Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]

TOCOTRIENOL-TOCOPHEROL MIXED FRACTION SUPPLEMENTATION PREVENTS LESION PROGRESSION IN EARLY AND ESTABLISHED ATHEROSCLEROSIS Aishah NM, Omar E, Azlina AR, Nawawi H Faculty of Medicine, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia. Background: Inflammation plays a key role in plaque stabili...

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Main Authors:	Mohd Nawawi, Hapizah, Omar, Effat, Abdul Rahman, Thuhairah Hasrah, Ahmad Saman, Mohd Shahril
Format:	Research Reports
Language:	English
Published:	Research Management Institute (RMI) 2011
Subjects:	Inorganic chemistry Analytical chemistry
Online Access:	http://ir.uitm.edu.my/id/eprint/27112/ http://ir.uitm.edu.my/id/eprint/27112/1/LP_HAPIZAH%20MOHD%20NAWAWI%20RMI%2011_5.pdf

id	uitm-27112
recordtype	eprints
spelling	uitm-271122020-01-09T06:31:04Z http://ir.uitm.edu.my/id/eprint/27112/ Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.] Mohd Nawawi, Hapizah Omar, Effat Abdul Rahman, Thuhairah Hasrah Ahmad Saman, Mohd Shahril Inorganic chemistry Analytical chemistry TOCOTRIENOL-TOCOPHEROL MIXED FRACTION SUPPLEMENTATION PREVENTS LESION PROGRESSION IN EARLY AND ESTABLISHED ATHEROSCLEROSIS Aishah NM, Omar E, Azlina AR, Nawawi H Faculty of Medicine, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia. Background: Inflammation plays a key role in plaque stability and the pathogenesis of atherosclerosis. Palm-derived tocotrienol-tocopherol mixedfraction (TTMF) is a potent antioxidant with anti-inflammatory effects. The effect of TTMF on the development of early and established atherosclerosis remains unclear. Objective: To determine the anti-inflammatory effects of TTMF in rabbits with early and established atherosclerotic lesions. Materials and methods: 60 New Zealand white rabbits were randomized into three major groups which further divided into two groups each with supplementation: (i) TTMF (15mg/kg) or (ii) placebo. Group A were given intervention of TTMF for 2 month followed by high cholesterol diet for either 2 weeks or 2 month with TTMF while group B were induced with high cholesterol diet for either 2 weeks or 2 month before stopped the high cholesterol diet and intervened with TTMF. Group C were given both TTMF and high cholesterol diet concurrently. Blood samples at the intervention interval were analyzed for serum lipid and soluble CRP. At the end of the study, the aortas were evaluated for atherosclerotic lesion using Sudan IV stain and immunohistochemistry staining. Quantitative analysis of the lesion staining was performed using image analysis software. Results: There were significantly reduced atherosclerotic lesions in early (Mean ± SEM; TTMF vs. placebo:7.92 ±2.9 vs. 16.6 ± 1.6 %; p<0.05) and established atherosclerosis (Mean ± SEM; TTMF vs. placebo : 8.45 ± 2.03 vs. 29.47 ± 4.83%; p<0.05) in Group A. Expressions of E-selectin were also significantly reduced in established atherosclerosis in Group A (Mean ± SEM; TTMF vs. placebo: 6.70 ± 1.43 vs. 21.18 ± 2.81 %;p<0.05) and in early atherosclerosis (Mean ± SEM; TTMF vs. placebo : 0.87 ± 0.23 vs. 5.82 ± 2.05 % ; p<0.05) of Bl group. Group C showed no differences in both early and established atherosclerosis with TTMF. However, TTMF gives neutral effects on blood biomarkers of all three groups. Conclusion: TTMF effectively prevents atherosclerotic lesions and inflammatory marker in early and established atherosclerosis. Further studies should emphasis on the tocotrienol isomers for the therapeutic potential of atherosclerosis. Research Management Institute (RMI) 2011 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27112/1/LP_HAPIZAH%20MOHD%20NAWAWI%20RMI%2011_5.pdf Mohd Nawawi, Hapizah and Omar, Effat and Abdul Rahman, Thuhairah Hasrah and Ahmad Saman, Mohd Shahril (2011) Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]. [Research Reports] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Inorganic chemistry Analytical chemistry
