Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz

Lignin is a phenolic heteropolymer in secondary cell walls that plays a main role in the development of plants and defence against any pathogens. The biosynthesis of monolignols, which represents the important component of lignin involves large amount enzymes. The Cinnamyl Alcohol Dehydrogenase (CAD...

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Main Author:	Abdul Aziz, Nur Afiqah
Format:	Student Project
Published:	Faculty of Plantation and Agrotechnology 2018
Subjects:	S Agriculture (General) Agriculture and the environment
Online Access:	http://ir.uitm.edu.my/id/eprint/22769/

id	uitm-22769
recordtype	eprints
spelling	uitm-227692019-05-10T08:17:15Z http://ir.uitm.edu.my/id/eprint/22769/ Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz Abdul Aziz, Nur Afiqah S Agriculture (General) Agriculture and the environment Lignin is a phenolic heteropolymer in secondary cell walls that plays a main role in the development of plants and defence against any pathogens. The biosynthesis of monolignols, which represents the important component of lignin involves large amount enzymes. The Cinnamyl Alcohol Dehydrogenase (CAD) is a key enzyme in biosynthesis of lignin that catalyses the final step in the biosynthesis of monolignols. Based on previous sequence analysis, EgCAD1 is a bona fide CAD gene involves in lignin biosynthesis in oil palm. However, it is not supported by any scientific evidences. Functional study of the gene is required to prove the statement. Therefore, this study was performed to develop transgenic plants carrying the EgCAD1 gene from oil palm in order to provide materials for future functional study of the gene. Agrobacterium-mediated transformation method was applied in this research to transform the tobacco leaf discs. Shoots were regenerated from the Agrobacterium-infected leaf discs. Subsequently, the shoots were subcultured on rooting medium to induce root formation. Multiple lines of transgenic tobacco plants were produced through this study. The transgenic plants developed in this study will permit one to identify the functions of EgCAD1 gene in future. Faculty of Plantation and Agrotechnology 2018 Student Project NonPeerReviewed Abdul Aziz, Nur Afiqah (2018) Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
topic	S Agriculture (General) Agriculture and the environment
spellingShingle	S Agriculture (General) Agriculture and the environment Abdul Aziz, Nur Afiqah Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
description	Lignin is a phenolic heteropolymer in secondary cell walls that plays a main role in the development of plants and defence against any pathogens. The biosynthesis of monolignols, which represents the important component of lignin involves large amount enzymes. The Cinnamyl Alcohol Dehydrogenase (CAD) is a key enzyme in biosynthesis of lignin that catalyses the final step in the biosynthesis of monolignols. Based on previous sequence analysis, EgCAD1 is a bona fide CAD gene involves in lignin biosynthesis in oil palm. However, it is not supported by any scientific evidences. Functional study of the gene is required to prove the statement. Therefore, this study was performed to develop transgenic plants carrying the EgCAD1 gene from oil palm in order to provide materials for future functional study of the gene. Agrobacterium-mediated transformation method was applied in this research to transform the tobacco leaf discs. Shoots were regenerated from the Agrobacterium-infected leaf discs. Subsequently, the shoots were subcultured on rooting medium to induce root formation. Multiple lines of transgenic tobacco plants were produced through this study. The transgenic plants developed in this study will permit one to identify the functions of EgCAD1 gene in future.
format	Student Project
author	Abdul Aziz, Nur Afiqah
author_facet	Abdul Aziz, Nur Afiqah
author_sort	Abdul Aziz, Nur Afiqah
title	Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_short	Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_full	Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_fullStr	Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_full_unstemmed	Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_sort	development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / nur afiqah abdul aziz
publisher	Faculty of Plantation and Agrotechnology
publishDate	2018
url	http://ir.uitm.edu.my/id/eprint/22769/
first_indexed	2023-09-18T23:09:22Z
last_indexed	2023-09-18T23:09:22Z
_version_	1777418698539139072

Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz

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