Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid

Chromolaena odorata belongs to the Asteraceae family which was originally came from Central and South America. In this study the efficiency of a modified CTAB method for DNA extraction from local C. odorata leaves were determined based on their DNA concentration, DNA yield, DNA band and purity. The...

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Main Author:	Md Yazid, Nur Hidayah
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2014
Subjects:	Extraction (Chemistry) QR Microbiology
Online Access:	http://ir.uitm.edu.my/id/eprint/24870/ http://ir.uitm.edu.my/id/eprint/24870/1/PPb_NUR%20HIDAYAH%20MD%20YAZID%20AS%20N%2014_5.pdf

id	uitm-24870
recordtype	eprints
spelling	uitm-248702019-07-22T02:24:23Z http://ir.uitm.edu.my/id/eprint/24870/ Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid Md Yazid, Nur Hidayah Extraction (Chemistry) QR Microbiology Chromolaena odorata belongs to the Asteraceae family which was originally came from Central and South America. In this study the efficiency of a modified CTAB method for DNA extraction from local C. odorata leaves were determined based on their DNA concentration, DNA yield, DNA band and purity. The optimum PCR annealing temperature of local C. odorata was identified by using ITS1 and ITS4 primer amplification. More clear and intact DNA band was produced from extraction using modified CTAB method. The values obtained from the CTAB extraction method for DNA purity, DNA concentration and DNA yield were 1.65±0.06, 101.6+7.638 ng/ uL and 152.5±11.456 ng/mL respectively. The values obtained from the modified CTAB method for DNA purity, DNA concentration and DNA yield were 1.82±0.03, 118.3+7.638 ng/ uL and 177.5+11.456 ng/mL respectively. Modified CTAB extraction method are much more preferable to isolate DNA from local C. odorata due to the presence of reagents such as phenol, polyvinylpyrrolidone, B-mercaptoethanol and also extended treatment of RNase. This study showed that the exact annealing temperature for the isolated DNA from C.odorata with ITS 1 and ITS4 amplification were 65°C and 63°C respectively. Both PCR products obtained were in the range of 250 to 300 base pair in size, which indicates that the amplicon size referred to the primer characteristics. Faculty of Applied Sciences 2014 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24870/1/PPb_NUR%20HIDAYAH%20MD%20YAZID%20AS%20N%2014_5.pdf Md Yazid, Nur Hidayah (2014) Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Extraction (Chemistry) QR Microbiology
spellingShingle	Extraction (Chemistry) QR Microbiology Md Yazid, Nur Hidayah Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid
description	Chromolaena odorata belongs to the Asteraceae family which was originally came from Central and South America. In this study the efficiency of a modified CTAB method for DNA extraction from local C. odorata leaves were determined based on their DNA concentration, DNA yield, DNA band and purity. The optimum PCR annealing temperature of local C. odorata was identified by using ITS1 and ITS4 primer amplification. More clear and intact DNA band was produced from extraction using modified CTAB method. The values obtained from the CTAB extraction method for DNA purity, DNA concentration and DNA yield were 1.65±0.06, 101.6+7.638 ng/ uL and 152.5±11.456 ng/mL respectively. The values obtained from the modified CTAB method for DNA purity, DNA concentration and DNA yield were 1.82±0.03, 118.3+7.638 ng/ uL and 177.5+11.456 ng/mL respectively. Modified CTAB extraction method are much more preferable to isolate DNA from local C. odorata due to the presence of reagents such as phenol, polyvinylpyrrolidone, B-mercaptoethanol and also extended treatment of RNase. This study showed that the exact annealing temperature for the isolated DNA from C.odorata with ITS 1 and ITS4 amplification were 65°C and 63°C respectively. Both PCR products obtained were in the range of 250 to 300 base pair in size, which indicates that the amplicon size referred to the primer characteristics.
format	Student Project
author	Md Yazid, Nur Hidayah
author_facet	Md Yazid, Nur Hidayah
author_sort	Md Yazid, Nur Hidayah
title	Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid
title_short	Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid
title_full	Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid
title_fullStr	Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid
title_full_unstemmed	Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid
title_sort	optimization of annealing temperature from local chromolaena odorata / nur hidayah md yazid
publisher	Faculty of Applied Sciences
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/24870/ http://ir.uitm.edu.my/id/eprint/24870/1/PPb_NUR%20HIDAYAH%20MD%20YAZID%20AS%20N%2014_5.pdf
first_indexed	2023-09-18T23:13:33Z
last_indexed	2023-09-18T23:13:33Z
_version_	1777418961469571072

Optimization of annealing temperature from local chromolaena odorata / Nur Hidayah Md Yazid

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