Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir

Triglycerides are product formed from the process of transesterification of fatty acid and glycerol. There are many types of triglycerides existed and one of them is tributyrin. Tributyrin is a compound that made up of combination butyric acid an also glycerol. It is a type of triglycerides that na...

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Main Author:	Abd Kadir, Nurul Wahidah
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2017
Subjects:	QD Chemistry
Online Access:	http://ir.uitm.edu.my/id/eprint/26584/ http://ir.uitm.edu.my/id/eprint/26584/1/PPb_NURUL%20WAHIDAH%20ABD%20KADIR%20AS%20N%2017_5.pdf

id	uitm-26584
recordtype	eprints
spelling	uitm-265842019-11-27T03:11:30Z http://ir.uitm.edu.my/id/eprint/26584/ Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir Abd Kadir, Nurul Wahidah QD Chemistry Triglycerides are product formed from the process of transesterification of fatty acid and glycerol. There are many types of triglycerides existed and one of them is tributyrin. Tributyrin is a compound that made up of combination butyric acid an also glycerol. It is a type of triglycerides that naturally present in butter and usually use as an ingredient in making margarine. In this analysis, it is concern to determine the concentration of tributyrin in the sample of margarine. The process started with the step of column chromatography and also thin layer chromatography in order to identify the most suitable solvent mixture and its ratio for the analysis. The best solvent is solvent mixture of hexane with acetonitrile with ratio 70:30.The information about the solvent mixture and the ratio later being further use for the analysis by using high performance liquid chromatography (HPLC). In the step of analysis using HPLC, the effect of flow rates are analyzed on both AR grade and HPLC grade of standard tributyrin to compare the content of impurities. After the best flow rate selected which s 1.0 mLmin- , the analysis are proceeds to the sample injection step to HPLC. Lastly, all results obtained then been used for calculation of concentration of tributyrin in the sample. Faculty of Applied Sciences 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/26584/1/PPb_NURUL%20WAHIDAH%20ABD%20KADIR%20AS%20N%2017_5.pdf Abd Kadir, Nurul Wahidah (2017) Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Abd Kadir, Nurul Wahidah Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir
description	Triglycerides are product formed from the process of transesterification of fatty acid and glycerol. There are many types of triglycerides existed and one of them is tributyrin. Tributyrin is a compound that made up of combination butyric acid an also glycerol. It is a type of triglycerides that naturally present in butter and usually use as an ingredient in making margarine. In this analysis, it is concern to determine the concentration of tributyrin in the sample of margarine. The process started with the step of column chromatography and also thin layer chromatography in order to identify the most suitable solvent mixture and its ratio for the analysis. The best solvent is solvent mixture of hexane with acetonitrile with ratio 70:30.The information about the solvent mixture and the ratio later being further use for the analysis by using high performance liquid chromatography (HPLC). In the step of analysis using HPLC, the effect of flow rates are analyzed on both AR grade and HPLC grade of standard tributyrin to compare the content of impurities. After the best flow rate selected which s 1.0 mLmin- , the analysis are proceeds to the sample injection step to HPLC. Lastly, all results obtained then been used for calculation of concentration of tributyrin in the sample.
format	Student Project
author	Abd Kadir, Nurul Wahidah
author_facet	Abd Kadir, Nurul Wahidah
author_sort	Abd Kadir, Nurul Wahidah
title	Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir
title_short	Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir
title_full	Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir
title_fullStr	Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir
title_full_unstemmed	Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir
title_sort	determination of tributyrin in margarine by using column chromatography (cc), thin layer chromatography (tlc) and high performance liquid chromatography (hplc) / nurul wahidah abd kadir
publisher	Faculty of Applied Sciences
publishDate	2017
url	http://ir.uitm.edu.my/id/eprint/26584/ http://ir.uitm.edu.my/id/eprint/26584/1/PPb_NURUL%20WAHIDAH%20ABD%20KADIR%20AS%20N%2017_5.pdf
first_indexed	2023-09-18T23:17:05Z
last_indexed	2023-09-18T23:17:05Z
_version_	1777419184238493696

Determination of tributyrin in margarine by using column chromatography (CC), thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) / Nurul Wahidah Abd Kadir

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