Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman

The objectives of this study are to extract P. sarmentosum stems using soxhlet extraction technique, to perform the chemical and biological activities of P. sarmentosum stems, to determine the TLC profiling of the extracts sample by using thin layer chromatography method with variety of solvent syst...

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Main Author:	Sahariman, Norhidayah
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2017
Subjects:	QD Chemistry Extraction (Chemistry)
Online Access:	http://ir.uitm.edu.my/id/eprint/26521/ http://ir.uitm.edu.my/id/eprint/26521/1/PPb_NORHIDAYAH%20SAHARIMAN%20AS%20N%2017_5.pdf

id	uitm-26521
recordtype	eprints
spelling	uitm-265212019-11-27T04:34:34Z http://ir.uitm.edu.my/id/eprint/26521/ Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman Sahariman, Norhidayah QD Chemistry Extraction (Chemistry) The objectives of this study are to extract P. sarmentosum stems using soxhlet extraction technique, to perform the chemical and biological activities of P. sarmentosum stems, to determine the TLC profiling of the extracts sample by using thin layer chromatography method with variety of solvent system and to screen the antibacterial activity of plant crude extract using disc diffusion method. Ground P. sarmentosum stems were extracted using soxhlet extraction and using two different type of solvent which are chloroform and ethanol. Then each extract was evaporated using rotary evaporator to produce crude. The phytochemical screening has been performed. During phytochemical screening, phenol and alkaloid has been detected in chloroform extract meanwhile in terpenoid, saponin, flavonoid and tannins have been detected in ethanol extract. TLC profiling of the extracted sample was done using thin layer chromatography method with variety of solvent system. Antibacterial activity was tested between the P. sarmentosum extract against two types of Gram-positive(+) bacteria which were S. aureus andB. subtilis and another two type of Gram-negative (-) bacteria which were S. typhymirium and E. coli by using disc diffusion method. In antibacterial activity, ethanol extract showed the highest inhibition zone against E. coli with 11.50 mm meanwhile, chloroform extract showed the highest inhibition zone against E. coli with 9.00 mm as compared to other bacteria. Faculty of Applied Sciences 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/26521/1/PPb_NORHIDAYAH%20SAHARIMAN%20AS%20N%2017_5.pdf Sahariman, Norhidayah (2017) Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry Extraction (Chemistry)
spellingShingle	QD Chemistry Extraction (Chemistry) Sahariman, Norhidayah Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman
description	The objectives of this study are to extract P. sarmentosum stems using soxhlet extraction technique, to perform the chemical and biological activities of P. sarmentosum stems, to determine the TLC profiling of the extracts sample by using thin layer chromatography method with variety of solvent system and to screen the antibacterial activity of plant crude extract using disc diffusion method. Ground P. sarmentosum stems were extracted using soxhlet extraction and using two different type of solvent which are chloroform and ethanol. Then each extract was evaporated using rotary evaporator to produce crude. The phytochemical screening has been performed. During phytochemical screening, phenol and alkaloid has been detected in chloroform extract meanwhile in terpenoid, saponin, flavonoid and tannins have been detected in ethanol extract. TLC profiling of the extracted sample was done using thin layer chromatography method with variety of solvent system. Antibacterial activity was tested between the P. sarmentosum extract against two types of Gram-positive(+) bacteria which were S. aureus andB. subtilis and another two type of Gram-negative (-) bacteria which were S. typhymirium and E. coli by using disc diffusion method. In antibacterial activity, ethanol extract showed the highest inhibition zone against E. coli with 11.50 mm meanwhile, chloroform extract showed the highest inhibition zone against E. coli with 9.00 mm as compared to other bacteria.
format	Student Project
author	Sahariman, Norhidayah
author_facet	Sahariman, Norhidayah
author_sort	Sahariman, Norhidayah
title	Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman
title_short	Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman
title_full	Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman
title_fullStr	Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman
title_full_unstemmed	Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman
title_sort	phytochemical screening and antibacterial activity of piper sarmentosum stems / norhidayah sahariman
publisher	Faculty of Applied Sciences
publishDate	2017
url	http://ir.uitm.edu.my/id/eprint/26521/ http://ir.uitm.edu.my/id/eprint/26521/1/PPb_NORHIDAYAH%20SAHARIMAN%20AS%20N%2017_5.pdf
first_indexed	2023-09-18T23:16:58Z
last_indexed	2023-09-18T23:16:58Z
_version_	1777419176969764864

Phytochemical screening and antibacterial activity of piper sarmentosum stems / Norhidayah Sahariman

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