Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor

Polymorphonuclear neutrophils (PMNs) are white blood cells that play an important role as the first line of immune defence against pathogens. Neutrophils activation will trigger production of oxidative burst which is mediated by NADPH oxidase. The product of oxidative burst is a part of the powerful...

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Main Author:	Mohd Noor, Nurain Zulaika
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Clinical pathology. Laboratory technique
Online Access:	http://ir.uitm.edu.my/id/eprint/28090/ http://ir.uitm.edu.my/id/eprint/28090/1/TD_NURAIN%20ZULAIKHA%20MOHD%20NOOR%20HS%2015_5.pdf

id	uitm-28090
recordtype	eprints
spelling	uitm-280902020-02-03T04:11:11Z http://ir.uitm.edu.my/id/eprint/28090/ Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor Mohd Noor, Nurain Zulaika Clinical pathology. Laboratory technique Polymorphonuclear neutrophils (PMNs) are white blood cells that play an important role as the first line of immune defence against pathogens. Neutrophils activation will trigger production of oxidative burst which is mediated by NADPH oxidase. The product of oxidative burst is a part of the powerful bacteria killing system in PMNs. Neutrophils function is evaluated based on its ability to produce oxidative burst. The conventional nitroblue tetrazolium (NBT) assay is the gold standard method to evaluate neutrophils function. The NBT test is however laborious, complicated and skill-dependent. An alternative method called direct NBT assay was recently introduced. Thus, in the present study, the performance between conventional and direct NBT assays were compared. A total of 20 heparinized venous blood samples from normal individuals and one sample from chronic myeloid leukaemia (CML) patient were evaluated for neutrophil function by using both methods. There was a good correlation between conventional and direct NBT assays for percentage ofNBT positive cell with PMA stimulated cells (rs=0.97, P<O.OI) and unstimulated cells (rs=0.93, P<O.OI). Hence, the results from direct NBT assay is as comparable as that of conventional NBT assay. The direct NBT assay is quicker and simpler than the conventional NBT assay because whole blood is used thus it eliminates the preparation ofPMNs cell. In addition, sample volume of as small as 0.1 ml blood can be used which makes the direct NBT assay ideal for evaluation of neutrophil function in children and neonates. Therefore, the direct NBT assay may be a better choice or an alternative approach to evaluate neutrophil function as it is quicker, simpler, easier and less skill needed. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/28090/1/TD_NURAIN%20ZULAIKHA%20MOHD%20NOOR%20HS%2015_5.pdf Mohd Noor, Nurain Zulaika (2015) Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor. Degree thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Clinical pathology. Laboratory technique
spellingShingle	Clinical pathology. Laboratory technique Mohd Noor, Nurain Zulaika Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor
description	Polymorphonuclear neutrophils (PMNs) are white blood cells that play an important role as the first line of immune defence against pathogens. Neutrophils activation will trigger production of oxidative burst which is mediated by NADPH oxidase. The product of oxidative burst is a part of the powerful bacteria killing system in PMNs. Neutrophils function is evaluated based on its ability to produce oxidative burst. The conventional nitroblue tetrazolium (NBT) assay is the gold standard method to evaluate neutrophils function. The NBT test is however laborious, complicated and skill-dependent. An alternative method called direct NBT assay was recently introduced. Thus, in the present study, the performance between conventional and direct NBT assays were compared. A total of 20 heparinized venous blood samples from normal individuals and one sample from chronic myeloid leukaemia (CML) patient were evaluated for neutrophil function by using both methods. There was a good correlation between conventional and direct NBT assays for percentage ofNBT positive cell with PMA stimulated cells (rs=0.97, P<O.OI) and unstimulated cells (rs=0.93, P<O.OI). Hence, the results from direct NBT assay is as comparable as that of conventional NBT assay. The direct NBT assay is quicker and simpler than the conventional NBT assay because whole blood is used thus it eliminates the preparation ofPMNs cell. In addition, sample volume of as small as 0.1 ml blood can be used which makes the direct NBT assay ideal for evaluation of neutrophil function in children and neonates. Therefore, the direct NBT assay may be a better choice or an alternative approach to evaluate neutrophil function as it is quicker, simpler, easier and less skill needed.
format	Thesis
author	Mohd Noor, Nurain Zulaika
author_facet	Mohd Noor, Nurain Zulaika
author_sort	Mohd Noor, Nurain Zulaika
title	Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor
title_short	Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor
title_full	Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor
title_fullStr	Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor
title_full_unstemmed	Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor
title_sort	neutrophil function test: comparison between conventional nitroblue tetrazolium (nbt) and direct nbt assays / nurain zulaika mohd noor
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/28090/ http://ir.uitm.edu.my/id/eprint/28090/1/TD_NURAIN%20ZULAIKHA%20MOHD%20NOOR%20HS%2015_5.pdf
first_indexed	2023-09-18T23:19:35Z
last_indexed	2023-09-18T23:19:35Z
_version_	1777419341471416320

Neutrophil function test: comparison between Conventional Nitroblue Tetrazolium (NBT) and direct NBT assays / Nurain Zulaika Mohd Noor

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