Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi
Oman depends on recycling its treated wastewater as a solution to overcome its limited water supplies and public health safety is therefore an important consideration. This study was carried out to assess the microbiological quality of treated wastewater samples from two wastewater treatment plan...
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| Format: | Thesis |
| Language: | English |
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2011
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| Online Access: | http://ir.uitm.edu.my/id/eprint/4265/ http://ir.uitm.edu.my/id/eprint/4265/1/TM_INTISAR%20SALIM%20AL-GHARIBI%20AS%2011_5%201.pdf |
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uitm-42652016-10-06T08:01:53Z http://ir.uitm.edu.my/id/eprint/4265/ Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi Salim Al-Gharibi, Intisar Water. Aquatic microbiology Qualities of water. Water quality Water purification. Water treatment and conditioning. Saline water conversion Oman depends on recycling its treated wastewater as a solution to overcome its limited water supplies and public health safety is therefore an important consideration. This study was carried out to assess the microbiological quality of treated wastewater samples from two wastewater treatment plants (WWTPs) in Oman, the Royal Hospital WWTP and Barka WWTP. This analysis was conducted on secondary and tertiary as well as de-chlorinated tertiary treated wastewater which were sampled during the summer and winter months from August 2007 to January 2008. The aims of the study were also to detect antibiotic and chlorine resistance in Escherichia coli using disk diffusion method. Sodium hypochlorite was used as a source of chlorine in the latter. Microbiological analysis showed that highest heterotrophic counts obtained from secondary, tertiary and de-chlorinated treated wastewater from both plants were from samples collected in August 2007, and the lowest were from samples collected in January 2008. This indicated that the heterotrophs were affected by the variation in seasonal temperatures. Analysis on the heterophic counts at three different incubation temperatures, 22oC, 37oC and 42oC indicated that the indigenous aquatic heterotrophs were more affected by the seasonal temperature variations compared to those of human and animal origin and thermotolerant heterotrophs. Total and faecal coliform counts in secondary, tertiary and dechlorinated tertiary treated wastewater samples from Barka WWTP were higher in the summer months and lower in the winter months but in the Royal Hospital WWTP this trend was not obvious in the tertiary de-chlorinated samples. The faecal coliform counts in the tertiary treated wastewater samples of both plants however, fell within the acceptable limits of Omani standards for use in watering public parks and landscapes. The enterocci counts in both plants were also higher in the summer months than in the winter months. Fifty seven confirmed E. coli isolates from both plants were tested for susceptibility to sixteen antibiotics namely Amikacin, Ampicillin, Chloramphenicol, Ciproflaxacin, Gentamyci, Cephotaxin, Kanamycin, Cephalothin, Minocylin, Neomycin, Nalidixicacid,S ulphamethoxazole, Streptomycin, Tetracycline, Tobramycin and Trimethoprim. All isolates from the Royal Hospital plant were resistant to at least two antibiotics, whereas those from the Barka plant were resistant to at least one. Tetracycline resistance was exhibited in 86.6% and 62.9% of the isolates from the Royal Hospital and Barka plants respectively. Multiple resistances to cephalothin and tetracycline were exhibited by 90% and 37% of the isolates from the Royal hospital and Barka plants respectively. All isolates were resistant to 0.5 mg/l sodium hypochlorite and the minimal inhibition concentration of 2.5 mg/l sodium hypochlorite was only exhibited in 24% of the isolates. 2011 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/4265/1/TM_INTISAR%20SALIM%20AL-GHARIBI%20AS%2011_5%201.pdf Salim Al-Gharibi, Intisar (2011) Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi. Masters thesis, Universiti Teknologi MARA. |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Teknologi MARA |
| building |
UiTM Institutional Repository |
| collection |
Online Access |
| language |
English |
| topic |
Water. Aquatic microbiology Qualities of water. Water quality Water purification. Water treatment and conditioning. Saline water conversion |
| spellingShingle |
Water. Aquatic microbiology Qualities of water. Water quality Water purification. Water treatment and conditioning. Saline water conversion Salim Al-Gharibi, Intisar Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi |
| description |
Oman depends on recycling its treated wastewater as a solution to overcome its
limited water supplies and public health safety is therefore an important
consideration. This study was carried out to assess the microbiological quality of
treated wastewater samples from two wastewater treatment plants (WWTPs) in
Oman, the Royal Hospital WWTP and Barka WWTP. This analysis was conducted
on secondary and tertiary as well as de-chlorinated tertiary treated wastewater which
were sampled during the summer and winter months from August 2007 to January
2008. The aims of the study were also to detect antibiotic and chlorine resistance in
Escherichia coli using disk diffusion method. Sodium hypochlorite was used as a
source of chlorine in the latter. Microbiological analysis showed that highest
heterotrophic counts obtained from secondary, tertiary and de-chlorinated treated
wastewater from both plants were from samples collected in August 2007, and the
lowest were from samples collected in January 2008.
