Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin

The weakest weak D called DEL phenotype was able to be detected via serological adsorption elution and molecular genotyping. In this study, the freeze-thaw elution and ssp-peR were used to detect the DEL phenotype. A total of 43 RhD negative samples collected from the National Blood Centre were sero...

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Main Author:	Nordin, Nordiana
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Clinical pathology. Laboratory technique Examination of the blood
Online Access:	http://ir.uitm.edu.my/id/eprint/27365/ http://ir.uitm.edu.my/id/eprint/27365/1/TD_NORDIANA%20NORDIN%20HS%2015_5.pdf

id	uitm-27365
recordtype	eprints
spelling	uitm-273652020-01-21T03:43:48Z http://ir.uitm.edu.my/id/eprint/27365/ Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin Nordin, Nordiana Clinical pathology. Laboratory technique Examination of the blood The weakest weak D called DEL phenotype was able to be detected via serological adsorption elution and molecular genotyping. In this study, the freeze-thaw elution and ssp-peR were used to detect the DEL phenotype. A total of 43 RhD negative samples collected from the National Blood Centre were serologically tested for Rh phenotyping and screened for DEL phenotype by both serological freeze-thaw elution and SSP-PCR. Based on the Rh phenotyping, the 43 RhD negative samples consisted of 34 ccee phenotype, 6 Ccee phenotype, 2 ccEe phenotype and 1 CCee phenotype. No DEL phenotype was detected by serological freeze-thaw elution but 2.33% (1/43) from the samples were detected for DEL phenotype that carried the RHD1227A allele by SSP-PCR. As a conclusion, the aim of this study was accomplished by the ability to detect DEL phenotype via molecular genotyping technique in distinguishing the DEL phenotype from truly D negative but not for freeze thaw elution technique. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27365/1/TD_NORDIANA%20NORDIN%20HS%2015_5.pdf Nordin, Nordiana (2015) Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin. Degree thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Clinical pathology. Laboratory technique Examination of the blood
spellingShingle	Clinical pathology. Laboratory technique Examination of the blood Nordin, Nordiana Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin
description	The weakest weak D called DEL phenotype was able to be detected via serological adsorption elution and molecular genotyping. In this study, the freeze-thaw elution and ssp-peR were used to detect the DEL phenotype. A total of 43 RhD negative samples collected from the National Blood Centre were serologically tested for Rh phenotyping and screened for DEL phenotype by both serological freeze-thaw elution and SSP-PCR. Based on the Rh phenotyping, the 43 RhD negative samples consisted of 34 ccee phenotype, 6 Ccee phenotype, 2 ccEe phenotype and 1 CCee phenotype. No DEL phenotype was detected by serological freeze-thaw elution but 2.33% (1/43) from the samples were detected for DEL phenotype that carried the RHD1227A allele by SSP-PCR. As a conclusion, the aim of this study was accomplished by the ability to detect DEL phenotype via molecular genotyping technique in distinguishing the DEL phenotype from truly D negative but not for freeze thaw elution technique.
format	Thesis
author	Nordin, Nordiana
author_facet	Nordin, Nordiana
author_sort	Nordin, Nordiana
title	Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin
title_short	Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin
title_full	Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin
title_fullStr	Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin
title_full_unstemmed	Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin
title_sort	detection of del phenotype in rhd negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / nordiana nordin
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/27365/ http://ir.uitm.edu.my/id/eprint/27365/1/TD_NORDIANA%20NORDIN%20HS%2015_5.pdf
first_indexed	2023-09-18T23:18:20Z
last_indexed	2023-09-18T23:18:20Z
_version_	1777419262690852864

Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin

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