Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu

Listeria monocytogenes is one of the major food-borne pathogen with an opportunistic character that is able to cause severe human listeriosis worldwide. The development of a surveillance and detection system concerning food safety in this country needs to be improved. Therefore, this study was condu...

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Main Authors:	Mat Issa, Zuraini, Mustakim, Maimunah, Radu, Son
Format:	Research Reports
Language:	English
Published:	Research Management Institute (RMI) 2012
Subjects:	Microbial ecology
Online Access:	http://ir.uitm.edu.my/id/eprint/21555/ http://ir.uitm.edu.my/id/eprint/21555/1/LP_ZURAINA%20MAT%20ISSA%20RMI%2012_5.pdf

id	uitm-21555
recordtype	eprints
spelling	uitm-215552018-10-01T08:29:11Z http://ir.uitm.edu.my/id/eprint/21555/ Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu Mat Issa, Zuraini Mustakim, Maimunah Radu, Son Microbial ecology Listeria monocytogenes is one of the major food-borne pathogen with an opportunistic character that is able to cause severe human listeriosis worldwide. The development of a surveillance and detection system concerning food safety in this country needs to be improved. Therefore, this study was conducted to provide a general baseline on the contamination of L. monocytogenes in various foods in the local markets in Selangor. A total of 140 raw and ready-to-eat food samples were analyzed by plating on selective PALCAM medium and the suspected L. monocytogenes colonies were confirmed at molecular level by MPN-PCR technique using primers specific for this food-borne pathogen. All of the 23 isolated strains yield a PCR product of 938 bp and 701 bp for 16s rRNA gene and hylA virulence gene respectively. The confirmed isolates were characterized for their intra-species discrimination according to reaction between antigen-antisera by serotyping technique and patterns generated by RAPD bands. Two screened RAPD primers demonstrating potentially useful banding patterns were selected and examined against L monocytogenes isolates. Positive isolates were evaluated for their susceptibility to eight commonly used antibiotic agents by using disc diffusion assay. L monocytogenes was detected in 8.57% of collected food samples which 33.3%, 25% and 13.3% were detected in burger, minced meat and sausage samples respectively. The highest resistances (100%) of L monocytogenes isolates were demonstrated against ampicillin and penicillin G antibiotics, whereas high susceptibility was showed toward streptomycin (100%). The molecular approaches used in this study provide highly sensitive and specific results which were very easy and fast to perform. These findings also suggested that the contamination of L monocytogenes in foods is relatively low. However, it highlighted the emergence of antibiotics multi-resistant Listeria in the present environment. Research Management Institute (RMI) 2012 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/21555/1/LP_ZURAINA%20MAT%20ISSA%20RMI%2012_5.pdf Mat Issa, Zuraini and Mustakim, Maimunah and Radu, Son (2012) Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu. [Research Reports] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Microbial ecology
spellingShingle	Microbial ecology Mat Issa, Zuraini Mustakim, Maimunah Radu, Son Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu
description	Listeria monocytogenes is one of the major food-borne pathogen with an opportunistic character that is able to cause severe human listeriosis worldwide. The development of a surveillance and detection system concerning food safety in this country needs to be improved. Therefore, this study was conducted to provide a general baseline on the contamination of L. monocytogenes in various foods in the local markets in Selangor. A total of 140 raw and ready-to-eat food samples were analyzed by plating on selective PALCAM medium and the suspected L. monocytogenes colonies were confirmed at molecular level by MPN-PCR technique using primers specific for this food-borne pathogen. All of the 23 isolated strains yield a PCR product of 938 bp and 701 bp for 16s rRNA gene and hylA virulence gene respectively. The confirmed isolates were characterized for their intra-species discrimination according to reaction between antigen-antisera by serotyping technique and patterns generated by RAPD bands. Two screened RAPD primers demonstrating potentially useful banding patterns were selected and examined against L monocytogenes isolates. Positive isolates were evaluated for their susceptibility to eight commonly used antibiotic agents by using disc diffusion assay. L monocytogenes was detected in 8.57% of collected food samples which 33.3%, 25% and 13.3% were detected in burger, minced meat and sausage samples respectively. The highest resistances (100%) of L monocytogenes isolates were demonstrated against ampicillin and penicillin G antibiotics, whereas high susceptibility was showed toward streptomycin (100%). The molecular approaches used in this study provide highly sensitive and specific results which were very easy and fast to perform. These findings also suggested that the contamination of L monocytogenes in foods is relatively low. However, it highlighted the emergence of antibiotics multi-resistant Listeria in the present environment.
format	Research Reports
author	Mat Issa, Zuraini Mustakim, Maimunah Radu, Son
author_facet	Mat Issa, Zuraini Mustakim, Maimunah Radu, Son
author_sort	Mat Issa, Zuraini
title	Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu
title_short	Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu
title_full	Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu
title_fullStr	Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu
title_full_unstemmed	Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu
title_sort	characterization of listeria monocytogenes isolated from foods / zuraini mat issa, maimunah mustakim and son radu
publisher	Research Management Institute (RMI)
publishDate	2012
url	http://ir.uitm.edu.my/id/eprint/21555/ http://ir.uitm.edu.my/id/eprint/21555/1/LP_ZURAINA%20MAT%20ISSA%20RMI%2012_5.pdf
first_indexed	2023-09-18T23:06:49Z
last_indexed	2023-09-18T23:06:49Z
_version_	1777418538141614080

Characterization of Listeria monocytogenes isolated from foods / Zuraini Mat Issa, Maimunah Mustakim and Son Radu

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