Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan

SACOL2666, which is known as an N-acetylmuramoyl-L-alanine in NCBI database is shown to be homologuos with atl of S. aureus, a bifunctional autolysin gene. In this study, in-silico analysis of deduced amino acids sequence SACOL2666 was characterized to confirm the protein from S. aureus SHI000 is an...

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Main Author:	Kusnan, Khalida Ain
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Biology
Online Access:	http://ir.uitm.edu.my/id/eprint/26742/ http://ir.uitm.edu.my/id/eprint/26742/1/TM_KHALIDA%20AIN%20KUSNAN%20AS%2014_5.pdf

id	uitm-26742
recordtype	eprints
spelling	uitm-267422019-12-04T02:38:02Z http://ir.uitm.edu.my/id/eprint/26742/ Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan Kusnan, Khalida Ain Biology SACOL2666, which is known as an N-acetylmuramoyl-L-alanine in NCBI database is shown to be homologuos with atl of S. aureus, a bifunctional autolysin gene. In this study, in-silico analysis of deduced amino acids sequence SACOL2666 was characterized to confirm the protein from S. aureus SHI000 is an Nacetylglucosaminidase autolysin. Successful transformed gene in pBAD-sScaB and pQE60-xScaQ clones contain 1860 bp of the full gene and 1779 bp of gene without signal peptide sequence. An N-acetylglucosaminidase protein family (PF01832) of Lysozyme like superfamily is found in SACOL2666 domain architecture. The amino acid of SACOL2666 gene demonstrated a high sequence similarity to characterized N-acetylglucosaminidases, AcmB (L. lactis) and Auto (L. monocytogenes) Group B in GH73 rather than bifunctional autolysins in Group A, Atl (S. aureus). SACOL2666 has high relatedness in sequence similarity (46%) and structural alignment with N-acetylglucosaminidases Auto Chain A structure (3FI7 A). Residue E352, G356, E386, F399, Y455 and a tetrad YATD (Y449-D452) at SACOL2666 hypothetical secondary structures are shown to be identical to Auto (3FI7_A) residues. As conclusion, this study reveals SACOL2666 as a novel N-acetylglucosaminidase with high sequence similarity to N-acetylglucosaminidases in Group B of GH73. Moreover, structural similarity suggests the functional and enzymatic activity of SACOL2666 is similar to Auto (3FI7 A). 2014 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/26742/1/TM_KHALIDA%20AIN%20KUSNAN%20AS%2014_5.pdf Kusnan, Khalida Ain (2014) Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Biology
spellingShingle	Biology Kusnan, Khalida Ain Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan
description	SACOL2666, which is known as an N-acetylmuramoyl-L-alanine in NCBI database is shown to be homologuos with atl of S. aureus, a bifunctional autolysin gene. In this study, in-silico analysis of deduced amino acids sequence SACOL2666 was characterized to confirm the protein from S. aureus SHI000 is an Nacetylglucosaminidase autolysin. Successful transformed gene in pBAD-sScaB and pQE60-xScaQ clones contain 1860 bp of the full gene and 1779 bp of gene without signal peptide sequence. An N-acetylglucosaminidase protein family (PF01832) of Lysozyme like superfamily is found in SACOL2666 domain architecture. The amino acid of SACOL2666 gene demonstrated a high sequence similarity to characterized N-acetylglucosaminidases, AcmB (L. lactis) and Auto (L. monocytogenes) Group B in GH73 rather than bifunctional autolysins in Group A, Atl (S. aureus). SACOL2666 has high relatedness in sequence similarity (46%) and structural alignment with N-acetylglucosaminidases Auto Chain A structure (3FI7 A). Residue E352, G356, E386, F399, Y455 and a tetrad YATD (Y449-D452) at SACOL2666 hypothetical secondary structures are shown to be identical to Auto (3FI7_A) residues. As conclusion, this study reveals SACOL2666 as a novel N-acetylglucosaminidase with high sequence similarity to N-acetylglucosaminidases in Group B of GH73. Moreover, structural similarity suggests the functional and enzymatic activity of SACOL2666 is similar to Auto (3FI7 A).
format	Thesis
author	Kusnan, Khalida Ain
author_facet	Kusnan, Khalida Ain
author_sort	Kusnan, Khalida Ain
title	Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan
title_short	Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan
title_full	Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan
title_fullStr	Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan
title_full_unstemmed	Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan
title_sort	characterization of n-acetylglucosaminidase gene from aureus / khalida ain kusnan
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/26742/ http://ir.uitm.edu.my/id/eprint/26742/1/TM_KHALIDA%20AIN%20KUSNAN%20AS%2014_5.pdf
first_indexed	2023-09-18T23:17:23Z
last_indexed	2023-09-18T23:17:23Z
_version_	1777419203400171520

Characterization of N-acetylglucosaminidase gene from aureus / Khalida Ain Kusnan

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