Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa

Introduction: Thermophilic bacteria can be isolated at high temperatures (60°C to 80°C) in hot springs of neutral or alkaline pH, as well as in hot water or in natural waters subjected to thermal population. This is in contrast to mesophiles which normally survive at 37°C. Objective: To isolate and...

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Main Authors:	Hassan, Zuridah, Allauddin, Zeenathul Nazariah, Issa, Rahizan
Format:	Research Reports
Language:	English
Published:	Research Management Institute (RMI) 2010
Subjects:	Bacteria
Online Access:	http://ir.uitm.edu.my/id/eprint/27169/ http://ir.uitm.edu.my/id/eprint/27169/1/LP_ZURIDAH%20HASSAN%20RMI%2010_5.pdf

id	uitm-27169
recordtype	eprints
spelling	uitm-271692020-01-10T05:18:51Z http://ir.uitm.edu.my/id/eprint/27169/ Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa Hassan, Zuridah Allauddin, Zeenathul Nazariah Issa, Rahizan Bacteria Introduction: Thermophilic bacteria can be isolated at high temperatures (60°C to 80°C) in hot springs of neutral or alkaline pH, as well as in hot water or in natural waters subjected to thermal population. This is in contrast to mesophiles which normally survive at 37°C. Objective: To isolate and characterize thermophilic bacteria from the hot springs in Malaysia and to identify useful products from these bacteria. Materials and Methods: Water samples from six hot springs (Selayang, Setapak, Klah, Labis, Gadek, and Pedas) were collected using the LaMotte bottom sampling dredge and transported in cold boxes back to the laboratory at UiTM. Portions of these water samples were mixed with CTYE medium and another portion without CTYE medium and were incubated at 60°C for 1 to 2 days. After 24 h incubation, turbid samples were plated and incubated at the same temperature using 3% agar in CTYE medium. Pure colonies were chosen from each culture plate for identification by (i) conventional biochemical methods and (ii) molecular characterization. DNA were extracted from positive cultures and amplified for 16S rDNA gene by polymerase chain method. The amplicons were sent for sequencing and results obtained were aligned with representative 16S rDNA sequences of related taxa using CLUSTAL W 1.83 software. Reference strain Thermus aquaticus (ATCC® 25104™) was included as control. Results: Most of the organisms from the hot springs can be maintained in the laboratory using CTYE medium at 60°C. The water temperature ranged from 48°C to 95°C with pH 6.8 to 7.9. By conventional tests and microscopic examination, five new strains of bacteria (Klah, Klah 2, Labis, Gadek, and Pedas) were isolated and confirmed to be closely related to Geobacillus spp. These bacteria were Grampositives, motile, oxidase and catalase positive. In these same locations, another four Gr$m negative bacilli (Sly, Sly 2, Klah 1, and Gadek 1) and three Gram-positive spore-forming bacilli (Sly 1, Klah 3, Gadek 2) were also found. Out of the four Gramnegatives, two strains (Gadek 1 and Klah 1) were found to be closely related to Thermus strains. All the Gram-positives were related to thermophilic Bacillus spp. Based on 16S rDNA study, five new strains of bacteria (Klah, Klah 2, Labis, Gadek, and Pedas) were confirmed to be closely related to Geobacillus spp. (Gram-positive bacilli, motile, oxidase and catalase positive). By skim milk test, five strains (Gadek, Gadek2, Labis, Klah3, and Klah2) were suspected to be protease producers out of eight thermophiles (Gadek, Gadek2, Labis, Pedas, Klah3, Klah2, Sly, and Slyl) that Research Management Institute (RMI) 2010-04 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27169/1/LP_ZURIDAH%20HASSAN%20RMI%2010_5.pdf Hassan, Zuridah and Allauddin, Zeenathul Nazariah and Issa, Rahizan (2010) Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa. [Research Reports] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Bacteria
spellingShingle	Bacteria Hassan, Zuridah Allauddin, Zeenathul Nazariah Issa, Rahizan Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa
description	Introduction: Thermophilic bacteria can be isolated at high temperatures (60°C to 80°C) in hot springs of neutral or alkaline pH, as well as in hot water or in natural waters subjected to thermal population. This is in contrast to mesophiles which normally survive at 37°C. Objective: To isolate and characterize thermophilic bacteria from the hot springs in Malaysia and to identify useful products from these bacteria. Materials and Methods: Water samples from six hot springs (Selayang, Setapak, Klah, Labis, Gadek, and Pedas) were collected using the LaMotte bottom sampling dredge and transported in cold boxes back to the laboratory at UiTM. Portions of these water samples were mixed with CTYE medium and another portion without CTYE medium and were incubated at 60°C for 1 to 2 days. After 24 h incubation, turbid samples were plated and incubated at the same temperature using 3% agar in CTYE medium. Pure colonies were chosen from each culture plate for identification by (i) conventional biochemical methods and (ii) molecular characterization. DNA were extracted from positive cultures and amplified for 16S rDNA gene by polymerase chain method. The amplicons were sent for sequencing and results obtained were aligned with representative 16S rDNA sequences of related taxa using CLUSTAL W 1.83 software. Reference strain Thermus aquaticus (ATCC® 25104™) was included as control. Results: Most of the organisms from the hot springs can be maintained in the laboratory using CTYE medium at 60°C. The water temperature ranged from 48°C to 95°C with pH 6.8 to 7.9. By conventional tests and microscopic examination, five new strains of bacteria (Klah, Klah 2, Labis, Gadek, and Pedas) were isolated and confirmed to be closely related to Geobacillus spp. These bacteria were Grampositives, motile, oxidase and catalase positive. In these same locations, another four Gr$m negative bacilli (Sly, Sly 2, Klah 1, and Gadek 1) and three Gram-positive spore-forming bacilli (Sly 1, Klah 3, Gadek 2) were also found. Out of the four Gramnegatives, two strains (Gadek 1 and Klah 1) were found to be closely related to Thermus strains. All the Gram-positives were related to thermophilic Bacillus spp. Based on 16S rDNA study, five new strains of bacteria (Klah, Klah 2, Labis, Gadek, and Pedas) were confirmed to be closely related to Geobacillus spp. (Gram-positive bacilli, motile, oxidase and catalase positive). By skim milk test, five strains (Gadek, Gadek2, Labis, Klah3, and Klah2) were suspected to be protease producers out of eight thermophiles (Gadek, Gadek2, Labis, Pedas, Klah3, Klah2, Sly, and Slyl) that
format	Research Reports
author	Hassan, Zuridah Allauddin, Zeenathul Nazariah Issa, Rahizan
author_facet	Hassan, Zuridah Allauddin, Zeenathul Nazariah Issa, Rahizan
author_sort	Hassan, Zuridah
title	Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa
title_short	Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa
title_full	Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa
title_fullStr	Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa
title_full_unstemmed	Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa
title_sort	characterization of novel thermophilic microorganisms from hot springs / zuridah hassan, zeenathul nazariah allauddin and rahizan issa
publisher	Research Management Institute (RMI)
publishDate	2010
url	http://ir.uitm.edu.my/id/eprint/27169/ http://ir.uitm.edu.my/id/eprint/27169/1/LP_ZURIDAH%20HASSAN%20RMI%2010_5.pdf
first_indexed	2023-09-18T23:17:55Z
last_indexed	2023-09-18T23:17:55Z
_version_	1777419236599136256

Characterization of novel thermophilic microorganisms from hot springs / Zuridah Hassan, Zeenathul Nazariah Allauddin and Rahizan Issa

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