Isolation, characterization and determination of cell concentration in silicate producing bacteria / Ainul Asywanie Mislan
Silicate producing bacteria play an efficient role in soil by solubilizing the insoluble forms of minerals like magnesium trisilicate that exist in earth's crust. A total of five different bacteria were obtained and one of the isolated bacteria from bauxite soil sample known as the BI bacter...
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| Format: | Student Project |
| Language: | English |
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Faculty of Applied Sciences
2017
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| Online Access: | http://ir.uitm.edu.my/id/eprint/22976/ http://ir.uitm.edu.my/id/eprint/22976/1/PPb_AINUL%20ASYWANIE%20MISLAN%20AS%20C%2017_5.PDF |
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uitm-22976 |
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eprints |
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uitm-229762019-01-29T01:44:19Z http://ir.uitm.edu.my/id/eprint/22976/ Isolation, characterization and determination of cell concentration in silicate producing bacteria / Ainul Asywanie Mislan Mislan, Ainul Asywanie Bacteria Silicate producing bacteria play an efficient role in soil by solubilizing the insoluble forms of minerals like magnesium trisilicate that exist in earth's crust. A total of five different bacteria were obtained and one of the isolated bacteria from bauxite soil sample known as the BI bacteria was characterized by morphological observation, biochemical identification, screened for the solubilization of silicate on silicate medium besides the effect of cell concentration in enhancing the silicate absorption also being evaluated. All the five bacterial isolates were capable in solubilizing the insoluble minerals in medium. The most clear solubilization zone was observed in BI and ST5 including the positive control (Bacillus sp.). The results show that BI and ST5 give clear zone of solubilisation. BI is Gram positive, aerobic, rod shape with endospore and capsule. ST5 is Gram negative, facultative anaerobic, rod in shape with endospore. Both are acid production and both exhibit the similar colony characteristics to silicate group bacteria. The biochemical tests indicate that the bacterial isolates are similar to genus Bacillus. The capability of bacterial isolates (BI and ST5) to have absorbed silica to its cell wall by different concentration of bacterial cell which ranged from the optical density of 0.5, 1.0 and 1.5 shows there are no significance effect of different cell concentration at p ~ 0.05 neither on silicate absorption nor weight of pelleted cell. BI with the absorption of silicate up to 44.5 mM is the most prominent candidate to be silicate producing bacteria. The capability of silicate bacteria to consolidate sand can be use in ecological engineering purpose in the future and leading to biomineralization. Faculty of Applied Sciences 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/22976/1/PPb_AINUL%20ASYWANIE%20MISLAN%20AS%20C%2017_5.PDF Mislan, Ainul Asywanie (2017) Isolation, characterization and determination of cell concentration in silicate producing bacteria / Ainul Asywanie Mislan. [Student Project] (Unpublished) |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Teknologi MARA |
| building |
UiTM Institutional Repository |
| collection |
Online Access |
| language |
English |
| topic |
Bacteria |
| spellingShingle |
Bacteria Mislan, Ainul Asywanie Isolation, characterization and determination of cell concentration in silicate producing bacteria / Ainul Asywanie Mislan |
| description |
Silicate producing bacteria play an efficient role in soil by solubilizing the insoluble
forms of minerals like magnesium trisilicate that exist in earth's crust. A total of five
different bacteria were obtained and one of the isolated bacteria from bauxite soil
sample known as the BI bacteria was characterized by morphological observation,
biochemical identification, screened for the solubilization of silicate on silicate
medium besides the effect of cell concentration in enhancing the silicate absorption
also being evaluated. All the five bacterial isolates were capable in solubilizing the
insoluble minerals in medium. The most clear solubilization zone was observed in BI
and ST5 including the positive control (Bacillus sp.). The results show that BI and
ST5 give clear zone of solubilisation. BI is Gram positive, aerobic, rod shape with
endospore and capsule. ST5 is Gram negative, facultative anaerobic, rod in shape
with endospore. Both are acid production and both exhibit the similar colony
characteristics to silicate group bacteria. The biochemical tests indicate that the
bacterial isolates are similar to genus Bacillus. The capability of bacterial isolates (BI
and ST5) to have absorbed silica to its cell wall by different concentration of bacterial
cell which ranged from the optical density of 0.5, 1.0 and 1.5 shows there are no
significance effect of different cell concentration at p ~ 0.05 neither on silicate
absorption nor weight of pelleted cell. BI with the absorption of silicate up to 44.5
mM is the most prominent candidate to be silicate producing bacteria. The capability
of silicate bacteria to consolidate sand can be use in ecological engineering purpose in
the future and leading to biomineralization. |
| format |
Student Project |
| author |
Mislan, Ainul Asywanie |
| author_facet |
Mislan, Ainul Asywanie |
| author_sort |
Mislan, Ainul Asywanie |
| title |
Isolation, characterization and determination of
cell concentration in silicate producing bacteria / Ainul Asywanie Mislan |
| title_short |
Isolation, characterization and determination of
cell concentration in silicate producing bacteria / Ainul Asywanie Mislan |
| title_full |
Isolation, characterization and determination of
cell concentration in silicate producing bacteria / Ainul Asywanie Mislan |
| title_fullStr |
Isolation, characterization and determination of
cell concentration in silicate producing bacteria / Ainul Asywanie Mislan |
| title_full_unstemmed |
Isolation, characterization and determination of
cell concentration in silicate producing bacteria / Ainul Asywanie Mislan |
| title_sort |
isolation, characterization and determination of
cell concentration in silicate producing bacteria / ainul asywanie mislan |
| publisher |
Faculty of Applied Sciences |
| publishDate |
2017 |
| url |
http://ir.uitm.edu.my/id/eprint/22976/ http://ir.uitm.edu.my/id/eprint/22976/1/PPb_AINUL%20ASYWANIE%20MISLAN%20AS%20C%2017_5.PDF |
| first_indexed |
2023-09-18T23:09:49Z |
| last_indexed |
2023-09-18T23:09:49Z |
| _version_ |
1777418726822379520 |