Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh

Antioxidant is defined as the substance that present at low concentration together with oxidizable substrate to delay or prevent the substrate to be oxidized. Swietenia macrophylla is a plant known to cure high blood pressure and also exhibit antioxidant properties. This study was conducted to evalu...

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Main Author:	Mohd Raoh, Mardhiah
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2014
Subjects:	QR Microbiology Bacteria
Online Access:	http://ir.uitm.edu.my/id/eprint/23565/ http://ir.uitm.edu.my/id/eprint/23565/1/PPb_MARDHIAH%20MOHD%20RAOH%20AS%20N%2014_5.pdf

id	uitm-23565
recordtype	eprints
spelling	uitm-235652019-04-10T08:22:19Z http://ir.uitm.edu.my/id/eprint/23565/ Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh Mohd Raoh, Mardhiah QR Microbiology Bacteria Antioxidant is defined as the substance that present at low concentration together with oxidizable substrate to delay or prevent the substrate to be oxidized. Swietenia macrophylla is a plant known to cure high blood pressure and also exhibit antioxidant properties. This study was conducted to evaluate the effects of extraction solvents on antioxidant activity of Swietenia macrophylla extracts. Solvents used in this study were distilled water, 70% ethanol and 70% methanol. Ground Swietenia macrophylla leaves were soaked with three different types of solvents with ratio 10 ml solvent : 1 g sample for 2 days. The extracts later were dried using rotary evaporator at 40˚C. The crude extracts were tested in total phenolic content (TPC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay to evaluate the antioxidant activity. IC50 value is the oxidation index used to measure the 50% depletion of DPPH radical scavenging activity. Lower IC50 value indicate higher antioxidant activity. Extraction from 70% ethanol showed the lowest IC50 value of 0.927 mg/ml followed by 70% methanol (0.968 mg/ml) and distilled water (2.278 mg/ml) when compared to BHT (0.811 mg/ml) and ascorbic acid (0.336 mg/ml) as the positive control and reference compound. Extraction from 70% ethanol showed the highest total phenolic content of 0.4467 ± 0.32 mgGAE/g of dry weight, followed by 70% methanol (0.3795 ± 0.01 mgGAE/g) and distilled water (0.1757 ± 0.04 mgGAE/g). However, three extraction solvents used in this study showed no significance difference in term of total phenolic content. Faculty of Applied Sciences 2014 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/23565/1/PPb_MARDHIAH%20MOHD%20RAOH%20AS%20N%2014_5.pdf Mohd Raoh, Mardhiah (2014) Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology Bacteria
spellingShingle	QR Microbiology Bacteria Mohd Raoh, Mardhiah Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh
description	Antioxidant is defined as the substance that present at low concentration together with oxidizable substrate to delay or prevent the substrate to be oxidized. Swietenia macrophylla is a plant known to cure high blood pressure and also exhibit antioxidant properties. This study was conducted to evaluate the effects of extraction solvents on antioxidant activity of Swietenia macrophylla extracts. Solvents used in this study were distilled water, 70% ethanol and 70% methanol. Ground Swietenia macrophylla leaves were soaked with three different types of solvents with ratio 10 ml solvent : 1 g sample for 2 days. The extracts later were dried using rotary evaporator at 40˚C. The crude extracts were tested in total phenolic content (TPC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay to evaluate the antioxidant activity. IC50 value is the oxidation index used to measure the 50% depletion of DPPH radical scavenging activity. Lower IC50 value indicate higher antioxidant activity. Extraction from 70% ethanol showed the lowest IC50 value of 0.927 mg/ml followed by 70% methanol (0.968 mg/ml) and distilled water (2.278 mg/ml) when compared to BHT (0.811 mg/ml) and ascorbic acid (0.336 mg/ml) as the positive control and reference compound. Extraction from 70% ethanol showed the highest total phenolic content of 0.4467 ± 0.32 mgGAE/g of dry weight, followed by 70% methanol (0.3795 ± 0.01 mgGAE/g) and distilled water (0.1757 ± 0.04 mgGAE/g). However, three extraction solvents used in this study showed no significance difference in term of total phenolic content.
format	Student Project
author	Mohd Raoh, Mardhiah
author_facet	Mohd Raoh, Mardhiah
author_sort	Mohd Raoh, Mardhiah
title	Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh
title_short	Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh
title_full	Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh
title_fullStr	Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh
title_full_unstemmed	Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh
title_sort	effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / mardhiah mohd raoh
publisher	Faculty of Applied Sciences
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/23565/ http://ir.uitm.edu.my/id/eprint/23565/1/PPb_MARDHIAH%20MOHD%20RAOH%20AS%20N%2014_5.pdf
first_indexed	2023-09-18T23:10:56Z
last_indexed	2023-09-18T23:10:56Z
_version_	1777418797826703360

Effect of extraction solvents on antioxidant activity of swietenia macrophylla extracts / Mardhiah Mohd Raoh

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