Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot

Human cytomegalovirus (HCMV) is a causative agent for HCMV infection that serves as the main cause of morbidity and mortality in immunocompetent individuals such as immunosuppressed recipient in solid organ transplant (SOT), immunod eficiency patients in the case of HIV and AIDS, cancer patients and...

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Main Author:	Febrie Amot, Issabella
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Infectious and parasitic diseases
Online Access:	http://ir.uitm.edu.my/id/eprint/27362/ http://ir.uitm.edu.my/id/eprint/27362/1/TD_ISSABELLA%20FEBRIE%20AMOT%20HS%2015_5.pdf

id	uitm-27362
recordtype	eprints
spelling	uitm-273622020-01-21T03:30:45Z http://ir.uitm.edu.my/id/eprint/27362/ Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot Febrie Amot, Issabella Infectious and parasitic diseases Human cytomegalovirus (HCMV) is a causative agent for HCMV infection that serves as the main cause of morbidity and mortality in immunocompetent individuals such as immunosuppressed recipient in solid organ transplant (SOT), immunod eficiency patients in the case of HIV and AIDS, cancer patients and infants. In Malaysia, there are few studies that are conducted focusing on the detection of HCMV diagnosis marker such as HCMV DNA among population in tertiary hospitals. Thus, in this preliminary study, the objective is to detect the presence of HCMV DNA in HCMV IgM and IgG positive samples. A total of 66 HCMV positive samples that consists of 33 IgM and IgG serum respectively, are collected from the Virology Laboratory under the Department of Pathology in HUKL. These samples are extracted according to the provided protocol by the manufactures, in order to get the purified DNA for the real time polymerase reaction (qPCR) assay. Higher concentration of HCMV DNA is detected in HCMV IgM samples with high specificity of the amplification reaction. Whereas, the HCMV DNA detection is lower in IgG samples and the specificity of the amplification reaction is also less specific. The validity and specificity of qPCR assay for both sample groups are achieved. In conclusion, HCMV DNA is detectable in serum samples using qPCR detection assay with a higher percentage (100%) of HCMV DNA concentrations are detected in the HCMV IgM positive samples but a lower percentage (79%) is seen HCMV IgG positive samples. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27362/1/TD_ISSABELLA%20FEBRIE%20AMOT%20HS%2015_5.pdf Febrie Amot, Issabella (2015) Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot. Degree thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Infectious and parasitic diseases
spellingShingle	Infectious and parasitic diseases Febrie Amot, Issabella Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot
description	Human cytomegalovirus (HCMV) is a causative agent for HCMV infection that serves as the main cause of morbidity and mortality in immunocompetent individuals such as immunosuppressed recipient in solid organ transplant (SOT), immunod eficiency patients in the case of HIV and AIDS, cancer patients and infants. In Malaysia, there are few studies that are conducted focusing on the detection of HCMV diagnosis marker such as HCMV DNA among population in tertiary hospitals. Thus, in this preliminary study, the objective is to detect the presence of HCMV DNA in HCMV IgM and IgG positive samples. A total of 66 HCMV positive samples that consists of 33 IgM and IgG serum respectively, are collected from the Virology Laboratory under the Department of Pathology in HUKL. These samples are extracted according to the provided protocol by the manufactures, in order to get the purified DNA for the real time polymerase reaction (qPCR) assay. Higher concentration of HCMV DNA is detected in HCMV IgM samples with high specificity of the amplification reaction. Whereas, the HCMV DNA detection is lower in IgG samples and the specificity of the amplification reaction is also less specific. The validity and specificity of qPCR assay for both sample groups are achieved. In conclusion, HCMV DNA is detectable in serum samples using qPCR detection assay with a higher percentage (100%) of HCMV DNA concentrations are detected in the HCMV IgM positive samples but a lower percentage (79%) is seen HCMV IgG positive samples.
format	Thesis
author	Febrie Amot, Issabella
author_facet	Febrie Amot, Issabella
author_sort	Febrie Amot, Issabella
title	Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot
title_short	Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot
title_full	Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot
title_fullStr	Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot
title_full_unstemmed	Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot
title_sort	detection of human cytomegalovirus (hcmv) dna in hcmv igm and igg positive samples / issabella febrie amot
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/27362/ http://ir.uitm.edu.my/id/eprint/27362/1/TD_ISSABELLA%20FEBRIE%20AMOT%20HS%2015_5.pdf
first_indexed	2023-09-18T23:18:19Z
last_indexed	2023-09-18T23:18:19Z
_version_	1777419262248353792

Detection of human cytomegalovirus (HCMV) DNA in HCMV IgM and IgG positive samples / Issabella Febrie Amot

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