Analysis of porcine DNA in several food products
Species identification in food products has grown interest in recent years since these foodstuffs are liable targets for fraudulent labelling. Consumers of Halal products typically buying those with a Halal logo stamped on the packaging without doubt for its authenticity. DNA-based methods on PCR...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2015
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| Online Access: | http://journalarticle.ukm.my/10336/ http://journalarticle.ukm.my/10336/ http://journalarticle.ukm.my/10336/1/44_1_02.pdf |
| Summary: | Species identification in food products has grown interest in recent years since these foodstuffs are liable targets for fraudulent
labelling. Consumers of Halal products typically buying those with a Halal logo stamped on the packaging without doubt for
its authenticity. DNA-based methods on PCR amplification were used to identify the presence of porcine DNA in the processed
food samples. Genomic DNA of porcine was extracted using DNA-Sorb-C and subjected to PCR amplification targeting the
mitochondrial 12S rDNA gene and porcine leptin gene (LEP). Out of 66 samples, 37 samples showed amplified product of
387 bp with mitochondrial 12S rDNA gene and 59 samples showed amplified products of 152 bp with LEP. The verification
for Halal authentication of food products is significant for economical, religious and public health concerns. |
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