Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman

The treatment and disposal of domestic sludge is an expensive and environmentally sensitive problem. It is also a growing problem since sludge production will continue to increase as new wastewater treatment plants are built due to population increase. The large volume of domestic sludge produced ha...

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Main Author:	Othman, Norzila
Format:	Thesis
Language:	English
Published:	2010
Subjects:	Environmental effects of industries and plants Special types of environment. Including soil pollution, air pollution, noise pollution
Online Access:	http://ir.uitm.edu.my/id/eprint/5468/ http://ir.uitm.edu.my/id/eprint/5468/1/TP_NORZILA%20OTHMAN%20EC%2010_5%201.pdf

id	uitm-5468
recordtype	eprints
spelling	uitm-54682016-11-26T06:30:12Z http://ir.uitm.edu.my/id/eprint/5468/ Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman Othman, Norzila Environmental effects of industries and plants Special types of environment. Including soil pollution, air pollution, noise pollution The treatment and disposal of domestic sludge is an expensive and environmentally sensitive problem. It is also a growing problem since sludge production will continue to increase as new wastewater treatment plants are built due to population increase. The large volume of domestic sludge produced had made it difficult for many countries including Malaysia to assure complete treatment of the sludge before discharging to the receiving environment. Domestic sludge contains diverse range of pollutants such as pathogen, inorganic and organic compounds. These pollutants are toxic, mutagenic or carcinogenic and may threaten human health. Improper disposal and handling of sludge may pose serious impact to the environment especially on soil and water cycles. Previous studies on Malaysian domestic sludge only reported on bulk parameters and heavy metals. Thus, no study reported on organic micro pollutants, namely, polycylic aromatic hydrocarbons (PAHs). Their recalcitrance and persistence make them problematic environmental contaminants. Microbial degradation is considered to be the primary mechanism of PAHs removal from the environment. Much has been reported on biodegradation of PAHs in several countries but there is a lack of information quantitative on this subject in Malaysia. This study is carried out to understand the nature of domestic sludge and to provide a better understanding on the biodegradation processes of PAHs. The methodology of this study comprised field activities, laboratory work and mathematical modelling. Field activities involved sampling of domestic sludge from Kolej Mawar, Universiti Teknologi MARA, Shah Alam, Selangor. Laboratory activities include seven phases of experimental works. First phase is characterization study of domestic sludge based on bulk parameters, heavy metals and PAHs. Second phase is enrichment and purification of bacteria isolated from domestic sludge using single PAHs and mixed PAHs as growth substrate. This was followed by identification of bacteria using BIOLOG system. The fourth phase focussed on turbidity test to monitor growth rate of the isolated bacteria. Preliminary degradation study involves optimization of the process at different substrate concentration, bacteria concentration, pH and temperature. The optimum conditions established from optimization study were used in degradation study. In biodegradation study, two experimental conditions were performed. These conditions include using bacteria isolated from single PAHs as substrate and bacteria isolated from mixed PAHs. Protein and pH tests were done during degradation study. 2010 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/5468/1/TP_NORZILA%20OTHMAN%20EC%2010_5%201.pdf Othman, Norzila (2010) Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman. PhD thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Environmental effects of industries and plants Special types of environment. Including soil pollution, air pollution, noise pollution
spellingShingle	Environmental effects of industries and plants Special types of environment. Including soil pollution, air pollution, noise pollution Othman, Norzila Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
description	The treatment and disposal of domestic sludge is an expensive and environmentally sensitive problem. It is also a growing problem since sludge production will continue to increase as new wastewater treatment plants are built due to population increase. The large volume of domestic sludge produced had made it difficult for many countries including Malaysia to assure complete treatment of the sludge before discharging to the receiving environment. Domestic sludge contains diverse range of pollutants such as pathogen, inorganic and organic compounds. These pollutants are toxic, mutagenic or carcinogenic and may threaten human health. Improper disposal and handling of sludge may pose serious impact to the environment especially on soil and water cycles. Previous studies on Malaysian domestic sludge only reported on bulk parameters and heavy metals. Thus, no study reported on organic micro pollutants, namely, polycylic aromatic hydrocarbons (PAHs). Their recalcitrance and persistence make them problematic environmental contaminants. Microbial degradation is considered to be the primary mechanism of PAHs removal from the environment. Much has been reported on biodegradation of PAHs in several countries but there is a lack of information quantitative on this subject in Malaysia. This study is carried out to understand the nature of domestic sludge and to provide a better understanding on the biodegradation processes of PAHs. The methodology of this study comprised field activities, laboratory work and mathematical modelling. Field activities involved sampling of domestic sludge from Kolej Mawar, Universiti Teknologi MARA, Shah Alam, Selangor. Laboratory activities include seven phases of experimental works. First phase is characterization study of domestic sludge based on bulk parameters, heavy metals and PAHs. Second phase is enrichment and purification of bacteria isolated from domestic sludge using single PAHs and mixed PAHs as growth substrate. This was followed by identification of bacteria using BIOLOG system. The fourth phase focussed on turbidity test to monitor growth rate of the isolated bacteria. Preliminary degradation study involves optimization of the process at different substrate concentration, bacteria concentration, pH and temperature. The optimum conditions established from optimization study were used in degradation study. In biodegradation study, two experimental conditions were performed. These conditions include using bacteria isolated from single PAHs as substrate and bacteria isolated from mixed PAHs. Protein and pH tests were done during degradation study.
format	Thesis
author	Othman, Norzila
author_facet	Othman, Norzila
author_sort	Othman, Norzila
title	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_short	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_full	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_fullStr	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_full_unstemmed	Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman
title_sort	polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / norzila othman
publishDate	2010
url	http://ir.uitm.edu.my/id/eprint/5468/ http://ir.uitm.edu.my/id/eprint/5468/1/TP_NORZILA%20OTHMAN%20EC%2010_5%201.pdf
first_indexed	2023-09-18T22:47:07Z
last_indexed	2023-09-18T22:47:07Z
_version_	1777417298555961344

Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition / Norzila Othman

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