Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf

Oil palm (Elaeis guineensis) is an important crop for edible oil production. Oil palm plantation is threatened by the basal stem rot disease caused by Ganoderma boninense. Several studies showed that lignin is important in plant defense. Cinnamyl Alcohol Dehydrogenase (CAD) played a crucial role in...

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Main Author:	Abdul Fataf, Mohammad Arif
Format:	Student Project
Published:	Faculty of Plantation and Agrotechnology 2018
Subjects:	S Agriculture (General) Agricultural conservation Pests and diseases
Online Access:	http://ir.uitm.edu.my/id/eprint/22633/

id	uitm-22633
recordtype	eprints
spelling	uitm-226332019-04-07T02:14:19Z http://ir.uitm.edu.my/id/eprint/22633/ Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf Abdul Fataf, Mohammad Arif S Agriculture (General) Agricultural conservation Pests and diseases Oil palm (Elaeis guineensis) is an important crop for edible oil production. Oil palm plantation is threatened by the basal stem rot disease caused by Ganoderma boninense. Several studies showed that lignin is important in plant defense. Cinnamyl Alcohol Dehydrogenase (CAD) played a crucial role in the final step of lignin biosynthetic pathway. Previous studies reported that CAD was involved in lignin biosynthesis and was important in plant defense. To date, there is no report on the study of CAD genes from oil palm. Therefore, this study was conducted to disclose the function of a bona fide CAD gene, namely EgCAD2, from oil palm. Transgenic tobacco plants overexpressing the EgCAD2 developed in previous study were cultivated in vitro for 8 weeks. Morphological analyses were performed to investigate the effects of EgCAD2 overexpression on plant growth and development. Histological analysis was carried out to examine the accumulation of lignin in the stem of transgenic plants. Overexpression of EgCAD2 resulted in alteration in plant morphology. All transgenic tobacco plants showed reduced plant height and internode length. The biomass production also decreased in transgenic plants. The transgenic plants also showed ectopic lignin accumulation in stems compared to wild-type plant. Overall, the results indicated that EgCAD2 plays a role in lignin biosynthesis and involved in plant growth and development. The functions of EgCAD2 gene was identified through transgenic tobacco plants in this study. Faculty of Plantation and Agrotechnology 2018 Student Project NonPeerReviewed Abdul Fataf, Mohammad Arif (2018) Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
topic	S Agriculture (General) Agricultural conservation Pests and diseases
spellingShingle	S Agriculture (General) Agricultural conservation Pests and diseases Abdul Fataf, Mohammad Arif Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf
description	Oil palm (Elaeis guineensis) is an important crop for edible oil production. Oil palm plantation is threatened by the basal stem rot disease caused by Ganoderma boninense. Several studies showed that lignin is important in plant defense. Cinnamyl Alcohol Dehydrogenase (CAD) played a crucial role in the final step of lignin biosynthetic pathway. Previous studies reported that CAD was involved in lignin biosynthesis and was important in plant defense. To date, there is no report on the study of CAD genes from oil palm. Therefore, this study was conducted to disclose the function of a bona fide CAD gene, namely EgCAD2, from oil palm. Transgenic tobacco plants overexpressing the EgCAD2 developed in previous study were cultivated in vitro for 8 weeks. Morphological analyses were performed to investigate the effects of EgCAD2 overexpression on plant growth and development. Histological analysis was carried out to examine the accumulation of lignin in the stem of transgenic plants. Overexpression of EgCAD2 resulted in alteration in plant morphology. All transgenic tobacco plants showed reduced plant height and internode length. The biomass production also decreased in transgenic plants. The transgenic plants also showed ectopic lignin accumulation in stems compared to wild-type plant. Overall, the results indicated that EgCAD2 plays a role in lignin biosynthesis and involved in plant growth and development. The functions of EgCAD2 gene was identified through transgenic tobacco plants in this study.
format	Student Project
author	Abdul Fataf, Mohammad Arif
author_facet	Abdul Fataf, Mohammad Arif
author_sort	Abdul Fataf, Mohammad Arif
title	Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf
title_short	Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf
title_full	Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf
title_fullStr	Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf
title_full_unstemmed	Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf
title_sort	morphological analysis of transgenic tobacco plants overexpressing the cinnamyl alcohol dehydrogenase 2 gene of oil palm / mohammad arif abdul fataf
publisher	Faculty of Plantation and Agrotechnology
publishDate	2018
url	http://ir.uitm.edu.my/id/eprint/22633/
first_indexed	2023-09-18T23:09:05Z
last_indexed	2023-09-18T23:09:05Z
_version_	1777418680955568128

Morphological analysis of transgenic tobacco plants overexpressing the Cinnamyl Alcohol Dehydrogenase 2 gene of oil palm / Mohammad Arif Abdul Fataf

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