Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad

Fusarium wilt is one of the diseases that often-attacked plants in Malaysia such as bananas, tomatoes, black pepper, rice and others. The various methods have been made to control Fusarium wilt diseases including the use of chemicals. However, the extensive use of chemicals will become a negatively...

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Main Author:	Ahmad, Mohd Rasyid
Format:	Student Project
Published:	Faculty of Plantation and Agrotechnology 2019
Subjects:	Agricultural industries QK Botany
Online Access:	http://ir.uitm.edu.my/id/eprint/24157/

id	uitm-24157
recordtype	eprints
spelling	uitm-241572019-05-16T02:31:19Z http://ir.uitm.edu.my/id/eprint/24157/ Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad Ahmad, Mohd Rasyid Agricultural industries QK Botany Fusarium wilt is one of the diseases that often-attacked plants in Malaysia such as bananas, tomatoes, black pepper, rice and others. The various methods have been made to control Fusarium wilt diseases including the use of chemicals. However, the extensive use of chemicals will become a negatively impacts to environment and plants are more susceptible to pests and diseases. Hence, this study was used wasted product with plant extract method from part of Elaeis guineensis such as empty fruit bunches (EFB), pressed palm fibres (PPF), kernel, leaf and trunk by using methanol solvent to control Fusarium oxysporum. TLC profiling was used to identify the best solvent to separate the pure compound. While the phytochemical screening was analyzed for the presences of alkaloid, flavonoid, terpenoid, tannins and saponins. All the extract was evaluated their antimicrobial properties with 25 and 50 ppm based on disk diffusion method. Trichoderma sp. as a positive standard control against Fusarium oxysporum. The results of phytochemical screening process confirmed the presence of alkaloids, flavonoids, tannins and terpenoid in Elaies guineensis extract. Hexane: Ethyl acetate, 2:8 is the best solvent system which it possed in several compound especially in kernel, leaf and PPF with 6,3 and 2 compounds. Antifungal activity revealed that trunk extract to control Fusarium oxysporum with moderate inhibitory zone 10.67mm in 25mg/mL and 14.33mm in 50mg/mL followed by leaf with slightly inhibitory zone 9.67mm in 25mg/mL and 13.00mm moderate inhibitory zone in 50mg/mL. These finding provided the evidence that wasted product from part of Elaies guineensis had a potential for replacement of Trichoderma sp. against Fusarium oxysporum. Faculty of Plantation and Agrotechnology 2019 Student Project NonPeerReviewed Ahmad, Mohd Rasyid (2019) Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
topic	Agricultural industries QK Botany
spellingShingle	Agricultural industries QK Botany Ahmad, Mohd Rasyid Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad
description	Fusarium wilt is one of the diseases that often-attacked plants in Malaysia such as bananas, tomatoes, black pepper, rice and others. The various methods have been made to control Fusarium wilt diseases including the use of chemicals. However, the extensive use of chemicals will become a negatively impacts to environment and plants are more susceptible to pests and diseases. Hence, this study was used wasted product with plant extract method from part of Elaeis guineensis such as empty fruit bunches (EFB), pressed palm fibres (PPF), kernel, leaf and trunk by using methanol solvent to control Fusarium oxysporum. TLC profiling was used to identify the best solvent to separate the pure compound. While the phytochemical screening was analyzed for the presences of alkaloid, flavonoid, terpenoid, tannins and saponins. All the extract was evaluated their antimicrobial properties with 25 and 50 ppm based on disk diffusion method. Trichoderma sp. as a positive standard control against Fusarium oxysporum. The results of phytochemical screening process confirmed the presence of alkaloids, flavonoids, tannins and terpenoid in Elaies guineensis extract. Hexane: Ethyl acetate, 2:8 is the best solvent system which it possed in several compound especially in kernel, leaf and PPF with 6,3 and 2 compounds. Antifungal activity revealed that trunk extract to control Fusarium oxysporum with moderate inhibitory zone 10.67mm in 25mg/mL and 14.33mm in 50mg/mL followed by leaf with slightly inhibitory zone 9.67mm in 25mg/mL and 13.00mm moderate inhibitory zone in 50mg/mL. These finding provided the evidence that wasted product from part of Elaies guineensis had a potential for replacement of Trichoderma sp. against Fusarium oxysporum.
format	Student Project
author	Ahmad, Mohd Rasyid
author_facet	Ahmad, Mohd Rasyid
author_sort	Ahmad, Mohd Rasyid
title	Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad
title_short	Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad
title_full	Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad
title_fullStr	Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad
title_full_unstemmed	Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad
title_sort	phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / mohd rasyid ahmad
publisher	Faculty of Plantation and Agrotechnology
publishDate	2019
url	http://ir.uitm.edu.my/id/eprint/24157/
first_indexed	2023-09-18T23:12:00Z
last_indexed	2023-09-18T23:12:00Z
_version_	1777418864416522240

Phytochemical screening, tlc profiling and antifungal activity of elaies guineensis against fusarium oxysporum / Mohd Rasyid Ahmad

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