Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim

Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim

The aim of the study was to determine the anti-ulcer effect of Elaeis guineensis Jacq. (Oil palm) leaves ethanolic extract on ethanol-induced ulcer in rats. In the present study, the rats were pre-treated with E. guineensis leaves (100, 200 and 500 mg/kg b.wt.). The phytochemical contents of the lea...

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Main Author: Abd Rahim, Nurusyifa’
Format: Thesis
Language:English
Published: 2016
Subjects:
Antioxidants
Online Access:http://ir.uitm.edu.my/id/eprint/27324/
http://ir.uitm.edu.my/id/eprint/27324/1/TM_NURUSYIFA%27%20ABD%20RAHIM%20AS%2016_5.pdf
id uitm-27324
recordtype eprints
spelling uitm-273242020-01-16T03:30:41Z http://ir.uitm.edu.my/id/eprint/27324/ Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim Abd Rahim, Nurusyifa’ Antioxidants The aim of the study was to determine the anti-ulcer effect of Elaeis guineensis Jacq. (Oil palm) leaves ethanolic extract on ethanol-induced ulcer in rats. In the present study, the rats were pre-treated with E. guineensis leaves (100, 200 and 500 mg/kg b.wt.). The phytochemical contents of the leaves were screened using Gas Chromatography Mass Spectrophotometer (GC-MS). Meanwhile, antioxidant activity was evaluated using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay with five different concentrations (0.01, 0.05, 0.1, 0.15 and 0.2 mg/mL). From the results, the rats pre-treated with 100 mg/kg b.wt. Significantly (p<0.05) showed gastroprotective effect where only a small area of hemorrhagic lesions appeared on the gastric mucosal layer. Based on the phytochemical screening by GC-MS, the presence of phytol was detected. Phytol is known as diterpenoids, which can be found in chlorophyll and tocopherol. They are known as strong immunomodulatory agent, which is known as one of the components that can increase human immune system in fighting antigens. Antioxidant assay exhibited good antioxidant activity from the ethanolic extract with IC50 value of 0.0817 mg/mL. IC50 value less than 10 mg/mL is considered as an effective antioxidant. The extract at high concentration (0.2 mg/mL) showed the highest percentage of DPPH inhibition by antioxidant with 67.06%. Thus, explaining the extract at high concentration has higher antioxidant activity compare to lower concentration. In the anti-ulcer study, an increase in concentration E. guineensis (0.2 mg/mL), led to an increase in ulcer area. This could be due to the fact that phytol are potentially toxic at high concentration, although they are also good immunomodulatory agents. The extract at low concentration showed an effective gastroprotective activity as compared to the extracts at higher concentrations. Thus, E. guineensis leaves appear to have therapeutic potential in preventing gastric ulceration induced by ethanol. 2016 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27324/1/TM_NURUSYIFA%27%20ABD%20RAHIM%20AS%2016_5.pdf Abd Rahim, Nurusyifa’ (2016) Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim. Masters thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Antioxidants
spellingShingle Antioxidants
Abd Rahim, Nurusyifa’
Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
description The aim of the study was to determine the anti-ulcer effect of Elaeis guineensis Jacq. (Oil palm) leaves ethanolic extract on ethanol-induced ulcer in rats. In the present study, the rats were pre-treated with E. guineensis leaves (100, 200 and 500 mg/kg b.wt.). The phytochemical contents of the leaves were screened using Gas Chromatography Mass Spectrophotometer (GC-MS). Meanwhile, antioxidant activity was evaluated using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay with five different concentrations (0.01, 0.05, 0.1, 0.15 and 0.2 mg/mL). From the results, the rats pre-treated with 100 mg/kg b.wt. Significantly (p<0.05) showed gastroprotective effect where only a small area of hemorrhagic lesions appeared on the gastric mucosal layer. Based on the phytochemical screening by GC-MS, the presence of phytol was detected. Phytol is known as diterpenoids, which can be found in chlorophyll and tocopherol. They are known as strong immunomodulatory agent, which is known as one of the components that can increase human immune system in fighting antigens. Antioxidant assay exhibited good antioxidant activity from the ethanolic extract with IC50 value of 0.0817 mg/mL. IC50 value less than 10 mg/mL is considered as an effective antioxidant. The extract at high concentration (0.2 mg/mL) showed the highest percentage of DPPH inhibition by antioxidant with 67.06%. Thus, explaining the extract at high concentration has higher antioxidant activity compare to lower concentration. In the anti-ulcer study, an increase in concentration E. guineensis (0.2 mg/mL), led to an increase in ulcer area. This could be due to the fact that phytol are potentially toxic at high concentration, although they are also good immunomodulatory agents. The extract at low concentration showed an effective gastroprotective activity as compared to the extracts at higher concentrations. Thus, E. guineensis leaves appear to have therapeutic potential in preventing gastric ulceration induced by ethanol.
format Thesis
author Abd Rahim, Nurusyifa’
author_facet Abd Rahim, Nurusyifa’
author_sort Abd Rahim, Nurusyifa’
title Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_short Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_full Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_fullStr Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_full_unstemmed Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_sort antioxidant and anti-ulcer effect of elaeis guineensis jacq. (oil palm) leaves ethanolic extract on experimental rats / nurusyifa’ abd rahim
publishDate 2016
url http://ir.uitm.edu.my/id/eprint/27324/
http://ir.uitm.edu.my/id/eprint/27324/1/TM_NURUSYIFA%27%20ABD%20RAHIM%20AS%2016_5.pdf
first_indexed 2023-09-18T23:18:16Z
last_indexed 2023-09-18T23:18:16Z
_version_ 1777419258559463424

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