Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak

Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak

Background: Cytomorphological changes caused by delaying processing under room temperature (25°C) in sputum samples might contributed to pitfall diagnosis in respiratory tract cytology. Therefore, this study is conducted to determine the effects of delayed processing on cytomorphological changes in...

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Main Author: Ishak, Zulmaizura
Format: Thesis
Language:English
Published: 2015
Subjects:
Cytology
Online Access:http://ir.uitm.edu.my/id/eprint/27422/
http://ir.uitm.edu.my/id/eprint/27422/1/TD_ZULMAIZURA%20ISHAK%20HS%2015_5.pdf
id uitm-27422
recordtype eprints
spelling uitm-274222020-01-20T02:51:22Z http://ir.uitm.edu.my/id/eprint/27422/ Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak Ishak, Zulmaizura Cytology Background: Cytomorphological changes caused by delaying processing under room temperature (25°C) in sputum samples might contributed to pitfall diagnosis in respiratory tract cytology. Therefore, this study is conducted to determine the effects of delayed processing on cytomorphological changes in sputum samples. Methods: 6 sputum samples were selected from healthy students of Universiti Teknologi MARA Puncak Alam campus through the deep cough method. After samples collections, slides were prepared for Cell count, Papanicolaou stain, Maygrunwald giemsa stain (MGG) and Gram stain from samples in room temperature (25°C) for 0 hour (control),2 hours, 4 hours and 8 hours and were evaluated for the cytomorphological changes, cell count and bacterial invasion. The findings were observed for nuclear alterations, changes in cytoplasm, bacterial invasion and cell count. Images were captured by using Leica DM 750 microscope equipped with an ICC 50HD camera. Results and Discussion: From 2 hours onwards, morphological changes were observed on both nuclear and cytoplasmic. The results of total cell count for delayed processing time in room temperature in sputum samples showed a significant reduction (p<0.05) in number of cells, together with the increased in number of bacterial invasion. These provides evidence for the impact of processing time for delay process in room temperature on the quality of sputum samples for cytology. Conclusion: Cytomorphological changes were observed in sputum samples that were delayed in processing time at room temperature. This can impact the laboratory results and lead to misdiagnosis. Based on this finding, the study suggested that sputum samples for cytology should be immediately process after collection without delay. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27422/1/TD_ZULMAIZURA%20ISHAK%20HS%2015_5.pdf Ishak, Zulmaizura (2015) Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak. Degree thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Cytology
spellingShingle Cytology
Ishak, Zulmaizura
Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak
description Background: Cytomorphological changes caused by delaying processing under room temperature (25°C) in sputum samples might contributed to pitfall diagnosis in respiratory tract cytology. Therefore, this study is conducted to determine the effects of delayed processing on cytomorphological changes in sputum samples. Methods: 6 sputum samples were selected from healthy students of Universiti Teknologi MARA Puncak Alam campus through the deep cough method. After samples collections, slides were prepared for Cell count, Papanicolaou stain, Maygrunwald giemsa stain (MGG) and Gram stain from samples in room temperature (25°C) for 0 hour (control),2 hours, 4 hours and 8 hours and were evaluated for the cytomorphological changes, cell count and bacterial invasion. The findings were observed for nuclear alterations, changes in cytoplasm, bacterial invasion and cell count. Images were captured by using Leica DM 750 microscope equipped with an ICC 50HD camera. Results and Discussion: From 2 hours onwards, morphological changes were observed on both nuclear and cytoplasmic. The results of total cell count for delayed processing time in room temperature in sputum samples showed a significant reduction (p<0.05) in number of cells, together with the increased in number of bacterial invasion. These provides evidence for the impact of processing time for delay process in room temperature on the quality of sputum samples for cytology. Conclusion: Cytomorphological changes were observed in sputum samples that were delayed in processing time at room temperature. This can impact the laboratory results and lead to misdiagnosis. Based on this finding, the study suggested that sputum samples for cytology should be immediately process after collection without delay.
format Thesis
author Ishak, Zulmaizura
author_facet Ishak, Zulmaizura
author_sort Ishak, Zulmaizura
title Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak
title_short Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak
title_full Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak
title_fullStr Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak
title_full_unstemmed Effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / Zulmaizura Ishak
title_sort effects of delayed process on cytomorphological changes and cell count in sputum samples at room temperature / zulmaizura ishak
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/27422/
http://ir.uitm.edu.my/id/eprint/27422/1/TD_ZULMAIZURA%20ISHAK%20HS%2015_5.pdf
first_indexed 2023-09-18T23:18:26Z
last_indexed 2023-09-18T23:18:26Z
_version_ 1777419268715970560

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