Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat

Pasteurella multocida (P. multocida) serotype B is associated with hemorrhagic septicaemia (HS) disease endemic in Africa, India and Asian countries. It is causative agent of thriftily significant disease in livestock. Hence, this study purposed and aimed to identify and express an immunogenic solub...

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Main Author:	Jumahat, Noor Masyitah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	RM Therapeutics. Pharmacology Immunotherapy. Serotherapy
Online Access:	http://ir.uitm.edu.my/id/eprint/27214/ http://ir.uitm.edu.my/id/eprint/27214/1/TM_NOOR%20MASYITAH%20JUMAHAT%20MD%2015_5.pdf

id	uitm-27214
recordtype	eprints
spelling	uitm-272142020-01-14T02:07:13Z http://ir.uitm.edu.my/id/eprint/27214/ Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat Jumahat, Noor Masyitah RM Therapeutics. Pharmacology Immunotherapy. Serotherapy Pasteurella multocida (P. multocida) serotype B is associated with hemorrhagic septicaemia (HS) disease endemic in Africa, India and Asian countries. It is causative agent of thriftily significant disease in livestock. Hence, this study purposed and aimed to identify and express an immunogenic soluble protein of P. multocida in efforts toward development of HS vaccine. Immunogenic soluble protein was identified as lipoprotein B (plpB) using electrospray mass spectrometry. The size of expressed purified recombinant protein was approximately 39kDa. Immunogenicity study of the recombinant protein plpB was carried out using 6 groups o f BALB/c mice. The groups were immunized with recombinant protein (Group 1), soluble recombinant protein (Group 2), insoluble recombinant protein (Group 3), vector (Group 4), soluble protein o f P. multocida (Group 5) and PBS (Group 6 ) respectively. Mice in group 4 and 6 showed signs and symptom of HS after challenge with the parental strain (p-value < 0.05). However, immunised mice with purified recombinant protein did not show signs and symptoms of HS. Based on immunoblotting analysis, purified recombinant protein was significantly immunogenic (p-value < 0.05). Additionally, no inflammation was seen in the tissues o f organs from mice immunized with purified recombinant protein, which indicates that recombinant protein was 100% protective towards P. multocida infection and eventually towards HS disease. Thus, this study shows that the recombinant protein lipoprotein B (plpB) is significantly immunogenic and could be a potential candidate in developing vaccine against HS. 2015-04 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27214/1/TM_NOOR%20MASYITAH%20JUMAHAT%20MD%2015_5.pdf Jumahat, Noor Masyitah (2015) Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology Immunotherapy. Serotherapy
spellingShingle	RM Therapeutics. Pharmacology Immunotherapy. Serotherapy Jumahat, Noor Masyitah Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
description	Pasteurella multocida (P. multocida) serotype B is associated with hemorrhagic septicaemia (HS) disease endemic in Africa, India and Asian countries. It is causative agent of thriftily significant disease in livestock. Hence, this study purposed and aimed to identify and express an immunogenic soluble protein of P. multocida in efforts toward development of HS vaccine. Immunogenic soluble protein was identified as lipoprotein B (plpB) using electrospray mass spectrometry. The size of expressed purified recombinant protein was approximately 39kDa. Immunogenicity study of the recombinant protein plpB was carried out using 6 groups o f BALB/c mice. The groups were immunized with recombinant protein (Group 1), soluble recombinant protein (Group 2), insoluble recombinant protein (Group 3), vector (Group 4), soluble protein o f P. multocida (Group 5) and PBS (Group 6 ) respectively. Mice in group 4 and 6 showed signs and symptom of HS after challenge with the parental strain (p-value < 0.05). However, immunised mice with purified recombinant protein did not show signs and symptoms of HS. Based on immunoblotting analysis, purified recombinant protein was significantly immunogenic (p-value < 0.05). Additionally, no inflammation was seen in the tissues o f organs from mice immunized with purified recombinant protein, which indicates that recombinant protein was 100% protective towards P. multocida infection and eventually towards HS disease. Thus, this study shows that the recombinant protein lipoprotein B (plpB) is significantly immunogenic and could be a potential candidate in developing vaccine against HS.
format	Thesis
author	Jumahat, Noor Masyitah
author_facet	Jumahat, Noor Masyitah
author_sort	Jumahat, Noor Masyitah
title	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_short	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_full	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_fullStr	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_full_unstemmed	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_sort	identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype b / noor masyitah jumahat
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/27214/ http://ir.uitm.edu.my/id/eprint/27214/1/TM_NOOR%20MASYITAH%20JUMAHAT%20MD%2015_5.pdf
first_indexed	2023-09-18T23:18:01Z
last_indexed	2023-09-18T23:18:01Z
_version_	1777419242999644160

Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat

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