The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman

Breast cancer is a major problem among females all over the world. It is the most common cancer among Malaysian women with a prevalence of 39.5 per 100 000 population and consisting of 31% of all female cancers in 2003. There were many factors contributed to this disease including social demography,...

Full description

Bibliographic Details
Main Author: Osman, Nur Azimah
Format: Thesis
Language:English
Published: 2009
Subjects:
Online Access:http://ir.uitm.edu.my/id/eprint/27259/
http://ir.uitm.edu.my/id/eprint/27259/1/TM_NUR%20AZIMAH%20OSMAN%20AS%2009_5.pdf
id uitm-27259
recordtype eprints
spelling uitm-272592020-01-15T02:31:23Z http://ir.uitm.edu.my/id/eprint/27259/ The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman Osman, Nur Azimah Cancer Research. Experimentation Breast cancer is a major problem among females all over the world. It is the most common cancer among Malaysian women with a prevalence of 39.5 per 100 000 population and consisting of 31% of all female cancers in 2003. There were many factors contributed to this disease including social demography, environmental and genetic. Tumor marker is a substance produces by tumor cells in order to distinguish tumor and normal cells. A total of 26 section paraffinized breast cancer tissues were used to study the relationship between cancer markers and social demography. All the paraffinized breast cancer tissues were reclassified according to the histologic grade (Nottingham Grading System) into grade I (9 samples), grade II (9 samples), grade III (6 samples) and there were 2 samples with unknown grade. Statistical analysis using chi square analysis shows that there was significant difference between Nottingham grading system with hematoxylin and eosin staining (< 0.05). H&E staining is not suitable to be applied as a method for breast cancer grading. Four breast cancer markers namely estrogen receptor (ER), progesterone receptor (PR), c-erbB-2 and hMAM were related with the age of breast cancer patients. The mean age of the patient s was 53.3 (SD=10, median 52). Genomic DNA extractions from the samples were achieved by using DNeasy Blood and Tissue Kit (Qiagen, Germany). The detection of hMAM DNA amplification was investigated by using Polymerase Chain Reaction (PCR). Breast cancer cell line (MCF-7), serum and plasma were used as positive controls whilst serum and plasma from normal patients were used as negative controls for the amplification of hMAM DNA. The relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue were studied by categorical and relationship modeling (C&R). The variation of relationship between them could provide a better prognostic for the management of breast cancer patients. 2009 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27259/1/TM_NUR%20AZIMAH%20OSMAN%20AS%2009_5.pdf Osman, Nur Azimah (2009) The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman. Masters thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Cancer
Research. Experimentation
spellingShingle Cancer
Research. Experimentation
Osman, Nur Azimah
The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman
description Breast cancer is a major problem among females all over the world. It is the most common cancer among Malaysian women with a prevalence of 39.5 per 100 000 population and consisting of 31% of all female cancers in 2003. There were many factors contributed to this disease including social demography, environmental and genetic. Tumor marker is a substance produces by tumor cells in order to distinguish tumor and normal cells. A total of 26 section paraffinized breast cancer tissues were used to study the relationship between cancer markers and social demography. All the paraffinized breast cancer tissues were reclassified according to the histologic grade (Nottingham Grading System) into grade I (9 samples), grade II (9 samples), grade III (6 samples) and there were 2 samples with unknown grade. Statistical analysis using chi square analysis shows that there was significant difference between Nottingham grading system with hematoxylin and eosin staining (< 0.05). H&E staining is not suitable to be applied as a method for breast cancer grading. Four breast cancer markers namely estrogen receptor (ER), progesterone receptor (PR), c-erbB-2 and hMAM were related with the age of breast cancer patients. The mean age of the patient s was 53.3 (SD=10, median 52). Genomic DNA extractions from the samples were achieved by using DNeasy Blood and Tissue Kit (Qiagen, Germany). The detection of hMAM DNA amplification was investigated by using Polymerase Chain Reaction (PCR). Breast cancer cell line (MCF-7), serum and plasma were used as positive controls whilst serum and plasma from normal patients were used as negative controls for the amplification of hMAM DNA. The relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue were studied by categorical and relationship modeling (C&R). The variation of relationship between them could provide a better prognostic for the management of breast cancer patients.
format Thesis
author Osman, Nur Azimah
author_facet Osman, Nur Azimah
author_sort Osman, Nur Azimah
title The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman
title_short The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman
title_full The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman
title_fullStr The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman
title_full_unstemmed The study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / Nur Azimah Osman
title_sort study of relationship between cancer markers and age of patient in primary and metastatic paraffinized breast cancer tissue / nur azimah osman
publishDate 2009
url http://ir.uitm.edu.my/id/eprint/27259/
http://ir.uitm.edu.my/id/eprint/27259/1/TM_NUR%20AZIMAH%20OSMAN%20AS%2009_5.pdf
first_indexed 2023-09-18T23:18:07Z
last_indexed 2023-09-18T23:18:07Z
_version_ 1777419249373937664