Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman

INTRODUCTION: Pitfall in cytomorphological diagnostic can be defined as misdiagnosis or misinterpretation of result which may lead to false positive or negative thus affected treatment and caused possible morbidity· The occurrence error during the cytomorphological diagnosis is an artifact contamina...

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Main Author: Mohd Rahaman, Nur Hanina
Format: Thesis
Language:English
Published: 2015
Subjects:
Online Access:http://ir.uitm.edu.my/id/eprint/27371/
http://ir.uitm.edu.my/id/eprint/27371/1/TD_NUR%20HANINA%20MOHD%20RAHAMAN%20HS%2015_5.pdf
id uitm-27371
recordtype eprints
spelling uitm-273712020-01-21T03:17:29Z http://ir.uitm.edu.my/id/eprint/27371/ Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman Mohd Rahaman, Nur Hanina Cytology INTRODUCTION: Pitfall in cytomorphological diagnostic can be defined as misdiagnosis or misinterpretation of result which may lead to false positive or negative thus affected treatment and caused possible morbidity· The occurrence error during the cytomorphological diagnosis is an artifact contaminant. The possible artifact contaminants interference in the cytological slides might derived from patients, medical laboratory technologist and laboratory environment. The sources of artifact contaminant are lipsticks, pubic hairs, cotton fibers, KY Gel lubricant, compact powder, dust particles, gloves powder, pencil carbon and mascara. The general objective of this study is to determine the morphological characteristics of artefacts contaminant in cytological sample that can cause pitfalls in diagnostic. METHODS: The selected artefact contaminants were collected and put in the sterile container, containing normal human sputum as an adherence medium. Prepared slides were then stained using Papanicolaou (Pap) and May-Grunwald Giemsa (MGG), screened and the result were recorded by capturing the image using Leica DM750 Microscope equipped with an ICC 50 HD camera. Finally, the findings were compared to the normal, malignant and microorganisms cells via an establish cytology atlas and confirmed by qualified cytoscientists. RESULTS: The lipstick contaminant resemble tumor diathesis in present of malignancy background. Meanwhile, the pubic hairs resemble Wuchereria bancrofti parasite. The cotton cloth were resemble the Leptothrix bacteria. The KY Gel lubricant resemble to the mucus appearance. The compact powder has similar appearances to the comflaking artefacts. The dust particles resemble the keratotic cell. The gloves powder resemble with the cells in atrophic menopausal smear. The pencil carbon and mascara characteristics similar with the anthracotic pigment in black lung disease smears. CONCLUSION: The some morphological characteristics of artifact contaminants can mimic cytomorphological of human cells and these findings can be used as a reference in cytomorphological diagnostic to minimize a pitfall. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27371/1/TD_NUR%20HANINA%20MOHD%20RAHAMAN%20HS%2015_5.pdf Mohd Rahaman, Nur Hanina (2015) Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman. Degree thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Cytology
spellingShingle Cytology
Mohd Rahaman, Nur Hanina
Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman
description INTRODUCTION: Pitfall in cytomorphological diagnostic can be defined as misdiagnosis or misinterpretation of result which may lead to false positive or negative thus affected treatment and caused possible morbidity· The occurrence error during the cytomorphological diagnosis is an artifact contaminant. The possible artifact contaminants interference in the cytological slides might derived from patients, medical laboratory technologist and laboratory environment. The sources of artifact contaminant are lipsticks, pubic hairs, cotton fibers, KY Gel lubricant, compact powder, dust particles, gloves powder, pencil carbon and mascara. The general objective of this study is to determine the morphological characteristics of artefacts contaminant in cytological sample that can cause pitfalls in diagnostic. METHODS: The selected artefact contaminants were collected and put in the sterile container, containing normal human sputum as an adherence medium. Prepared slides were then stained using Papanicolaou (Pap) and May-Grunwald Giemsa (MGG), screened and the result were recorded by capturing the image using Leica DM750 Microscope equipped with an ICC 50 HD camera. Finally, the findings were compared to the normal, malignant and microorganisms cells via an establish cytology atlas and confirmed by qualified cytoscientists. RESULTS: The lipstick contaminant resemble tumor diathesis in present of malignancy background. Meanwhile, the pubic hairs resemble Wuchereria bancrofti parasite. The cotton cloth were resemble the Leptothrix bacteria. The KY Gel lubricant resemble to the mucus appearance. The compact powder has similar appearances to the comflaking artefacts. The dust particles resemble the keratotic cell. The gloves powder resemble with the cells in atrophic menopausal smear. The pencil carbon and mascara characteristics similar with the anthracotic pigment in black lung disease smears. CONCLUSION: The some morphological characteristics of artifact contaminants can mimic cytomorphological of human cells and these findings can be used as a reference in cytomorphological diagnostic to minimize a pitfall.
format Thesis
author Mohd Rahaman, Nur Hanina
author_facet Mohd Rahaman, Nur Hanina
author_sort Mohd Rahaman, Nur Hanina
title Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman
title_short Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman
title_full Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman
title_fullStr Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman
title_full_unstemmed Identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / Nur Hanina Mohd Rahaman
title_sort identification of artefact contamination morphological characteristics in minimizing pitfalls in cytology / nur hanina mohd rahaman
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/27371/
http://ir.uitm.edu.my/id/eprint/27371/1/TD_NUR%20HANINA%20MOHD%20RAHAMAN%20HS%2015_5.pdf
first_indexed 2023-09-18T23:18:21Z
last_indexed 2023-09-18T23:18:21Z
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