Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty

Osteosarcoma is the most common primary malignant bone tumour with a high resistance to chemo- and radiotherapy. Hyperthermia is a well-established type of cancer treatment. However, the molecular changes and responses of osteosarcoma cells to hyperthermia are not well understood. Hypothermia has be...

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Main Author:	Nashirty, Mohammed Ali Orba
Format:	Book Section
Language:	English
Published:	Institute of Graduate Studies, UiTM 2018
Subjects:	Examination. Diagnosis. Including radiography Diseases of the musculoskeletal system
Online Access:	http://ir.uitm.edu.my/id/eprint/20552/ http://ir.uitm.edu.my/id/eprint/20552/1/ABS_MOHAMMED%20ALI%20ORBA%20NASHIRTY%20TDRA%20VOL%2013%20IGS%2018.pdf

id	uitm-20552
recordtype	eprints
spelling	uitm-205522018-07-06T06:01:29Z http://ir.uitm.edu.my/id/eprint/20552/ Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty Nashirty, Mohammed Ali Orba Examination. Diagnosis. Including radiography Diseases of the musculoskeletal system Osteosarcoma is the most common primary malignant bone tumour with a high resistance to chemo- and radiotherapy. Hyperthermia is a well-established type of cancer treatment. However, the molecular changes and responses of osteosarcoma cells to hyperthermia are not well understood. Hypothermia has been proven to be protective in certain medical situations such as brain surgery, but there is no published study about its effect in bone cancer. According to literature, about 60% of all cancer patients take supplements without informing their oncologists during therapy. Little is known about the effect of antioxidants like berberine chloride on osteosarcoma cells, especially in combination with hyperthermia. Berberine is a natural alkaloid available in several traditional herbs, and it can help treat many pathological conditions. The overall objective of this study is to investigate the short- and long-term effects of various stages of hyper- and hypothermia on osteoblastlike osteosarcoma cells and its underlying mechanism of action. It also seeks to study the long-term effect of a single short-term treatment with severe hyperthermia (45°C, 1 h) on osteosarcoma cells and its underlying causes. An additional objective is to investigate the effect of hyperthermia alone and in combination with berberine chloride on osteosarcoma cells and its underlying mechanisms. Osteoblast-like osteosarcoma cells (MG-63 cells) were treated with hyper- and hypothermia for short, medium and long-term periods. Some cells were also treated with berberine chloride and a combination of berberine chloride with mild, moderate, and severe hyperthermia. Severe hypothermia and hyperthermia showed a time-dependent toxicity; hence viability was reduced in a significant manner at all time points, whereas mild hypothermia showed a protective effect... Institute of Graduate Studies, UiTM 2018 Book Section PeerReviewed text en http://ir.uitm.edu.my/id/eprint/20552/1/ABS_MOHAMMED%20ALI%20ORBA%20NASHIRTY%20TDRA%20VOL%2013%20IGS%2018.pdf Nashirty, Mohammed Ali Orba (2018) Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty. In: The Doctoral Research Abstracts. IGS Biannual Publication, 18 (18). Institute of Graduate Studies, UiTM, Shah Alam.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Examination. Diagnosis. Including radiography Diseases of the musculoskeletal system
spellingShingle	Examination. Diagnosis. Including radiography Diseases of the musculoskeletal system Nashirty, Mohammed Ali Orba Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty
description	Osteosarcoma is the most common primary malignant bone tumour with a high resistance to chemo- and radiotherapy. Hyperthermia is a well-established type of cancer treatment. However, the molecular changes and responses of osteosarcoma cells to hyperthermia are not well understood. Hypothermia has been proven to be protective in certain medical situations such as brain surgery, but there is no published study about its effect in bone cancer. According to literature, about 60% of all cancer patients take supplements without informing their oncologists during therapy. Little is known about the effect of antioxidants like berberine chloride on osteosarcoma cells, especially in combination with hyperthermia. Berberine is a natural alkaloid available in several traditional herbs, and it can help treat many pathological conditions. The overall objective of this study is to investigate the short- and long-term effects of various stages of hyper- and hypothermia on osteoblastlike osteosarcoma cells and its underlying mechanism of action. It also seeks to study the long-term effect of a single short-term treatment with severe hyperthermia (45°C, 1 h) on osteosarcoma cells and its underlying causes. An additional objective is to investigate the effect of hyperthermia alone and in combination with berberine chloride on osteosarcoma cells and its underlying mechanisms. Osteoblast-like osteosarcoma cells (MG-63 cells) were treated with hyper- and hypothermia for short, medium and long-term periods. Some cells were also treated with berberine chloride and a combination of berberine chloride with mild, moderate, and severe hyperthermia. Severe hypothermia and hyperthermia showed a time-dependent toxicity; hence viability was reduced in a significant manner at all time points, whereas mild hypothermia showed a protective effect...
format	Book Section
author	Nashirty, Mohammed Ali Orba
author_facet	Nashirty, Mohammed Ali Orba
author_sort	Nashirty, Mohammed Ali Orba
title	Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty
title_short	Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty
title_full	Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty
title_fullStr	Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty
title_full_unstemmed	Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty
title_sort	effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / mohammed ali orba nashirty
publisher	Institute of Graduate Studies, UiTM
publishDate	2018
url	http://ir.uitm.edu.my/id/eprint/20552/ http://ir.uitm.edu.my/id/eprint/20552/1/ABS_MOHAMMED%20ALI%20ORBA%20NASHIRTY%20TDRA%20VOL%2013%20IGS%2018.pdf
first_indexed	2023-09-18T23:04:49Z
last_indexed	2023-09-18T23:04:49Z
_version_	1777418412664815616

Effects of hypo- and hyperthermia, berberine and the combination of berberine with hyperthermia on osteosarcoma cells / Mohammed Ali Orba Nashirty

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