of individual infected with E. bieneusi was male (8.33 %) can be observed in those aged ≥15 years old. These samples were detected Small Subunit rDNA gene by using Polymerase chain reaction amplification with specific primer EBIEF1 and EBIER1, as demonstrated by an amplicon of 607-bp. This is the first report of E. bieneusi in Malaysia. It is hoped that these findings will allow greater emphasis from public health authorities through public education on personal hygiene and the consumption of boiled or filtered water in order to reduce the infection.
format	Thesis
author	Azhari, Azah Ashikin
author_facet	Azhari, Azah Ashikin
author_sort	Azhari, Azah Ashikin
title	Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_short	Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_full	Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_fullStr	Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_full_unstemmed	Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_sort	identification and characterization of enterocytozoon bieneusi isolates from the proto-malay and senoi tribes in sungai lembing, pahang / azah ashikin azhari
publishDate	2016
url	http://ir.uitm.edu.my/id/eprint/20002/ http://ir.uitm.edu.my/id/eprint/20002/1/TD_AZAH%20ASHIKIN%20AZHARI%20HS%20B%2016_5.pdf
first_indexed	2023-09-18T23:03:45Z
last_indexed	2023-09-18T23:03:45Z
_version_	1777418345445851136

Identification and characterization of Enterocytozoon bieneusi isolates from the Proto-Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari

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