Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid

Low dose ionizing radiation (LDIR) may trigger oxidative stress which leads to generation of free radicals. There is lack of information about the capability of watermelon as a natural antioxidant that helps to scavenge LDIR-induced free radicals. This study was conducted to determine the radioprot...

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Main Author:	Abd Rasid, Anisah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Diseases of the lungs Radiography Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Online Access:	http://ir.uitm.edu.my/id/eprint/22292/ http://ir.uitm.edu.my/id/eprint/22292/1/TD_ANISAH%20ABD%20RASID%20HS%20B%2015_5.pdf

id	uitm-22292
recordtype	eprints
spelling	uitm-222922018-12-26T06:34:40Z http://ir.uitm.edu.my/id/eprint/22292/ Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid Abd Rasid, Anisah Diseases of the lungs Radiography Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Low dose ionizing radiation (LDIR) may trigger oxidative stress which leads to generation of free radicals. There is lack of information about the capability of watermelon as a natural antioxidant that helps to scavenge LDIR-induced free radicals. This study was conducted to determine the radioprotective properties of 50% watermelon juice on biochemical and molecular changes against LDIR-induced oxidative stress in mice lung. A total of 18 ICR male mice were randomly divided into three groups (n=6); negative control group, radiation group and treatment group. Mice in negative control and radiation group were given filtered tap water while treatment group was supplemented with 50% watermelon juice for 14 days ad libitum. Mice in radiation and treatment group were then exposed to 100 µGy x-ray whole body irradiation on day 15. Liver tissues were excised immediately and assessed for the superoxide dismutase (SOD) activity (inhibition %), total glutathione (GSH), malondialdehyde (MDA) and DNA damage (Comet Assay). SOD activity (inhibition %) showed no significant difference between all groups. Treatment group showed increment SOD activity (inhibition %) compared to negative control and radiation group. GSH level in lung tissues showed significant diminish in radiation group versus negative control group (p < 0.001). Treatment group showed significant depletion in GSH level compared to negative control group (p < 0.001). MDA levels showed significant increment in treatment group compared to negative control group (p < 0.005). DNA damage of lung tissues in radiation group showed significant increased compared to negative control (p < 0.001). While treatment group showed significant decreased in DNA damage compared to radiation group (p < 0.001). In conclusion, this finding may postulate that radioprotective properties of 50% watermelon juice against LDIR-induced oxidative stress which supplemented to the mice for 14 days may reduce DNA damage but may be not give sufficient enough effect to biochemical changes in lung tissue. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/22292/1/TD_ANISAH%20ABD%20RASID%20HS%20B%2015_5.pdf Abd Rasid, Anisah (2015) Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid. Degree thesis, Universiti Teknologi MARA, Selangor, Puncak Alam Campus.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Diseases of the lungs Radiography Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
spellingShingle	Diseases of the lungs Radiography Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Abd Rasid, Anisah Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid
description	Low dose ionizing radiation (LDIR) may trigger oxidative stress which leads to generation of free radicals. There is lack of information about the capability of watermelon as a natural antioxidant that helps to scavenge LDIR-induced free radicals. This study was conducted to determine the radioprotective properties of 50% watermelon juice on biochemical and molecular changes against LDIR-induced oxidative stress in mice lung. A total of 18 ICR male mice were randomly divided into three groups (n=6); negative control group, radiation group and treatment group. Mice in negative control and radiation group were given filtered tap water while treatment group was supplemented with 50% watermelon juice for 14 days ad libitum. Mice in radiation and treatment group were then exposed to 100 µGy x-ray whole body irradiation on day 15. Liver tissues were excised immediately and assessed for the superoxide dismutase (SOD) activity (inhibition %), total glutathione (GSH), malondialdehyde (MDA) and DNA damage (Comet Assay). SOD activity (inhibition %) showed no significant difference between all groups. Treatment group showed increment SOD activity (inhibition %) compared to negative control and radiation group. GSH level in lung tissues showed significant diminish in radiation group versus negative control group (p < 0.001). Treatment group showed significant depletion in GSH level compared to negative control group (p < 0.001). MDA levels showed significant increment in treatment group compared to negative control group (p < 0.005). DNA damage of lung tissues in radiation group showed significant increased compared to negative control (p < 0.001). While treatment group showed significant decreased in DNA damage compared to radiation group (p < 0.001). In conclusion, this finding may postulate that radioprotective properties of 50% watermelon juice against LDIR-induced oxidative stress which supplemented to the mice for 14 days may reduce DNA damage but may be not give sufficient enough effect to biochemical changes in lung tissue.
format	Thesis
author	Abd Rasid, Anisah
author_facet	Abd Rasid, Anisah
author_sort	Abd Rasid, Anisah
title	Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid
title_short	Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid
title_full	Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid
title_fullStr	Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid
title_full_unstemmed	Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid
title_sort	radioprotective properties of 50% watermelon juice against low dose ionizing radiation (ldir)-induced oxidative stress in mice lung / anisah abd rasid
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/22292/ http://ir.uitm.edu.my/id/eprint/22292/1/TD_ANISAH%20ABD%20RASID%20HS%20B%2015_5.pdf
first_indexed	2023-09-18T23:08:22Z
last_indexed	2023-09-18T23:08:22Z
_version_	1777418635432689664

Radioprotective properties of 50% watermelon juice against Low Dose Ionizing Radiation (LDIR)-Induced Oxidative stress in mice lung / Anisah Abd Rasid

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