The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah

This study was done to determine the most favourable pH for the viability of the Lactobacillus acidophilus and to determine the effect and most suitable volume of Kaffir Lime leaves extract for the growth of the bacteria. The Kaffir Lime Leaves was taken at Kuala Terengganu and the leaves were centr...

Full description

Bibliographic Details
Main Author:	Mohd Hanapiah, Nur Ain
Format:	Student Project
Language:	English
Published:	Faculty of Applied Science 2017
Subjects:	Extraction (Chemistry) QR Microbiology
Online Access:	http://ir.uitm.edu.my/id/eprint/28597/ http://ir.uitm.edu.my/id/eprint/28597/1/PPb_NUR%20AIN%20MOHD%20HANAPIAH%20AS%20N%2017_5.pdf

id	uitm-28597
recordtype	eprints
spelling	uitm-285972020-03-09T07:55:20Z http://ir.uitm.edu.my/id/eprint/28597/ The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah Mohd Hanapiah, Nur Ain Extraction (Chemistry) QR Microbiology This study was done to determine the most favourable pH for the viability of the Lactobacillus acidophilus and to determine the effect and most suitable volume of Kaffir Lime leaves extract for the growth of the bacteria. The Kaffir Lime Leaves was taken at Kuala Terengganu and the leaves were centrifuged to obtain the extract. Fresh milk, distilled water and Kaffir Lime leaves extract was added together for different samples with different volumes. The time taken for the fermentation was done for 12 hours and every 3 hours, the pH of the samples was taken. Plate count method was done and the result was recorded. For further conformation, the colony was observed under microscope by using gram staining to identify the morphology of the bacteria. Results show that the most suitable pH for the growth of Lactobacillus acidophilus is 4.42 to 4.43. Lactobacillus acidophilus growth the best at sample that contain 9 ml extract with O distilled water. For the samples that contain O ml to 6 ml extract of Kaffir Lime leaves, the growth of Lactobacillus acidophilus was growing linearly. However, the Lactobacillus acidophilus colony starts to decrease for samples contain 9 ml extract with addition of 3 ml, 6 ml, and 9 ml distilled water. This is due to over accumulation of lactic acid which not enhances the growth of bacteria. Then, Gram stain was done to identify the morphology of the Lactobacillus acidophilus. Under the microscope, purple colour, and vary size of Lactobacillus acidophilus can be observed. Faculty of Applied Science 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/28597/1/PPb_NUR%20AIN%20MOHD%20HANAPIAH%20AS%20N%2017_5.pdf Mohd Hanapiah, Nur Ain (2017) The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Extraction (Chemistry) QR Microbiology
spellingShingle	Extraction (Chemistry) QR Microbiology Mohd Hanapiah, Nur Ain The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah
description	This study was done to determine the most favourable pH for the viability of the Lactobacillus acidophilus and to determine the effect and most suitable volume of Kaffir Lime leaves extract for the growth of the bacteria. The Kaffir Lime Leaves was taken at Kuala Terengganu and the leaves were centrifuged to obtain the extract. Fresh milk, distilled water and Kaffir Lime leaves extract was added together for different samples with different volumes. The time taken for the fermentation was done for 12 hours and every 3 hours, the pH of the samples was taken. Plate count method was done and the result was recorded. For further conformation, the colony was observed under microscope by using gram staining to identify the morphology of the bacteria. Results show that the most suitable pH for the growth of Lactobacillus acidophilus is 4.42 to 4.43. Lactobacillus acidophilus growth the best at sample that contain 9 ml extract with O distilled water. For the samples that contain O ml to 6 ml extract of Kaffir Lime leaves, the growth of Lactobacillus acidophilus was growing linearly. However, the Lactobacillus acidophilus colony starts to decrease for samples contain 9 ml extract with addition of 3 ml, 6 ml, and 9 ml distilled water. This is due to over accumulation of lactic acid which not enhances the growth of bacteria. Then, Gram stain was done to identify the morphology of the Lactobacillus acidophilus. Under the microscope, purple colour, and vary size of Lactobacillus acidophilus can be observed.
format	Student Project
author	Mohd Hanapiah, Nur Ain
author_facet	Mohd Hanapiah, Nur Ain
author_sort	Mohd Hanapiah, Nur Ain
title	The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah
title_short	The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah
title_full	The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah
title_fullStr	The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah
title_full_unstemmed	The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah
title_sort	effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / nur ain mohd hanapiah
publisher	Faculty of Applied Science
publishDate	2017
url	http://ir.uitm.edu.my/id/eprint/28597/ http://ir.uitm.edu.my/id/eprint/28597/1/PPb_NUR%20AIN%20MOHD%20HANAPIAH%20AS%20N%2017_5.pdf
first_indexed	2023-09-18T23:20:36Z
last_indexed	2023-09-18T23:20:36Z
_version_	1777419405074890752

The effect of kaffir lime leaves extract on the ph and growth of lactobacillus acidophilus / Nur Ain Mohd Hanapiah

Similar Items