Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah

Lactobacilli are gram positive, facultative bacteria which convert lactose and simple sugars to lactic acid. Species belonging to the genus Lactobacillus are used specifically in dairy products. The main purpose of this study was to isolate the Lactobacillus sp. from the dairy product samples and to...

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Bibliographic Details
Main Author:	Iskandarshah, Nurul Fatin
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2015
Subjects:	Extraction (Chemistry) Bacteria
Online Access:	http://ir.uitm.edu.my/id/eprint/25132/ http://ir.uitm.edu.my/id/eprint/25132/1/PPb_NURUL%20FATIN%20ISKANDARSHAH%20AS%20N%2015_5.pdf

id	uitm-25132
recordtype	eprints
spelling	uitm-251322019-08-09T02:41:16Z http://ir.uitm.edu.my/id/eprint/25132/ Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah Iskandarshah, Nurul Fatin Extraction (Chemistry) Bacteria Lactobacilli are gram positive, facultative bacteria which convert lactose and simple sugars to lactic acid. Species belonging to the genus Lactobacillus are used specifically in dairy products. The main purpose of this study was to isolate the Lactobacillus sp. from the dairy product samples and to identify Lactobacillus sp. in dairy product samples by using PCR method. A total of four samples from dairy products which are yogurt, vitagen, yakult, goat milk and three Lactobacillus sp. strains were used in this study. Dairy product samples were purchased at Econsave supermarket and Lactobacillus strains were obtained from Makmal Halal UNISEL, Selangor. Streak plate method was carried out on MRS agar plates to isolate Lactobacillus sp. from the samples. The genomic DNA from these samples were extracted using RTP® Bacteria DNA Mini Kit and were subjected to PCR amplification for the detection of Lactobacillus species using specific primers. All samples showed positive result of DNA band on agarose gel electrophoresis. The extraction DNA method using RTP® Bacteria DNA Mini Kit is suggested for high quality and more reliable result. Faculty of Applied Sciences 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/25132/1/PPb_NURUL%20FATIN%20ISKANDARSHAH%20AS%20N%2015_5.pdf Iskandarshah, Nurul Fatin (2015) Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Extraction (Chemistry) Bacteria
spellingShingle	Extraction (Chemistry) Bacteria Iskandarshah, Nurul Fatin Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
description	Lactobacilli are gram positive, facultative bacteria which convert lactose and simple sugars to lactic acid. Species belonging to the genus Lactobacillus are used specifically in dairy products. The main purpose of this study was to isolate the Lactobacillus sp. from the dairy product samples and to identify Lactobacillus sp. in dairy product samples by using PCR method. A total of four samples from dairy products which are yogurt, vitagen, yakult, goat milk and three Lactobacillus sp. strains were used in this study. Dairy product samples were purchased at Econsave supermarket and Lactobacillus strains were obtained from Makmal Halal UNISEL, Selangor. Streak plate method was carried out on MRS agar plates to isolate Lactobacillus sp. from the samples. The genomic DNA from these samples were extracted using RTP® Bacteria DNA Mini Kit and were subjected to PCR amplification for the detection of Lactobacillus species using specific primers. All samples showed positive result of DNA band on agarose gel electrophoresis. The extraction DNA method using RTP® Bacteria DNA Mini Kit is suggested for high quality and more reliable result.
format	Student Project
author	Iskandarshah, Nurul Fatin
author_facet	Iskandarshah, Nurul Fatin
author_sort	Iskandarshah, Nurul Fatin
title	Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_short	Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_full	Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_fullStr	Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_full_unstemmed	Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_sort	isolation and identification of lactobacillus sp. from dairy products using pcr method / nurul fatin iskandarshah
publisher	Faculty of Applied Sciences
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/25132/ http://ir.uitm.edu.my/id/eprint/25132/1/PPb_NURUL%20FATIN%20ISKANDARSHAH%20AS%20N%2015_5.pdf
first_indexed	2023-09-18T23:14:07Z
last_indexed	2023-09-18T23:14:07Z
_version_	1777418997576237056

Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah

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