spellingShingle	Inorganic chemistry Analytical chemistry Mohd Nawawi, Hapizah Omar, Effat Abdul Rahman, Thuhairah Hasrah Ahmad Saman, Mohd Shahril Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]
description	TOCOTRIENOL-TOCOPHEROL MIXED FRACTION SUPPLEMENTATION PREVENTS LESION PROGRESSION IN EARLY AND ESTABLISHED ATHEROSCLEROSIS Aishah NM, Omar E, Azlina AR, Nawawi H Faculty of Medicine, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia. Background: Inflammation plays a key role in plaque stability and the pathogenesis of atherosclerosis. Palm-derived tocotrienol-tocopherol mixedfraction (TTMF) is a potent antioxidant with anti-inflammatory effects. The effect of TTMF on the development of early and established atherosclerosis remains unclear. Objective: To determine the anti-inflammatory effects of TTMF in rabbits with early and established atherosclerotic lesions. Materials and methods: 60 New Zealand white rabbits were randomized into three major groups which further divided into two groups each with supplementation: (i) TTMF (15mg/kg) or (ii) placebo. Group A were given intervention of TTMF for 2 month followed by high cholesterol diet for either 2 weeks or 2 month with TTMF while group B were induced with high cholesterol diet for either 2 weeks or 2 month before stopped the high cholesterol diet and intervened with TTMF. Group C were given both TTMF and high cholesterol diet concurrently. Blood samples at the intervention interval were analyzed for serum lipid and soluble CRP. At the end of the study, the aortas were evaluated for atherosclerotic lesion using Sudan IV stain and immunohistochemistry staining. Quantitative analysis of the lesion staining was performed using image analysis software. Results: There were significantly reduced atherosclerotic lesions in early (Mean ± SEM; TTMF vs. placebo:7.92 ±2.9 vs. 16.6 ± 1.6 %; p<0.05) and established atherosclerosis (Mean ± SEM; TTMF vs. placebo : 8.45 ± 2.03 vs. 29.47 ± 4.83%; p<0.05) in Group A. Expressions of E-selectin were also significantly reduced in established atherosclerosis in Group A (Mean ± SEM; TTMF vs. placebo: 6.70 ± 1.43 vs. 21.18 ± 2.81 %;p<0.05) and in early atherosclerosis (Mean ± SEM; TTMF vs. placebo : 0.87 ± 0.23 vs. 5.82 ± 2.05 % ; p<0.05) of Bl group. Group C showed no differences in both early and established atherosclerosis with TTMF. However, TTMF gives neutral effects on blood biomarkers of all three groups. Conclusion: TTMF effectively prevents atherosclerotic lesions and inflammatory marker in early and established atherosclerosis. Further studies should emphasis on the tocotrienol isomers for the therapeutic potential of atherosclerosis.
format	Research Reports
author	Mohd Nawawi, Hapizah Omar, Effat Abdul Rahman, Thuhairah Hasrah Ahmad Saman, Mohd Shahril
author_facet	Mohd Nawawi, Hapizah Omar, Effat Abdul Rahman, Thuhairah Hasrah Ahmad Saman, Mohd Shahril
author_sort	Mohd Nawawi, Hapizah
title	Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]
title_short	Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]
title_full	Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]
title_fullStr	Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]
title_full_unstemmed	Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]
title_sort	effect of tocotrienol on plaque stability in early and established atherosclerosis / hapizah mohd nawawi … [et_al.]
publisher	Research Management Institute (RMI)
publishDate	2011
url	http://ir.uitm.edu.my/id/eprint/27112/ http://ir.uitm.edu.my/id/eprint/27112/1/LP_HAPIZAH%20MOHD%20NAWAWI%20RMI%2011_5.pdf
first_indexed	2023-09-18T23:17:48Z
last_indexed	2023-09-18T23:17:48Z
_version_	1777419229060923392

Effect of tocotrienol on plaque stability in early and established atherosclerosis / Hapizah Mohd Nawawi … [et_al.]

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