This indicated that the heterotrophs were affected by the variation in seasonal
temperatures. Analysis on the heterophic counts at three different incubation
temperatures, 22oC, 37oC and 42oC indicated that the indigenous aquatic
heterotrophs were more affected by the seasonal temperature variations compared to
those of human and animal origin and thermotolerant heterotrophs. Total and faecal
coliform counts in secondary, tertiary and dechlorinated tertiary treated wastewater
samples from Barka WWTP were higher in the summer months and lower in the
winter months but in the Royal Hospital WWTP this trend was not obvious in the
tertiary de-chlorinated samples. The faecal coliform counts in the tertiary treated
wastewater samples of both plants however, fell within the acceptable limits of
Omani standards for use in watering public parks and landscapes. The enterocci
counts in both plants were also higher in the summer months than in the winter
months.
Fifty seven confirmed E. coli isolates from both plants were tested for susceptibility
to sixteen antibiotics namely Amikacin, Ampicillin, Chloramphenicol, Ciproflaxacin,
Gentamyci, Cephotaxin, Kanamycin, Cephalothin, Minocylin, Neomycin,
Nalidixicacid,S ulphamethoxazole, Streptomycin, Tetracycline, Tobramycin and
Trimethoprim. All isolates from the Royal Hospital plant were resistant to at least
two antibiotics, whereas those from the Barka plant were resistant to at least one.
Tetracycline resistance was exhibited in 86.6% and 62.9% of the isolates from the
Royal Hospital and Barka plants respectively. Multiple resistances to cephalothin and
tetracycline were exhibited by 90% and 37% of the isolates from the Royal hospital
and Barka plants respectively. All isolates were resistant to 0.5 mg/l sodium
hypochlorite and the minimal inhibition concentration of 2.5 mg/l sodium
hypochlorite was only exhibited in 24% of the isolates. |
| format |
Thesis |
| author |
Salim Al-Gharibi, Intisar |
| author_facet |
Salim Al-Gharibi, Intisar |
| author_sort |
Salim Al-Gharibi, Intisar |
| title |
Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi |
| title_short |
Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi |
| title_full |
Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi |
| title_fullStr |
Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi |
| title_full_unstemmed |
Microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant Escherichia coli from wastewater treatment plants in Oman / Intisar Salim Al-Gharibi |
| title_sort |
microbiological assessment of treated wastewater and detection of antibiotic and chlorine resistant escherichia coli from wastewater treatment plants in oman / intisar salim al-gharibi |
| publishDate |
2011 |
| url |
http://ir.uitm.edu.my/id/eprint/4265/ http://ir.uitm.edu.my/id/eprint/4265/1/TM_INTISAR%20SALIM%20AL-GHARIBI%20AS%2011_5%201.pdf |
| first_indexed |
2023-09-18T22:46:54Z |
| last_indexed |
2023-09-18T22:46:54Z |
| _version_ |
1777417285609193472 |