Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi

Dengue, a mosquito-borne viral disease is a serious public health concern in many countries around the world. In Malaysia, suspected cases of dengue infection increase dramatically every year. Dengue infections cause a variable spectrum of manifestations ranging from dengue fever (DF) to dengue haem...

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Main Author:	Mohamad Zamberi, Zaiharina
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Public health. Hygiene. Preventive Medicine
Online Access:	http://ir.uitm.edu.my/id/eprint/27670/ http://ir.uitm.edu.my/id/eprint/27670/1/TM_ZAIHARINA%20MOHAMAD%20ZAMBERI%20MD%2016_5.pdf

id	uitm-27670
recordtype	eprints
spelling	uitm-276702020-01-24T01:00:42Z http://ir.uitm.edu.my/id/eprint/27670/ Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi Mohamad Zamberi, Zaiharina Public health. Hygiene. Preventive Medicine Dengue, a mosquito-borne viral disease is a serious public health concern in many countries around the world. In Malaysia, suspected cases of dengue infection increase dramatically every year. Dengue infections cause a variable spectrum of manifestations ranging from dengue fever (DF) to dengue haemorrhagic fever (DHF) with plasma leakage that may lead to hypovolaemic shock, called dengue shock syndrome (DSS). Recent studies suggested the potential role of Fc gamma receptors (FCGRs) in the pathogenesis of dengue. The aim of this study was to determine the frequencies of copy number variation (CNV) of the FCGR1A gene and correlate the FCGR1A profile with its gene expression and serum protein level in dengue patients with vascular leakage and those without vascular leakage. Extracted DNA, RNA and serum were isolated from blood and use to quantify FCGR1A CNV, gene expression and protein concentration using quantitative real-time polymerase chain reaction (qPCR), reverse transcription-qPCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) respectively. One hundred dengue patients with signs of vascular leakage (case) and without vascular leakage (control), and 50 healthy subjects (normal) tested. Statistical analysis revealed that CNV of FCGR1A was significantly associated with dengue when compared to the normal controls. The circulating FcyRlA were also found to be significantly different between dengue patients and normal controls. However, there was no difference between dengue patients and normal controls, and dengue patients with vascular leakage and those without vascular leakage. These findings suggested that the FcyRlA plays a role in the susceptibility of dengue infection, however, showed no evidence in contributing to the severity of dengue. Although the correlation between these three experiments were not significantly related, the role of FCGR1A in vascular leakage should not be excluded. Further studies with larger sample size is needed to confirm this finding. 2016 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27670/1/TM_ZAIHARINA%20MOHAMAD%20ZAMBERI%20MD%2016_5.pdf Mohamad Zamberi, Zaiharina (2016) Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Public health. Hygiene. Preventive Medicine
spellingShingle	Public health. Hygiene. Preventive Medicine Mohamad Zamberi, Zaiharina Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi
description	Dengue, a mosquito-borne viral disease is a serious public health concern in many countries around the world. In Malaysia, suspected cases of dengue infection increase dramatically every year. Dengue infections cause a variable spectrum of manifestations ranging from dengue fever (DF) to dengue haemorrhagic fever (DHF) with plasma leakage that may lead to hypovolaemic shock, called dengue shock syndrome (DSS). Recent studies suggested the potential role of Fc gamma receptors (FCGRs) in the pathogenesis of dengue. The aim of this study was to determine the frequencies of copy number variation (CNV) of the FCGR1A gene and correlate the FCGR1A profile with its gene expression and serum protein level in dengue patients with vascular leakage and those without vascular leakage. Extracted DNA, RNA and serum were isolated from blood and use to quantify FCGR1A CNV, gene expression and protein concentration using quantitative real-time polymerase chain reaction (qPCR), reverse transcription-qPCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) respectively. One hundred dengue patients with signs of vascular leakage (case) and without vascular leakage (control), and 50 healthy subjects (normal) tested. Statistical analysis revealed that CNV of FCGR1A was significantly associated with dengue when compared to the normal controls. The circulating FcyRlA were also found to be significantly different between dengue patients and normal controls. However, there was no difference between dengue patients and normal controls, and dengue patients with vascular leakage and those without vascular leakage. These findings suggested that the FcyRlA plays a role in the susceptibility of dengue infection, however, showed no evidence in contributing to the severity of dengue. Although the correlation between these three experiments were not significantly related, the role of FCGR1A in vascular leakage should not be excluded. Further studies with larger sample size is needed to confirm this finding.
format	Thesis
author	Mohamad Zamberi, Zaiharina
author_facet	Mohamad Zamberi, Zaiharina
author_sort	Mohamad Zamberi, Zaiharina
title	Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi
title_short	Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi
title_full	Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi
title_fullStr	Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi
title_full_unstemmed	Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi
title_sort	molecular and biochemical studies on fcgria susceptibility in dengue infection / zaiharina mohamad zamberi
publishDate	2016
url	http://ir.uitm.edu.my/id/eprint/27670/ http://ir.uitm.edu.my/id/eprint/27670/1/TM_ZAIHARINA%20MOHAMAD%20ZAMBERI%20MD%2016_5.pdf
first_indexed	2023-09-18T23:18:47Z
last_indexed	2023-09-18T23:18:47Z
_version_	1777419291159691264

Molecular and biochemical studies on FCGRIA susceptibility in dengue infection / Zaiharina Mohamad Zamberi

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