Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan

This study is about the determination of antioxidant activity in the stem of E. spiralis. Phytochemical screening test was performed by using the methanol extraction. Then, the methanol extract was proceed to determine the functional group using FTIR. Thin Layer Chromatographic (TLC) Analysis was...

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Main Author:	Mohd Azenan, Norshazleen
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2017
Subjects:	QD Chemistry Analytical chemistry
Online Access:	http://ir.uitm.edu.my/id/eprint/24164/ http://ir.uitm.edu.my/id/eprint/24164/1/PPb_NORSHAZLEEN%20MOHD%20AZENAN%20AS%20C%2017_5.PDF

id	uitm-24164
recordtype	eprints
spelling	uitm-241642019-05-10T06:53:05Z http://ir.uitm.edu.my/id/eprint/24164/ Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan Mohd Azenan, Norshazleen QD Chemistry Analytical chemistry This study is about the determination of antioxidant activity in the stem of E. spiralis. Phytochemical screening test was performed by using the methanol extraction. Then, the methanol extract was proceed to determine the functional group using FTIR. Thin Layer Chromatographic (TLC) Analysis was developed to identify the compound that presence in the stem of E. spiralis. Each TLC was sprayed by using spraying reagent to identify compound that contains in each extract. After the extraction of methanol, dichloromethane, and petroleum ether was done, the best solvent system was used to developed TLC analysis. The best solvent system for MeOH extraction is 27:3 (HEX:EA), DCM extraction is 29.4:0.6 (DCM:MeOH), and PE extraction is 24:6 (Chloroform :Acetone). Then, serial dilution was conducted to investigate the antioxidative assay in the stem of E. spiralis and also identify IC50. Methanol extract has the highest antioxidant activity. Isolation of antioxidative compound from PE extract from different Rf then proceed with GC-MS analysis. Oxiraneoctanoic acid, 3-octyl-, cis, 9,12 Octadecadienoic acid, Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester, Butylaldehyde, 4-benzyloxy-4-[2,2,-dimethyl-4-dioxolanyl], Butylaldehyde, 4-benzyloxy-4-[2,2,-dimethyl-4-dioxolanyl], Hexadecanoic acid, methyl ester, IH-Indene, I-methylene, Benzene, 1,2,4,5-tetramethyl, and trans-Verbenyl isovalerate have been isolated and identified by GC-MS. Faculty of Applied Sciences 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24164/1/PPb_NORSHAZLEEN%20MOHD%20AZENAN%20AS%20C%2017_5.PDF Mohd Azenan, Norshazleen (2017) Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry Analytical chemistry
spellingShingle	QD Chemistry Analytical chemistry Mohd Azenan, Norshazleen Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan
description	This study is about the determination of antioxidant activity in the stem of E. spiralis. Phytochemical screening test was performed by using the methanol extraction. Then, the methanol extract was proceed to determine the functional group using FTIR. Thin Layer Chromatographic (TLC) Analysis was developed to identify the compound that presence in the stem of E. spiralis. Each TLC was sprayed by using spraying reagent to identify compound that contains in each extract. After the extraction of methanol, dichloromethane, and petroleum ether was done, the best solvent system was used to developed TLC analysis. The best solvent system for MeOH extraction is 27:3 (HEX:EA), DCM extraction is 29.4:0.6 (DCM:MeOH), and PE extraction is 24:6 (Chloroform :Acetone). Then, serial dilution was conducted to investigate the antioxidative assay in the stem of E. spiralis and also identify IC50. Methanol extract has the highest antioxidant activity. Isolation of antioxidative compound from PE extract from different Rf then proceed with GC-MS analysis. Oxiraneoctanoic acid, 3-octyl-, cis, 9,12 Octadecadienoic acid, Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester, Butylaldehyde, 4-benzyloxy-4-[2,2,-dimethyl-4-dioxolanyl], Butylaldehyde, 4-benzyloxy-4-[2,2,-dimethyl-4-dioxolanyl], Hexadecanoic acid, methyl ester, IH-Indene, I-methylene, Benzene, 1,2,4,5-tetramethyl, and trans-Verbenyl isovalerate have been isolated and identified by GC-MS.
format	Student Project
author	Mohd Azenan, Norshazleen
author_facet	Mohd Azenan, Norshazleen
author_sort	Mohd Azenan, Norshazleen
title	Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan
title_short	Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan
title_full	Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan
title_fullStr	Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan
title_full_unstemmed	Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan
title_sort	investigation of antioxidative constituents from the stem of entada spiralis / norshazleen mohd azenan
publisher	Faculty of Applied Sciences
publishDate	2017
url	http://ir.uitm.edu.my/id/eprint/24164/ http://ir.uitm.edu.my/id/eprint/24164/1/PPb_NORSHAZLEEN%20MOHD%20AZENAN%20AS%20C%2017_5.PDF
first_indexed	2023-09-18T23:12:01Z
last_indexed	2023-09-18T23:12:01Z
_version_	1777418865463001088

Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan

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