Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad

Cryopreservation is a technique in order to preserve cell for long time storage. It is widely used in agricultural field to store male gametes in the use of liquid nitrogen. In this study, three adult bulls breed Piedmontase were used. Semen collected from these bulls was then grouped into two; trea...

Full description

Bibliographic Details
Main Authors:	Hj Mohd Yusof, Farida Zuraina, Syed Mohamad, Syarifah Faezah
Format:	Research Reports
Language:	English
Published:	Research Management Institute (RMI) 2012
Subjects:	Agricultural ecology (General) SB Plant culture
Online Access:	http://ir.uitm.edu.my/id/eprint/21559/ http://ir.uitm.edu.my/id/eprint/21559/1/LP_FARIDA%20ZURAINA%20MOHD%20YUSOF%20RMI%2012_5.pdf

id	uitm-21559
recordtype	eprints
spelling	uitm-215592018-10-01T08:09:01Z http://ir.uitm.edu.my/id/eprint/21559/ Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad Hj Mohd Yusof, Farida Zuraina Syed Mohamad, Syarifah Faezah Agricultural ecology (General) SB Plant culture Cryopreservation is a technique in order to preserve cell for long time storage. It is widely used in agricultural field to store male gametes in the use of liquid nitrogen. In this study, three adult bulls breed Piedmontase were used. Semen collected from these bulls was then grouped into two; treatment group was subjected to magnetic activated cell sorting system (MACS) and being cryopreserved, whilst the control group was directly cryopreserved without MACS. Sperm were incubated in Annexin V before placed in the column attached to the magnetic stand. Semen analysis was done by using the Computer Assisted Sperm Analysis software (CASA). Parameters included are sperm velocity (VAP, VCL, VSL; um/s) and sperm progression (WOB, LIN, STR; %). For sperm viability it was determined by eosin-nigrosin staining technique. Effects of different thawing temperature and time were analysed by using iQ™SYBR® Green assay for qPCR with GAPDH as reference gene. Cryocapacitation like damage was then identified by the chlorotetracycline/Hoechst staining assay under blue-violet illumination of fluorescent microscopy (excitation: 400-440nm, emission: 470nm). All statistical data were analysed using SPSS version 14; p-value was set at 0.05 as significant level. Findings revealed that thawing at 37°C for 13 seconds for MACS processed sample was superior compared to other thawing procedures. At 37°C, it produced significant data of VCL (xm/s, expression of HSP70 gene and CTC/Hoechst assay with p<0.05. As a conclusion, usage of MACS preceding cryopreservation can enhance cell survival by thawing at 37°C for 30 seconds. Research Management Institute (RMI) 2012 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/21559/1/LP_FARIDA%20ZURAINA%20MOHD%20YUSOF%20RMI%2012_5.pdf Hj Mohd Yusof, Farida Zuraina and Syed Mohamad, Syarifah Faezah (2012) Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad. [Research Reports] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Agricultural ecology (General) SB Plant culture
spellingShingle	Agricultural ecology (General) SB Plant culture Hj Mohd Yusof, Farida Zuraina Syed Mohamad, Syarifah Faezah Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad
description	Cryopreservation is a technique in order to preserve cell for long time storage. It is widely used in agricultural field to store male gametes in the use of liquid nitrogen. In this study, three adult bulls breed Piedmontase were used. Semen collected from these bulls was then grouped into two; treatment group was subjected to magnetic activated cell sorting system (MACS) and being cryopreserved, whilst the control group was directly cryopreserved without MACS. Sperm were incubated in Annexin V before placed in the column attached to the magnetic stand. Semen analysis was done by using the Computer Assisted Sperm Analysis software (CASA). Parameters included are sperm velocity (VAP, VCL, VSL; um/s) and sperm progression (WOB, LIN, STR; %). For sperm viability it was determined by eosin-nigrosin staining technique. Effects of different thawing temperature and time were analysed by using iQ™SYBR® Green assay for qPCR with GAPDH as reference gene. Cryocapacitation like damage was then identified by the chlorotetracycline/Hoechst staining assay under blue-violet illumination of fluorescent microscopy (excitation: 400-440nm, emission: 470nm). All statistical data were analysed using SPSS version 14; p-value was set at 0.05 as significant level. Findings revealed that thawing at 37°C for 13 seconds for MACS processed sample was superior compared to other thawing procedures. At 37°C, it produced significant data of VCL (xm/s, expression of HSP70 gene and CTC/Hoechst assay with p<0.05. As a conclusion, usage of MACS preceding cryopreservation can enhance cell survival by thawing at 37°C for 30 seconds.
format	Research Reports
author	Hj Mohd Yusof, Farida Zuraina Syed Mohamad, Syarifah Faezah
author_facet	Hj Mohd Yusof, Farida Zuraina Syed Mohamad, Syarifah Faezah
author_sort	Hj Mohd Yusof, Farida Zuraina
title	Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad
title_short	Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad
title_full	Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad
title_fullStr	Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad
title_full_unstemmed	Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad
title_sort	effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / farida zuraina hj mohd yusof and syarifah faezah syed mohamad
publisher	Research Management Institute (RMI)
publishDate	2012
url	http://ir.uitm.edu.my/id/eprint/21559/ http://ir.uitm.edu.my/id/eprint/21559/1/LP_FARIDA%20ZURAINA%20MOHD%20YUSOF%20RMI%2012_5.pdf
first_indexed	2023-09-18T23:06:49Z
last_indexed	2023-09-18T23:06:49Z
_version_	1777418538717282304

Effect of different thawing temperature and time on the viability of cryopreserved bovine spermatozoa / Farida Zuraina Hj Mohd Yusof and Syarifah Faezah Syed Mohamad

Similar Items