Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid

Studies examining the population genetics structure of Ae. albopictus based on mitochondrial DNA ND5 marker has not been elucidated at the local settings, despite its importance. Hence, this study was conducted to evaluate the intrapopulation and inter population genetic diversity among Ae. albopict...

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Main Author:	Abdul Khalid, Nor Adira
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Arthropoda Clinical pathology. Laboratory technique
Online Access:	http://ir.uitm.edu.my/id/eprint/28022/ http://ir.uitm.edu.my/id/eprint/28022/1/TD_NOR%20ADIRA%20ABDUL%20KHALID%20HS%2015_5.pdf

id	uitm-28022
recordtype	eprints
spelling	uitm-280222020-02-07T01:46:55Z http://ir.uitm.edu.my/id/eprint/28022/ Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid Abdul Khalid, Nor Adira Arthropoda Clinical pathology. Laboratory technique Studies examining the population genetics structure of Ae. albopictus based on mitochondrial DNA ND5 marker has not been elucidated at the local settings, despite its importance. Hence, this study was conducted to evaluate the intrapopulation and inter population genetic diversity among Ae. albopictus from Taman Bukit Kinrara, Selangor, a dengue cluster areas in Malaysia using a mitochondrial DNA marker. The genomic DNA of ten individual adult female Ae. albopictus mosquitoes and ten USM laboratory strains (USM LS)was extracted and analysed for DNA polymorphism based on the mitochondrial ND5 marker. 450 bp of amplified PCR products was obtained and sequenced. BLAST analysis showed high similarities with reference sequences from NCBI Gen Bank. Subsequently, the multiple nucleotide sequences were aligned using ClustaIX2.l. Haplotype network was constructed using TCS network in PopART software. Haplotypes generated was analyzed using DNaSP. Our findings revealed that Ae. albopictus population from Taman Bukit Kinrara, Selangor are genetically related at intra population and inter population levels with low genetic variation with Hd values of0.4368 and 0.5869 respectively. This study highlights the generation of 11 haplotypes from 49 sequences at 11 different polymorphic sites. The data obtained from this study may provides new fundamental knowledge regarding the genetic structure of local Ae. albopictus that could be utilised in forthcoming studies. 2016 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/28022/1/TD_NOR%20ADIRA%20ABDUL%20KHALID%20HS%2015_5.pdf Abdul Khalid, Nor Adira (2016) Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid. Degree thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Arthropoda Clinical pathology. Laboratory technique
spellingShingle	Arthropoda Clinical pathology. Laboratory technique Abdul Khalid, Nor Adira Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid
description	Studies examining the population genetics structure of Ae. albopictus based on mitochondrial DNA ND5 marker has not been elucidated at the local settings, despite its importance. Hence, this study was conducted to evaluate the intrapopulation and inter population genetic diversity among Ae. albopictus from Taman Bukit Kinrara, Selangor, a dengue cluster areas in Malaysia using a mitochondrial DNA marker. The genomic DNA of ten individual adult female Ae. albopictus mosquitoes and ten USM laboratory strains (USM LS)was extracted and analysed for DNA polymorphism based on the mitochondrial ND5 marker. 450 bp of amplified PCR products was obtained and sequenced. BLAST analysis showed high similarities with reference sequences from NCBI Gen Bank. Subsequently, the multiple nucleotide sequences were aligned using ClustaIX2.l. Haplotype network was constructed using TCS network in PopART software. Haplotypes generated was analyzed using DNaSP. Our findings revealed that Ae. albopictus population from Taman Bukit Kinrara, Selangor are genetically related at intra population and inter population levels with low genetic variation with Hd values of0.4368 and 0.5869 respectively. This study highlights the generation of 11 haplotypes from 49 sequences at 11 different polymorphic sites. The data obtained from this study may provides new fundamental knowledge regarding the genetic structure of local Ae. albopictus that could be utilised in forthcoming studies.
format	Thesis
author	Abdul Khalid, Nor Adira
author_facet	Abdul Khalid, Nor Adira
author_sort	Abdul Khalid, Nor Adira
title	Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid
title_short	Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid
title_full	Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid
title_fullStr	Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid
title_full_unstemmed	Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid
title_sort	haplotype analysis of field collected aedes albopictus from taman bukit kinrara, selangor using the nadh dehydrogenase subunit 5 (nd5) marker / nor adira abdul khalid
publishDate	2016
url	http://ir.uitm.edu.my/id/eprint/28022/ http://ir.uitm.edu.my/id/eprint/28022/1/TD_NOR%20ADIRA%20ABDUL%20KHALID%20HS%2015_5.pdf
first_indexed	2023-09-18T23:19:26Z
last_indexed	2023-09-18T23:19:26Z
_version_	1777419332117069824

Haplotype analysis of field collected aedes albopictus from Taman Bukit Kinrara, Selangor using the Nadh Dehydrogenase Subunit 5 (ND5) marker / Nor Adira Abdul Khalid

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