Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar

Curculigo latifolia (lemba) is an underutilized plant with high value especially medicinal properties. Even though traditionally used for food and natural remedies, it still possessed low market value due to lack of effort in cultivating the sources. Leaf explants were inoculated onto MS basal mediu...

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Main Author:	Basar, Nurul Syafira
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2015
Subjects:	QR Microbiology
Online Access:	http://ir.uitm.edu.my/id/eprint/25133/ http://ir.uitm.edu.my/id/eprint/25133/1/PPb_NURUL%20SYAFIRA%20BASAR%20AS%20N%2015_5.pdf

id	uitm-25133
recordtype	eprints
spelling	uitm-251332019-08-09T02:38:44Z http://ir.uitm.edu.my/id/eprint/25133/ Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar Basar, Nurul Syafira QR Microbiology Curculigo latifolia (lemba) is an underutilized plant with high value especially medicinal properties. Even though traditionally used for food and natural remedies, it still possessed low market value due to lack of effort in cultivating the sources. Leaf explants were inoculated onto MS basal medium supplemented with combination of 6- Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) which vary in concentrations. In this study, the ranges on which the different hormones treatments that able to induce the response on C. latifolia was observed and determined. Direct root organogenesis were formed from leaf explant grown on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg/L NAA alone and combination of 3.0 mg/L NAA and 0.5 mg/L BAP after four and six week, respectively. The highest number of root per explant were obtained in MS medium supplemented with NAA alone with average 5 roots emerged from one explant. Technique of sterilization was improved when disinfect the explant using 1 % HgCl2. Even though aseptic condition and sterilization has been employed, contamination of cultures was still the most pressing problem in this study, whereby the contaminations were not successfully eliminated. Necrosis in culture material indicated the release of phenolic compounds. Further research should be concentrated on completely eliminate the contamination in culture. Faculty of Applied Sciences 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/25133/1/PPb_NURUL%20SYAFIRA%20BASAR%20AS%20N%2015_5.pdf Basar, Nurul Syafira (2015) Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Basar, Nurul Syafira Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar
description	Curculigo latifolia (lemba) is an underutilized plant with high value especially medicinal properties. Even though traditionally used for food and natural remedies, it still possessed low market value due to lack of effort in cultivating the sources. Leaf explants were inoculated onto MS basal medium supplemented with combination of 6- Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) which vary in concentrations. In this study, the ranges on which the different hormones treatments that able to induce the response on C. latifolia was observed and determined. Direct root organogenesis were formed from leaf explant grown on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg/L NAA alone and combination of 3.0 mg/L NAA and 0.5 mg/L BAP after four and six week, respectively. The highest number of root per explant were obtained in MS medium supplemented with NAA alone with average 5 roots emerged from one explant. Technique of sterilization was improved when disinfect the explant using 1 % HgCl2. Even though aseptic condition and sterilization has been employed, contamination of cultures was still the most pressing problem in this study, whereby the contaminations were not successfully eliminated. Necrosis in culture material indicated the release of phenolic compounds. Further research should be concentrated on completely eliminate the contamination in culture.
format	Student Project
author	Basar, Nurul Syafira
author_facet	Basar, Nurul Syafira
author_sort	Basar, Nurul Syafira
title	Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar
title_short	Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar
title_full	Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar
title_fullStr	Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar
title_full_unstemmed	Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar
title_sort	response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / nurul syafira basar
publisher	Faculty of Applied Sciences
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/25133/ http://ir.uitm.edu.my/id/eprint/25133/1/PPb_NURUL%20SYAFIRA%20BASAR%20AS%20N%2015_5.pdf
first_indexed	2023-09-18T23:14:07Z
last_indexed	2023-09-18T23:14:07Z
_version_	1777418997716746240

Response of leaf explant of curculigo latifolia (hypoxidaceae) towards different hormones treatments in plant tissue culture / Nurul Syafira Basar

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