Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak

This study is attempts to determine antimalarial activity of Q. borneensis extracts against the intraerythrocytic phase of P. berghei NK65. Parts of Q. borneensis was extracted by cold maceration and soxhlet in 10% aqueous methanol before partitioned with n-Hexane, and chloroform. The extratcs were...

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Main Authors:	Ghazali, Ahmad Rohi, Nuddin, Julenah Ag, Sabireen, Siti Nurfarhana, Wan Razali, Wan Marahaini
Format:	Article
Language:	English
Published:	Faculty of Business Management 2013
Subjects:	Organic chemistry Malaria
Online Access:	http://ir.uitm.edu.my/id/eprint/8749/ http://ir.uitm.edu.my/id/eprint/8749/1/AJ_MOHAMAD%20AZMI%20NIAS%20AHMAD%20IJUS%2013.pdf

id	uitm-8749
recordtype	eprints
spelling	uitm-87492017-08-03T04:03:05Z http://ir.uitm.edu.my/id/eprint/8749/ Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak Ghazali, Ahmad Rohi Nuddin, Julenah Ag Sabireen, Siti Nurfarhana Wan Razali, Wan Marahaini Organic chemistry Malaria This study is attempts to determine antimalarial activity of Q. borneensis extracts against the intraerythrocytic phase of P. berghei NK65. Parts of Q. borneensis was extracted by cold maceration and soxhlet in 10% aqueous methanol before partitioned with n-Hexane, and chloroform. The extratcs were subjected to colorimetric Plasmodium Lactate Dehydrogenase Assay (pLDH). The extract with most potential IC50 was later evaluated in synchronization test. It was found that hexane soxhlet extract of root, (Qb4), and chloroform soxhlet extract of bark, (Qb7) showed potential, at the values of IC50 0.039μg/ml and 0.001μg/ml, respectively. In the synchronization test, Qb7 extract was found to be active against all three stages of parasites in the intraerythrocytic phase, with the IC50 value for the early, late trophozoite and schizont at 1.964 μg/ml, 0.0009μg/ml and 0.007 μg/ml respectively. This study coulclude that Q. borneensis might possess biologically active quassinoids which could contribute to their antimalarial activities. Faculty of Business Management 2013 Article NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/8749/1/AJ_MOHAMAD%20AZMI%20NIAS%20AHMAD%20IJUS%2013.pdf Ghazali, Ahmad Rohi and Nuddin, Julenah Ag and Sabireen, Siti Nurfarhana and Wan Razali, Wan Marahaini (2013) Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak. International Journal of Undergraduates Studies, 2 (2). pp. 24-31. ISSN 2289-4242
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Organic chemistry Malaria
spellingShingle	Organic chemistry Malaria Ghazali, Ahmad Rohi Nuddin, Julenah Ag Sabireen, Siti Nurfarhana Wan Razali, Wan Marahaini Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak
description	This study is attempts to determine antimalarial activity of Q. borneensis extracts against the intraerythrocytic phase of P. berghei NK65. Parts of Q. borneensis was extracted by cold maceration and soxhlet in 10% aqueous methanol before partitioned with n-Hexane, and chloroform. The extratcs were subjected to colorimetric Plasmodium Lactate Dehydrogenase Assay (pLDH). The extract with most potential IC50 was later evaluated in synchronization test. It was found that hexane soxhlet extract of root, (Qb4), and chloroform soxhlet extract of bark, (Qb7) showed potential, at the values of IC50 0.039μg/ml and 0.001μg/ml, respectively. In the synchronization test, Qb7 extract was found to be active against all three stages of parasites in the intraerythrocytic phase, with the IC50 value for the early, late trophozoite and schizont at 1.964 μg/ml, 0.0009μg/ml and 0.007 μg/ml respectively. This study coulclude that Q. borneensis might possess biologically active quassinoids which could contribute to their antimalarial activities.
format	Article
author	Ghazali, Ahmad Rohi Nuddin, Julenah Ag Sabireen, Siti Nurfarhana Wan Razali, Wan Marahaini
author_facet	Ghazali, Ahmad Rohi Nuddin, Julenah Ag Sabireen, Siti Nurfarhana Wan Razali, Wan Marahaini
author_sort	Ghazali, Ahmad Rohi
title	Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak
title_short	Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak
title_full	Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak
title_fullStr	Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak
title_full_unstemmed	Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak
title_sort	ex vivo antiplasmodial activity of quassia borneensis noot. (simaroubaceae) extracts against mice erythrocytes infected by plasmodium berghei nk 65 / ahmad rohi ghazali, julenah ag nuddin, siti nurfarhana sabireen, wan marahaini wan razali and shafariatul akmar ishak
publisher	Faculty of Business Management
publishDate	2013
url	http://ir.uitm.edu.my/id/eprint/8749/ http://ir.uitm.edu.my/id/eprint/8749/1/AJ_MOHAMAD%20AZMI%20NIAS%20AHMAD%20IJUS%2013.pdf
first_indexed	2023-09-18T22:47:27Z
last_indexed	2023-09-18T22:47:27Z
_version_	1777417319841005568

Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak

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