Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli

Cyanobacteria are photosynthetic organisms with relatively simple requirements for growth, when compared to other sources of biomass. The carbon source necessary for the cultivation of cyanobacteria represents up to 60% of the costs with nutrients. The cynanobacteria also are one of the richest sour...

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Main Authors:	Mohd Zan, Mohd Syahril, Othman, Roshani, Ramli, Nur Hasyimah
Format:	Research Reports
Language:	English
Published:	Research Management Institute (RMI) 2012
Subjects:	Cancer
Online Access:	http://ir.uitm.edu.my/id/eprint/21188/ http://ir.uitm.edu.my/id/eprint/21188/1/LP_MOHD%20SYAHRIL%20MOHD%20ZAN%20RMI%2012_5.pdf

id	uitm-21188
recordtype	eprints
spelling	uitm-211882018-08-14T02:19:47Z http://ir.uitm.edu.my/id/eprint/21188/ Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli Mohd Zan, Mohd Syahril Othman, Roshani Ramli, Nur Hasyimah Cancer Cyanobacteria are photosynthetic organisms with relatively simple requirements for growth, when compared to other sources of biomass. The carbon source necessary for the cultivation of cyanobacteria represents up to 60% of the costs with nutrients. The cynanobacteria also are one of the richest sources of biomedically relevant compounds with extensive therapeutic pharmaceutical applications (Gademann & Portman, 2008). Cyanobacteria metabolites show an interesting and exciting range of biological activities ranging from antimicrobial, immunosuppressant, anticancer and antiviral to proteinase-inhibiting activities which are striking targets of biomedical research (Luesch et al., 2002). Chemotherapy is one of the main treatments used to cure cancer. A great number of antitumor compounds are natural products or their derivatives, mainly produced by blue-green algae (Patterson et al, 1991, 1993). This study will be focus with an alternative treatment to produce new anticancer-type natural products from cyanobacteria. The proposed project will be focused on the isolation and purification of microalgae from natural habitat. Various culture media will be developed and modified such as AF6 medium (Watanabe et al., 2000), AK medium (Barsanti & Gualtieri, 2006), COMBO medium (Kilham et al., 1998) and Medium G (Blackburn et al., 2001) for isolation and cultivation of microalgae. The molecular-based techniques will be developed in monitoring, identification and quantification of microalgae species in mixed phytoplankton samples. The targets cyanobacteria will be growing rapidly are further subcultured and grow to a density sufficient for inoculation of scale-up volume. After cell densities of the scaled-up cultures are harvested, the cell material is freeze dried. The biomass is extracted using pure chloroform to obtain a crude lipid extract. This extracts will be evaluated for anticancer-type activity by using MTT assay. The standard chemotherapeutic agent doxorubicin will be used as a positive control in MTT assay and normal cell line L02 will be used to show whether cytotoxic activity was tumor-specific. The concentrations required to inhibit growth by 50% (IC50) will be calculated from survival curves using the Bliss method. This studies are to known the potential of cyanobacteria as anticancer and to screen cyanobacteria products for anticancer activity by MTT assay. It more to organic and new anticancer-type natural products which have no harmless to normal human cells. Research Management Institute (RMI) 2012 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/21188/1/LP_MOHD%20SYAHRIL%20MOHD%20ZAN%20RMI%2012_5.pdf Mohd Zan, Mohd Syahril and Othman, Roshani and Ramli, Nur Hasyimah (2012) Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli. [Research Reports] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Cancer
spellingShingle	Cancer Mohd Zan, Mohd Syahril Othman, Roshani Ramli, Nur Hasyimah Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli
description	Cyanobacteria are photosynthetic organisms with relatively simple requirements for growth, when compared to other sources of biomass. The carbon source necessary for the cultivation of cyanobacteria represents up to 60% of the costs with nutrients. The cynanobacteria also are one of the richest sources of biomedically relevant compounds with extensive therapeutic pharmaceutical applications (Gademann & Portman, 2008). Cyanobacteria metabolites show an interesting and exciting range of biological activities ranging from antimicrobial, immunosuppressant, anticancer and antiviral to proteinase-inhibiting activities which are striking targets of biomedical research (Luesch et al., 2002). Chemotherapy is one of the main treatments used to cure cancer. A great number of antitumor compounds are natural products or their derivatives, mainly produced by blue-green algae (Patterson et al, 1991, 1993). This study will be focus with an alternative treatment to produce new anticancer-type natural products from cyanobacteria. The proposed project will be focused on the isolation and purification of microalgae from natural habitat. Various culture media will be developed and modified such as AF6 medium (Watanabe et al., 2000), AK medium (Barsanti & Gualtieri, 2006), COMBO medium (Kilham et al., 1998) and Medium G (Blackburn et al., 2001) for isolation and cultivation of microalgae. The molecular-based techniques will be developed in monitoring, identification and quantification of microalgae species in mixed phytoplankton samples. The targets cyanobacteria will be growing rapidly are further subcultured and grow to a density sufficient for inoculation of scale-up volume. After cell densities of the scaled-up cultures are harvested, the cell material is freeze dried. The biomass is extracted using pure chloroform to obtain a crude lipid extract. This extracts will be evaluated for anticancer-type activity by using MTT assay. The standard chemotherapeutic agent doxorubicin will be used as a positive control in MTT assay and normal cell line L02 will be used to show whether cytotoxic activity was tumor-specific. The concentrations required to inhibit growth by 50% (IC50) will be calculated from survival curves using the Bliss method. This studies are to known the potential of cyanobacteria as anticancer and to screen cyanobacteria products for anticancer activity by MTT assay. It more to organic and new anticancer-type natural products which have no harmless to normal human cells.
format	Research Reports
author	Mohd Zan, Mohd Syahril Othman, Roshani Ramli, Nur Hasyimah
author_facet	Mohd Zan, Mohd Syahril Othman, Roshani Ramli, Nur Hasyimah
author_sort	Mohd Zan, Mohd Syahril
title	Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli
title_short	Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli
title_full	Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli
title_fullStr	Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli
title_full_unstemmed	Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli
title_sort	cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of chlorella vulgaris and spirulina platensis / mohd syahril mohd zan, roshani othman and nur hasyimah ramli
publisher	Research Management Institute (RMI)
publishDate	2012
url	http://ir.uitm.edu.my/id/eprint/21188/ http://ir.uitm.edu.my/id/eprint/21188/1/LP_MOHD%20SYAHRIL%20MOHD%20ZAN%20RMI%2012_5.pdf
first_indexed	2023-09-18T23:06:02Z
last_indexed	2023-09-18T23:06:02Z
_version_	1777418489260146688

Cultivation, evaluation of the anticancer activities and assessment of the phylogenetic selected strain of Chlorella vulgaris and Spirulina platensis / Mohd Syahril Mohd Zan, Roshani Othman and Nur Hasyimah Ramli

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