Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar

Rice (Oryza sativa L.) is an important food crop and was consumed approximately 40% of people all over the world. In Malaysia approximately 1.95 million tonne of rice was produced and it is believed to increase within the growing population. Simple Sequence Repeats (SSRs) is one of the molecular mar...

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Main Author:	Mokhtar, Noor Suhada
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2015
Subjects:	QR Microbiology
Online Access:	http://ir.uitm.edu.my/id/eprint/24930/ http://ir.uitm.edu.my/id/eprint/24930/1/PPb_NOOR%20SUHADA%20MOKHTAR%20AS%20N%2015_5.pdf

id	uitm-24930
recordtype	eprints
spelling	uitm-249302019-07-25T03:34:30Z http://ir.uitm.edu.my/id/eprint/24930/ Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar Mokhtar, Noor Suhada QR Microbiology Rice (Oryza sativa L.) is an important food crop and was consumed approximately 40% of people all over the world. In Malaysia approximately 1.95 million tonne of rice was produced and it is believed to increase within the growing population. Simple Sequence Repeats (SSRs) is one of the molecular markers that had been widely used to assist breeding approach in analyzing genetic relatedness, identification and selection of desired traits. A study was conducted to identify the polymorphism analysis between two local Malaysian rice varieties which are MR 264 and Pongsu Seribu. The extractions DNA were prepared through maceration of leaves using the modified CTAB (Cetyltrimethylammonium bromide) method. In this study, the percent of seeds survived were 88.2%. Polymorphism analysis was assayed by fifty SSR marker and fourteen marker were showed amplified bands are namely RM 495, RM1167, RM 148, RM 168, RM 146, RM 144, RM 101, RM 206, RM 1233, RM 8225, RM 125, RM 248, RM 304 and RM 333. Out of fourteen, only RM 495 and RM 1167 were demonstrated the polymorphic bands electrophoresed in 4% agarose gel which were mapped on chromosome number one. Faculty of Applied Sciences 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24930/1/PPb_NOOR%20SUHADA%20MOKHTAR%20AS%20N%2015_5.pdf Mokhtar, Noor Suhada (2015) Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Mokhtar, Noor Suhada Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar
description	Rice (Oryza sativa L.) is an important food crop and was consumed approximately 40% of people all over the world. In Malaysia approximately 1.95 million tonne of rice was produced and it is believed to increase within the growing population. Simple Sequence Repeats (SSRs) is one of the molecular markers that had been widely used to assist breeding approach in analyzing genetic relatedness, identification and selection of desired traits. A study was conducted to identify the polymorphism analysis between two local Malaysian rice varieties which are MR 264 and Pongsu Seribu. The extractions DNA were prepared through maceration of leaves using the modified CTAB (Cetyltrimethylammonium bromide) method. In this study, the percent of seeds survived were 88.2%. Polymorphism analysis was assayed by fifty SSR marker and fourteen marker were showed amplified bands are namely RM 495, RM1167, RM 148, RM 168, RM 146, RM 144, RM 101, RM 206, RM 1233, RM 8225, RM 125, RM 248, RM 304 and RM 333. Out of fourteen, only RM 495 and RM 1167 were demonstrated the polymorphic bands electrophoresed in 4% agarose gel which were mapped on chromosome number one.
format	Student Project
author	Mokhtar, Noor Suhada
author_facet	Mokhtar, Noor Suhada
author_sort	Mokhtar, Noor Suhada
title	Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar
title_short	Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar
title_full	Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar
title_fullStr	Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar
title_full_unstemmed	Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar
title_sort	polymorphism analysis between two local malaysian rice varieties / noor suhada mokhtar
publisher	Faculty of Applied Sciences
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/24930/ http://ir.uitm.edu.my/id/eprint/24930/1/PPb_NOOR%20SUHADA%20MOKHTAR%20AS%20N%2015_5.pdf
first_indexed	2023-09-18T23:13:41Z
last_indexed	2023-09-18T23:13:41Z
_version_	1777418969882296320

Polymorphism analysis between two local Malaysian rice varieties / Noor Suhada Mokhtar

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