Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh

Introduction: Pharmacogenomic studies contribute to genetic information in preventing severe side effects of drugs. Genetic polymorphisms in drug metabolizing enzymes such as dihydropyrimidine dehydrogenase (DPD) had been associated with variable clinical outcomes in many commonly prescribed chemoth...

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Main Authors:	Teh, Lay Kek, Salleh, Mohd Zaki
Format:	Research Reports
Language:	English
Published:	Research Management Institute (RMI) 2010
Subjects:	Cancer
Online Access:	http://ir.uitm.edu.my/id/eprint/24808/ http://ir.uitm.edu.my/id/eprint/24808/1/LP_TEH%20LAY%20KEK%20RMI%2010_5.pdf

id	uitm-24808
recordtype	eprints
spelling	uitm-248082019-07-19T03:07:20Z http://ir.uitm.edu.my/id/eprint/24808/ Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh Teh, Lay Kek Salleh, Mohd Zaki Cancer Introduction: Pharmacogenomic studies contribute to genetic information in preventing severe side effects of drugs. Genetic polymorphisms in drug metabolizing enzymes such as dihydropyrimidine dehydrogenase (DPD) had been associated with variable clinical outcomes in many commonly prescribed chemotherapy drugs including 5-Fluorouracil and irinotecan. Objectives: The review of literature had shed lights to the importance and possible impact of genetic polymorphism of DPYD and TPMT in individualization of drug therapy for 5-FU and thiopurines. However, there were no data reported for Malaysian. Current study thus aimed to explore the role of pharmacogenetics in personalized medicine in our own population. Materials and methods: Genotyping methods for DPYD and TPMT were developed using dHPLC and allele specific PCR respectively. 5-FU levels were measured in colorectal cancer patients using developed method. DNA from healthy volunteers and patients were screened. Results: Genotyping of DPYD had detected one reported mutation DPYD5, two new mutations in exon 14 1823 T>C and 1827 G>A and one intronic reagion of exon 13, 13 IVS-11G>A with allele frequencies of 14.5%, .9.1%, 9.1% and 0.9% respectively. Genotyping for TPMT revealed 7 (7%) to be heterozygous for TPMT variant alleles. The predominant allele detected is TPMT3C and is in concordance with previous studies done on Southeast Asian populations. Research Management Institute (RMI) 2010 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24808/1/LP_TEH%20LAY%20KEK%20RMI%2010_5.pdf Teh, Lay Kek and Salleh, Mohd Zaki (2010) Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh. [Research Reports] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Cancer
spellingShingle	Cancer Teh, Lay Kek Salleh, Mohd Zaki Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh
description	Introduction: Pharmacogenomic studies contribute to genetic information in preventing severe side effects of drugs. Genetic polymorphisms in drug metabolizing enzymes such as dihydropyrimidine dehydrogenase (DPD) had been associated with variable clinical outcomes in many commonly prescribed chemotherapy drugs including 5-Fluorouracil and irinotecan. Objectives: The review of literature had shed lights to the importance and possible impact of genetic polymorphism of DPYD and TPMT in individualization of drug therapy for 5-FU and thiopurines. However, there were no data reported for Malaysian. Current study thus aimed to explore the role of pharmacogenetics in personalized medicine in our own population. Materials and methods: Genotyping methods for DPYD and TPMT were developed using dHPLC and allele specific PCR respectively. 5-FU levels were measured in colorectal cancer patients using developed method. DNA from healthy volunteers and patients were screened. Results: Genotyping of DPYD had detected one reported mutation DPYD5, two new mutations in exon 14 1823 T>C and 1827 G>A and one intronic reagion of exon 13, 13 IVS-11G>A with allele frequencies of 14.5%, .9.1%, 9.1% and 0.9% respectively. Genotyping for TPMT revealed 7 (7%) to be heterozygous for TPMT variant alleles. The predominant allele detected is TPMT3C and is in concordance with previous studies done on Southeast Asian populations.
format	Research Reports
author	Teh, Lay Kek Salleh, Mohd Zaki
author_facet	Teh, Lay Kek Salleh, Mohd Zaki
author_sort	Teh, Lay Kek
title	Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh
title_short	Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh
title_full	Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh
title_fullStr	Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh
title_full_unstemmed	Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh
title_sort	cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / prof madya dr teh lay kek and prof dr mohd zaki salleh
publisher	Research Management Institute (RMI)
publishDate	2010
url	http://ir.uitm.edu.my/id/eprint/24808/ http://ir.uitm.edu.my/id/eprint/24808/1/LP_TEH%20LAY%20KEK%20RMI%2010_5.pdf
first_indexed	2023-09-18T23:13:24Z
last_indexed	2023-09-18T23:13:24Z
_version_	1777418952551432192

Cancer pharmacogenomics: the relevance of genetic profile in optimization drug therapy for azathiopurine and 5-fluorouracil / Prof Madya Dr Teh Lay Kek and Prof Dr Mohd Zaki Salleh

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