Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar

Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar

Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve...

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Main Authors: Wan Hanafi, Wan Nurhayati, Abu Bakar, Nur Syahirah
Format: Article
Language:English
Published: Penerbit UiTM (UiTM Press) 2019
Subjects:
Animal biochemistry
DNA. Deoxyribonucleic acids
Online Access:http://ir.uitm.edu.my/id/eprint/28261/
http://ir.uitm.edu.my/id/eprint/28261/
http://ir.uitm.edu.my/id/eprint/28261/1/28261.pdf
id uitm-28261
recordtype eprints
spelling uitm-282612020-02-11T10:00:26Z http://ir.uitm.edu.my/id/eprint/28261/ Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar Wan Hanafi, Wan Nurhayati Abu Bakar, Nur Syahirah Animal biochemistry DNA. Deoxyribonucleic acids Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve PCR-amplifiable oil palm DNA. Oil palm leaves were cut and crushed before deposited onto the FTA card. An attempt was made by amplifying the EgSHP gene using a punch of FTA card as a DNA template. The successful outcome of PCR was measured by the presence of PCR amplicons on 1% agarose gel electrophoresis, indicate the genotype of oil palm fruit form. This present study demonstrates that the FTA card provides a versatile alternative to the study of oil palm genetics. Penerbit UiTM (UiTM Press) 2019 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/28261/1/28261.pdf Wan Hanafi, Wan Nurhayati and Abu Bakar, Nur Syahirah (2019) Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar. Scientific Research Journal, 16 (2). pp. 17-26. ISSN 1675-7009 https://doi.org/10.24191/srj.v16i2.6306
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Animal biochemistry
DNA. Deoxyribonucleic acids
spellingShingle Animal biochemistry
DNA. Deoxyribonucleic acids
Wan Hanafi, Wan Nurhayati
Abu Bakar, Nur Syahirah
Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar
description Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve PCR-amplifiable oil palm DNA. Oil palm leaves were cut and crushed before deposited onto the FTA card. An attempt was made by amplifying the EgSHP gene using a punch of FTA card as a DNA template. The successful outcome of PCR was measured by the presence of PCR amplicons on 1% agarose gel electrophoresis, indicate the genotype of oil palm fruit form. This present study demonstrates that the FTA card provides a versatile alternative to the study of oil palm genetics.
format Article
author Wan Hanafi, Wan Nurhayati
Abu Bakar, Nur Syahirah
author_facet Wan Hanafi, Wan Nurhayati
Abu Bakar, Nur Syahirah
author_sort Wan Hanafi, Wan Nurhayati
title Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar
title_short Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar
title_full Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar
title_fullStr Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar
title_full_unstemmed Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar
title_sort application of whatman fta card method in oil palm dna extraction and pcr analysis / wan nurhayati wan hanafi and nur syahirah abu bakar
publisher Penerbit UiTM (UiTM Press)
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/28261/
http://ir.uitm.edu.my/id/eprint/28261/
http://ir.uitm.edu.my/id/eprint/28261/1/28261.pdf
first_indexed 2023-09-18T23:19:54Z
last_indexed 2023-09-18T23:19:54Z
_version_ 1777419361503412224

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