Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani

The biodegradability of petroleum waste product was evaluated using oil sludge taken from Exxon Mobil petroleum refiner y treatment plant in Kerteh, Terengganu. This study was a further extension of studies conducted by Dzulkafli (2007) where the qualitative analysis was established. The analysis in...

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Main Author:	Abdul Ghani, Norhafiza
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Biodegradation TD Environmental technology. Sanitary engineering
Online Access:	http://ir.uitm.edu.my/id/eprint/27241/ http://ir.uitm.edu.my/id/eprint/27241/1/TM_NORHAFIZA%20ABDUL%20GHANI%20EC%2008_5.pdf

id	uitm-27241
recordtype	eprints
spelling	uitm-272412020-01-14T01:46:26Z http://ir.uitm.edu.my/id/eprint/27241/ Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani Abdul Ghani, Norhafiza Biodegradation TD Environmental technology. Sanitary engineering The biodegradability of petroleum waste product was evaluated using oil sludge taken from Exxon Mobil petroleum refiner y treatment plant in Kerteh, Terengganu. This study was a further extension of studies conducted by Dzulkafli (2007) where the qualitative analysis was established. The analysis involves the utilization of bacterial strains most effective in PAHs degradation as classified by Dzulkafli (2007). The PAHs adopted were phenanthrene, anthracene and dibenzothiophene which were used as source of carbon. PCR and DNA Sequencing were conducted to confirm the identity of bacterial strain classified by Dzulkafli (2007). These molecular technique s provide s up to 90% accuracy of identification. Using these techniques these three strains identified belong to genus of Pseudomonas namely; Uncultured Pseudomonas sp. clone 2-A, Pseudomonas Stutzeri strain HS-D36 and Pseudomonas Stutzeri strain LS40 1. Turbidity test was conducted for growth rates of each strain. Pseudomonas Stutzeri strain HS-D36 indicated the highest growth rates followed by Uncultured Pseudomonas sp. clone 2-A and Pseudomonas Stutzeri strain LS401. Identification of the most effective PAH degrading by bacterial strain was established by determining of average degradation rates. Anthracene proved to be the most degraded by these strains with the highest average degradation rate followed by phenanthrene and dibenzothiophene. While, Pseudomonas Stutzeri strain HS-D36 revealed to be the best strain in degrading PAHs, with the highest degradation followed by Uncultured Pseudomonas sp. clone 2-A and Pseudomonas Stutzeri strain LS401. 2008 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27241/1/TM_NORHAFIZA%20ABDUL%20GHANI%20EC%2008_5.pdf Abdul Ghani, Norhafiza (2008) Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Biodegradation TD Environmental technology. Sanitary engineering
spellingShingle	Biodegradation TD Environmental technology. Sanitary engineering Abdul Ghani, Norhafiza Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
description	The biodegradability of petroleum waste product was evaluated using oil sludge taken from Exxon Mobil petroleum refiner y treatment plant in Kerteh, Terengganu. This study was a further extension of studies conducted by Dzulkafli (2007) where the qualitative analysis was established. The analysis involves the utilization of bacterial strains most effective in PAHs degradation as classified by Dzulkafli (2007). The PAHs adopted were phenanthrene, anthracene and dibenzothiophene which were used as source of carbon. PCR and DNA Sequencing were conducted to confirm the identity of bacterial strain classified by Dzulkafli (2007). These molecular technique s provide s up to 90% accuracy of identification. Using these techniques these three strains identified belong to genus of Pseudomonas namely; Uncultured Pseudomonas sp. clone 2-A, Pseudomonas Stutzeri strain HS-D36 and Pseudomonas Stutzeri strain LS40 1. Turbidity test was conducted for growth rates of each strain. Pseudomonas Stutzeri strain HS-D36 indicated the highest growth rates followed by Uncultured Pseudomonas sp. clone 2-A and Pseudomonas Stutzeri strain LS401. Identification of the most effective PAH degrading by bacterial strain was established by determining of average degradation rates. Anthracene proved to be the most degraded by these strains with the highest average degradation rate followed by phenanthrene and dibenzothiophene. While, Pseudomonas Stutzeri strain HS-D36 revealed to be the best strain in degrading PAHs, with the highest degradation followed by Uncultured Pseudomonas sp. clone 2-A and Pseudomonas Stutzeri strain LS401.
format	Thesis
author	Abdul Ghani, Norhafiza
author_facet	Abdul Ghani, Norhafiza
author_sort	Abdul Ghani, Norhafiza
title	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_short	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_full	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_fullStr	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_full_unstemmed	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_sort	quantitative determination on biodegradation of pahs by bacteria from oil sludge / norhafiza abdul ghani
publishDate	2008
url	http://ir.uitm.edu.my/id/eprint/27241/ http://ir.uitm.edu.my/id/eprint/27241/1/TM_NORHAFIZA%20ABDUL%20GHANI%20EC%2008_5.pdf
first_indexed	2023-09-18T23:18:05Z
last_indexed	2023-09-18T23:18:05Z
_version_	1777419246775566336

Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani

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